BV2 microglial cells에서 ERK를 통한 고삼의 Tnf alpha 생성 억제효과

ERK mediated suppressive effects of Sophora flavescens on Tnf alpha production in BV2 microglial cells

  • 김수철 (경희대학교 고황 의학 연구소) ;
  • 한미영 (경희대학교 고황 의학 연구소) ;
  • 박혜정 (경희대학교 의과대학) ;
  • 정경희 (경희대학교 고황 의학 연구소)
  • Kim, Soo-Cheol (Kohwang Medical Research Institute, College of Medicine, Kyung Hee University) ;
  • Han, Mi-Young (Kohwang Medical Research Institute, College of Medicine, Kyung Hee University) ;
  • Park, Hae-Jeong (Department of pharmacology, College of Medicine, KyungHee University) ;
  • Jung, Kyung-Hee (Kohwang Medical Research Institute, College of Medicine, Kyung Hee University)
  • 발행 : 2007.06.30

초록

Objectives : Sophora flavescens (SF) is widely used in traditional herbal medicine in Korea and is well recognized for its anti-inflammatory effect. However, its effect on Tumornecrosis factor alpha (Tnf) production in BV2 microglial cell is not yet known. Methods : We investigated the effect of SF on the production and expression of Tnf, a well known inflammatory mediator, in lipopolysaccaride (LPS)-activated BV2 microglial cells. Results : The LPS-induced Tnf production was markedly reduced by treatment with SF (50 ${\mu}g/ml$). In reverse transcription polymerase chain reaction (RT-PCR) analysis, SF suppressed the LPS activated expression of Tnf mRNA. In addition, Western blot analysis confirmed that SF suppressed the expression of Tnf. Sophora flavescens also inhibited the LPS-induced phosphylation of extracellular signal-regulated kinases (ERK), which mediate the Tnfproduction signaling pathway whereas LPS-induced phosphylation of p38 mitogen activated protein kinase (p38 MAPK), and c-Jun NH2-terminal kinases (JNK) was not inhibited by SF, which implies that SF suppresses LPS-induced Tnf production via the ERK mediated pathway. Conclusion : Taken together, these findings indicated that SF inhibits LPS-induce Tnf production, and that this inhibitory effect is mediated via the ERK pathway.

키워드