The Korea Journal of Herbology (대한본초학회지)
- Volume 22 Issue 1
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- Pages.7-12
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- 2007
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- 1229-1765(pISSN)
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- 2288-7199(eISSN)
Effects of Hwangryunhaedok-tang on DNA Damage, Antioxidant Enzymes Expression and Acetylcholinesterase Activity
황연해독탕(黃連解毒湯)의 산화적 DNA 손상에 대한 보호효과 및 항산화효소계의 발현과 Acetylcholinesterase 활성에 미치는 영향
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Moon, Jin-Young
(College of Oriental Medicine, Dongguk University)
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문진영
(동국대학교 한의과대학)
- Published : 2007.03.30
Abstract
Objectives : In Alzheimer's disease(AD), free radical oxidative stress caused by amyloid beta-peptide may lead to DNA damage, neuronal dysfunction, neurotoxicity and cell death, Hwangryunhaedok-tang(HHT) is traditionally used for the treatment of pyrogenetic diseases. To develop a new anti-AD drug from natural herb, HHT was selected and extracted in this study. Methods : The antioxidant activities of HHT water extract powder were examined by hydroxyl radical-induced DNA strand nicking assay, and antioxidative enzymes expression assay in H4IIE cell. In addition, HHT was examined for the inhibitory effect on the acetylcholinesterase(AChE) using by Ellman's coupled assay. Results: The HHT exhibit DNA protective effect in the hydroxyl radical-induced DNA Strand nicking assay, mRNA expression of superoxide dismutase and glutathione peroxidase were recovered at a normal level by HHT treatment in H4IIE cell. Furthermore, water extract of HHT showed inhibitory effect on AChE activity. Conclusion : These results suggest that HHT may be effective in delaying and preventing AD progression related to the free radical-induced DNA damage and AChE activity.