Journal of Oriental Neuropsychiatry (동의신경정신과학회지)

The Korean Society of Oriental Neuropsychiatry (대한한방신경정신과학회)
권호 표지

Inhibitory effect of Alzheimer's in APP-induced Neuro 2A cells by JangWonHwanGagambang(JWHG) water extract

장원환가감방 수추출물(水抽出物)이 아밀로이드 전구단백질으로 유도된 생쥐의 신경아세포주에서의 항치매 효과

  • Kim, Sang-Tae (Division of Nanoscience, Ewha Womans of University) ;
  • Lee, Jong-Hwa (Dept. of Pediatrics, College of medicine, Wonkwang university) ;
  • Kim, Tae-Hyeon (Dept. of Neuropsychiaty, Colege of Oriental Medicine, Wonkwang University) ;
  • Son, Hyeong-Jin (Division of BioScience-Pharmacy, Ewha Womans of University) ;
  • Han, Pyeong-Leem (Division of Nanoscience, Ewha Womans of University) ;
  • Kang, Hyung-Won (Dept. of Neuropsychiaty, Colege of Oriental Medicine, Wonkwang University)
  • 김상태 (이화여자대학교 자연대학 나노과학부 뇌신경 분자연구실) ;
  • 이종화 (원광대학교 의과대학 소아과학교실) ;
  • 김태헌 (원광대학교 한의과대학 한방신경정신과교실) ;
  • 손형진 (이화여자대학교 생명 약학부 뇌신경연구실) ;
  • 한평림 (이화여자대학교 자연대학 나노과학부 뇌신경 분자연구실) ;
  • 강형원 (원광대학교 한의과대학 한방신경정신과교실)
  • Published : 2007.07.30
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Abstract

Objective : Recent studies indicate that the deposition of ${\beta}-amyloid$ ($A{\beta}$) is related in the pathogenesis of Alzheimer's disease (AD), but the underlying mechanism is still not clear. Method : To investigate the potential cellular functions of APP and water extract of the JangwonHwangagambang (JWHG), we use as in vitro model, neuro 2A cells were treated with either JWHG or its oriental medicines, and the effect in APP expression was determined by MTT and LDH assay. JWHG have been shown to be neuroprotective in different model systems. We asked whether JWHG treatment would influence cell survival and AD-like pathology in APP-induced neuronal cells. Result : JWHG and water extracts of some oriental medicine has attenuated high cell death in vitro. JWHG-treated cells increased percentage of cell survival more longly than controls. JWHG had significantly increas neurite outgrowth in the as compared to control cells. Conclusion : These results suggest that JWHG prevent APP-induced neurotoxicity through attenuating oxidative stress, and may be useful as potential therapeutic agents for AD.

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