생명과학회지 (Journal of Life Science)

한국생명과학회 (Korean Society of Life Science)
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(α-Amylase가 고정화된 Magnetic Nanofiber를 이용한 전분 분해공정 개발

Development of (α-Amylase Coated Magnetic Nanofiber for the Hydrolysis of Starch.

  • 김현 (조선대학교 생명화학공학과) ;
  • 이중헌 (조선대학교 생명화학공학과)
  • Kim, Hyun (Department of Chemical and Biochemical Engineering Chosun University) ;
  • Lee, Jung-Heon (Department of Chemical and Biochemical Engineering Chosun University)
  • 발행 : 2007.09.30
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초록

자석으로 분리가 가능한 ${\alpha}-Amylase$가 코팅된 나노고분자를 제조하여 녹말의 분해공정에 활용하였다. 본 연구에서 개발된 고정화 효소의 안정성은 크게 증가하여 상온에서 200rpm으로 교반하면서 보관한 경우 30일 동안에 92.7%의 활성도를 유지하였다 . 고정화 효소를 자석을 이용하여 재사용한 경우 10 회 동안 사용했을 경우 95.2%의 활성도 회수율을 보 여 효소의 재사용 가능성을 확인시켜 주었다 . 고정화 효소 0.5mg을 사용하여 녹말 분해 공정 에 활용하였을 때 2 ml의 40 g/l 녹말 용액을 40분만에 완전히 분해 시켰다, 이러한 고정화 효소를 사용하여 연속 효소반응기를 개발하여 녹말 분해공정에 활용한 결과 체류시간을 1시간으로 하였을 때 녹말 30 g/l를 76% 분해시켜 산업적으로 활용 가능성을 보여 주었다.

Magnetically separable enzyme-coated nanofibers were developed for the hydrolysis of starch. Stability of ${\alpha}-amylase-coated$ nanofiber was greatly improved and its residual activity was maintained over 92.7% after 32 days incubation at room temperature and under shaking conditions (200 rpm). The recovery of enzyme was high and enzyme activity after 10 recycle was 95.2% of its original activity. Developed enzyme-coated nanofibers were used for the hydrolysis of starch. When 0.5 mg of magnetically separable enzyme nanofibers was used, 40 g/l of starch (2 ml) was completely degraded within 40 min. The continuous enzyme reactor was developed and used for starch hydrolysis and 76% of starch (30 g/l) was hydrolyzed with 1 hr residence time.

키워드

참고문헌

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피인용 문헌

  1. Nanomaterials as novel supports for the immobilization of amylolytic enzymes and their applications: A review vol.3, pp.1, 2017, https://doi.org/10.1515/boca-2017-0004