Preventive Nutrition and Food Science

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Cyanidin and Cyanidin-3-O-β-D-glucoside Suppress the Inflammatory Responses of Obese Adipose Tissue by Inhibiting the Release of Chemokines MCP-1 and MRP-2

  • Choe, Mi-Ran (Department of Food Science and Nutrition, University of Ulsan) ;
  • Kang, Ji-Hye (Department of Food Science and Nutrition, University of Ulsan) ;
  • Yoo, Hoon (Department of Pharmacology and Dental Therapeutics, College of Dentistry, Chosun University) ;
  • Yang, Chae-Ha (Department of Physiology, College of Oriental Medicine, Daegu Haany University) ;
  • Kim, Mi-Ok (Department of Health Diet, Daegu Health College) ;
  • Yu, Ri-Na (Department of Food Science and Nutrition, University of Ulsan) ;
  • Choe, Suck-Young (Department of Food Science and Nutrition, University of Ulsan)
  • Published : 2007.09.30
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Abstract

Obesity-induced inflammation plays a crucial role in obesity-related pathologies such as type II diabetes and atherosclerosis. Adipose tissue macrophages and the cell-derived proinflammatory chemokines are key components in augmenting inflammatory responses in obesity. Anthocyanins such as cyanidin and $cyanidin-3-O-{\beta}-D-glucoside$ (C3G) are known to elicit anti-inflammatory activities by suppressing the production of proinflammatory mediators such as tumor necrosis factor alpha and nitric oxide in LPS-stimulated macrophages. In the present study, we investigated whether cyanidin and C3G have the potential to suppress the inflammatory responses of adipose cells. Cyanidin and C3G not only suppressed the migration of RAW 264.7 macrophages induced by mesenteric adipose tissue-conditioned medium, but also inhibited the activation of the cells to produce inflammatory chemokines such as monocyte chemoattractant protein-1 (MCP-1) and macrophage inflammatory protein-related protein-2 (MRP-2) in a dose-dependent manner. Cyanidin and C3G also inhibited the release of MCP-1 and MRP-2 from adipocytes and/or macrophages. These findings suggest that cyanidin and C3G may suppress the inflammatory responses of adipose tissue in obesity.

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References

  1. Weisberg SP, McCann D, Desai M, Rosenbaum M, Leibel RL, Ferrante Jr AW. 2003. Obesity is associated with macrophage accumulation in adipose tissue. J Clin Invest 112: 1796-1808 https://doi.org/10.1172/JCI19246.
  2. Xu H, Barnes GT, Yang Q, Tan G, Yang D, Chou CJ, Sole J, Nichols A, Ross JS, Tartaglia LA, Chen H. 2003. Chronic inflammation in fat plays a crucial role in the development of obesity-related insulin resistance. J Clin Invest 112: 1821-1830 https://doi.org/10.1172/JCI19451.
  3. Hotamisligil GS, Shargill NS, Spiegelman BM. 1993. Adipose expression of tumor necrosis factor-alpha: direct role in obesity-linked insulin resistance. Science 259: 87-91 https://doi.org/10.1126/science.7678183
  4. Fontana L, Eagon JC, Trujillo ME, Scherer PE, Klein S. 2007. Visceral fat adipokine secretion is associated with systemic inflammation in obese humans. Diabetes 56: 1010-1013 https://doi.org/10.2337/db06-1656
  5. Sartipy P, Loskutoff DJ. 2003. Monocyte chemoattractant protein 1 in obesity and insulin resistance. Proc Natl Acad Sci USA 100: 7265-7270
  6. Yu R, Park JS, Kawada T, Kwon BS. Alteration of a macrophages inflammatory protein-related protein-2 (MRP-2) response by high fat and cholesterol diet in mice. 2002. Life Sci 70: 2535-2545 https://doi.org/10.1016/S0024-3205(02)01526-6
  7. Yu R, Kim CS, Kwon BS, Kawada T. 2006. Mesenteric adipose tissue-derived monocyte chemoattractant protein-1 plays a crucial role in adipose tissue macrophage migration and activation in obese mice. Obesity (Silver Spring) 14: 1353-1362 https://doi.org/10.1038/oby.2006.153
  8. Hou DX, Yanagita T, Uto T, Masuzaki S, Fujii M. 2005. Anthocyanidins inhibit cyclooxygenase-2 expression in LPS-evoked macrophages: structure-activity relationship and molecular mechanisms involved. Biochem Pharmacol 70: 417-425 https://doi.org/10.1016/j.bcp.2005.05.003
  9. Pergola C, Rossi A, Dugo P, Cuzzocrea S, Sautebin L. 2006. Inhibition of nitric oxide biosynthesis by anthocyanin fraction of blackberry extract. Nitric Oxide 15: 30-39 https://doi.org/10.1016/j.niox.2005.10.003
  10. Tsuda T, Horio F, Uchida K, Aoki H, Osawa T. 2003. Cyanidin 3-O-beta-D-glucoside-rich purple corn color prevents obesity and ameliorates hyperglycemia in mice. J Nutr 133: 2125-2130 https://doi.org/10.1093/jn/133.7.2125
  11. Jayaprakasam B, Olson LK, Schutzki RE, Tai MH, Nair MG. 2006. Amelioration of obesity and glucose intolerance in high-fat-fed C57BL/6 mice by anthocyanins and ursolic acid in Cornelian cherry (Cornus mas). J Agric Food Chem 54: 243-248 https://doi.org/10.1021/jf0520342
  12. Tsuda T, Ueno Y, Aoki H, Koda T, Horio F, Takahashi N, Kawada T, Osawa T. 2004. Anthocyanin enhances adipocytokine secretion and adipocyte-specific gene expression in isolated rat adipocytes. Biochem Biophys Res Commun 316: 149-157 https://doi.org/10.1016/j.bbrc.2004.02.031
  13. Kim CS, Kawada T, Yoo H, Kwon BS, Yu R. 2003. Macrophage inflammatory protein-related protein-2, a novel CC chemokine, can regulate preadipocyte migration and adipocyte differentiation. FEBS Lett 548: 125-130 https://doi.org/10.1016/S0014-5793(03)00728-2
  14. Bruun JM, Lihn AS, Madan AK, Pedersen SB, Schiott KM, Fain JN, Richelsen B. 2004. Higher production of IL-8 in visceral vs. subcutaneous adipose tissue. Mplication of nonadipose cells in adipose tissue. Am J Physiol Endocrinol Metab 286: E8-13 https://doi.org/10.1152/ajpendo.00269.2003
  15. Tsuda T, Ueno Y, Yoshikawa T, Kojo H, Osawa T. 2006. Microarray profiling of gene expression in human adipocytes in response to anthocyanins. Biochem Pharmacol 71: 1184-1197 https://doi.org/10.1016/j.bcp.2005.12.042

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