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Improvement on the Quality and Functionality of Red Tanner Crab Cooking Drip Using Commercial Enzymes

효소분해의 의한 붉은 대게 자숙액의 품질 및 기능성 개선

  • Kang, Kyung-Tae (Dept. of Seafood Bioscience and Technology/Institute of Marine Industry, Gyeongsang National University) ;
  • Heu, Min-Soo (Dept. of Food Science/Institute of Marine Industry, Gyeongsang National University) ;
  • Kim, Jin-Soo (Dept. of Seafood Bioscience and Technology/Institute of Marine Industry, Gyeongsang National University)
  • 강경태 (경상대학교 해양식품생명공학과/해양산업연구소) ;
  • 허민수 (경상대학교 식품과학과/해양산업연구소) ;
  • 김진수 (경상대학교 해양식품생명공학과/해양산업연구소)
  • Published : 2007.08.30

Abstract

For the improvement on the quality and functionality of red tanner crab cooking drip, the preparation of hydrolysates from red crab cooking drip using commercial enzymes (Alcalase, Flavourzyme, Neutrase and Protamex) was attempted and its taste, nutritional and functional characteristics were also investigated. According to the results of heavy metal contents and proximate composition, red tanner crab cooking drip (RTCCD) could be used as a food resource. From the results of the trichloroacetic acid soluble index (TSI), angiotensin I converting enzyme (ACE) inhibiting activity and antioxidative activity, RTCCD hydrolysates incubated with Alcalase for 2 hrs was superior to the other one-step hydrolysates. There were no differences in the ACE inhibiting activity and antioxidative activity between one-step hydrolysates, which was incubated with Alcalase for 2 hrs, and two-step hydrolysates sequentially incubated with Alcalase and other enzymes. Alcalase-treated hydrolysates was similar in proximate composition and Hunter color value, while high in free amino acid content compared with crab cooking drip. Total amino acid content of Alcalase-treated hydrolysates was 11.9 g/100 mL and the major amino acids were glutamic acid (10.2%), proline (10.1%) and glycine (10.7%).

붉은 대게 가공부산물로 이용도가 낮은 자숙액을 이용하여 효소 가수분해물의 제조를 시도하였고, 아울러 이의 맛, 영양 및 건강기능에 대한 특성을 조사하였다. 붉은 대게 자숙액은 일반성분 및 안전성면에서 식품가공소재로 이용하여도 아무런 문제가 없었다. 1단 가수분해물의 경우 TCA soluble index(TSI), ACE 저해능 및 항산화능을 고려하는 경우 Alcalase로 2.0시간 반응시켜 제조하는 것이 가장 적절하였다. 붉은 대게 자숙액 유래 Alcalase 1단 가수분해물을 기질로 하여 Flavourzyme, Neutrase 및 Protamex로 각각 2시간씩 연속적으로 가수분해시킨 2단 가수분해물의 ACE 저해능 및 항산화능은 1단 가수분해물에 비하여 유사하거나 약간 우수하여 2단 가수분해의 필요성이 인정되지 않았다. 붉은 대게 자숙액에 비하여 이를 Alcalase 처리한 가수분해물의 경우 일반성분 및 색조는 차이가 없었으나, glutamic acid 및 aspartic acid와 같은 맛에 관여하는 유리아미노산의 경우 증가하였다. 붉은 대게 유래 Alcalase 처리 가수분해물의 아미노산 함량은 11,859.6 mg/100 mL이었고, 주요 아미노산으로는 glutamic acid(10.2%), proline(10.1%) 및 glycine (10.7%) 등이었다.

Keywords

References

  1. Kim JS, Yeum DM, Kang HG, Kim IS, Kim CS, Lee TG, Heu MS. 2002. Fundamentals and Applications for Canned Foods. 2nd ed. Hyoil Publishing Co., Seoul. p 49-57
  2. Ministry of Maritime Affairs and Fisheries. 2006. http://fs.fips.go.kr/main.jsp
  3. Park YH, Kim SB, Chang DS. 1995. Seafood Processing and Utilization. Hyungsul Publish Co., Seoul. p 201-207, 317-320
  4. Jim JS, Heu MS, Yeum DM. 2001. Component characteristics of canned oyster processing waste water as a food resource. J Korean Soc Food Sci Nutr 30: 299-396
  5. Hayashi T, Yamaguchi K, Konosu S. 1981. Sensory analysis of taste-active components in the extract of boiled snow crab meat. J Food Sci 46: 479-483 https://doi.org/10.1111/j.1365-2621.1981.tb04890.x
  6. Cha YJ, Baek HH. 1995. Quantitative analysis of alkylpyrazines in snow crab cooker effluents. J Korean Soc Food Nutr 24: 454-458
  7. Chang DS, Cho HR, Goo HY, Choe WK. 1989. A development of food preservative with the waste of crab processing. Bull Korean Fish Soc 22: 70-78
  8. Lauer BH, Murray MC, Anderson WE, Guptill EB. 1974. Atlantic queen crab (Chionoecetes opilio), jonah crab (Canncer borealis), and red crab (Geryon quinquedens). Proximate composition of crabmeat from edible tissues and concentration of some major minerals constituents in the ash. J Food Sci 39: 383-385 https://doi.org/10.1111/j.1365-2621.1974.tb02900.x
  9. Krzeczkowski RA, Stone FE. 1979. Amino acid, fatty acid and proximate composition of snow crab (Chinoecetes bairdi). J Food Sci 39: 386-388 https://doi.org/10.1111/j.1365-2621.1974.tb02901.x
  10. AOAC. 1995. Official Methods of Analysis. 16th ed. Association of Official Analytical Chemists, Washington DC. p 69-74
  11. Ministry of Social Welfare of Japan. 1960. Volatile basic nitrogen. In Guide to Experiment of Sanitary Infection. Kenpakusha, Tokyo, Japan. Vol III, p 30-32
  12. Tsutagawa Y, Hosogai Y, Kawai H. 1994. Comparison of mineral and phosphorus contents of muscle and bone in the wild and cultured horse mackerel. J Food Hyg Soc Japan 34: 315-318
  13. KDFA. 2006. 2006 Food Code of the Korean Food and Drug Administration. Moon-young Publishing Co., Seoul. p 70-72, 162
  14. Horiuchi M, Fujimura KI, Terashima T, Iso T. 1982. Method for determination of angiotensin-converting enzyme activity in blood tissue by high-performance liquid chromatography. J Chromatogr 233: 123-130 https://doi.org/10.1016/S0378-4347(00)81738-7
  15. Budincevic M, Vrbaski Z. 1995. Antioxidant activity of Oenothera bienis L. Fat Sci Technol 97: 277-280
  16. Rosen GM, Rauckman EJ. 1984. Spin trapping of superoxide and hydroxyl radicals. In Methods in Enzymology. Academic Press, Orlando, FL. Vol 105, p 189-209
  17. Hiramoto K, Johkoh H, Sako KI, Kikugawa K. 1993. DNA breaking activity of the carbon-centered radical generated from 2,2-azobis(2 amidinopropane) hydrochloride (AAPH). Free Radic Res Commun 19: 323-332 https://doi.org/10.3109/10715769309056521
  18. Nanjo F, Goto K, Seto R, Susuki M, Sakai M, Hara Y. 1996. Scavenging effects of tea catechins and their derivatives on 1,1-diphenyl-2-picrylhlhydrazyl radical. Free Radic Biol Med 21: 895-902 https://doi.org/10.1016/0891-5849(96)00237-7
  19. Cha YT, Kim H, Jang SM, Park JY. 1999. Identification of aroma-active compounds in Korean salt-fermented fishes by aroma extract dilution analysis. 1. Aroma-active compounds in salt-fermented anchovy on the market. J Korean Soc Food Sci Nutr 28: 312-318
  20. Cha YT, Kim H, Park JY. 1999. Identification of aroma-active compounds in Korean salt-fermented fishes by aroma extract dilution analysis. 2. Aroma-active compounds in salt-fermented shrimp on the market. J Korean Soc Food Sci Nutr 28: 319-325
  21. Kato H, Rhue MR, Nishimura T. 1989. Role of acids and peptides in food taste. In Flavor Chemistry: Trends and Development. American Chemical Society, Washington DC. p 158-174
  22. Steel RGD, Torrie H. 1980. Principle and Procedures of Statistics. 1st ed. McGraw-Hill Kogakusha, Tokyo. p 187-221
  23. Yeum DM, Roh SB, Lee TG, Kim SB, Park YH. 1993. Angiotensin-1 converting enzyme inhibitory activity of enzymatic hydrolysates of food proteins. J Korean Soc Food Nutr 22: 226-233
  24. Kim SK, Byun HG, Park PJ, Shahidi F. 2001. Angiotensin I converting enzyme inhibitory peptides purified from bovine skin gelatin hydrolysate. J Agric Food Chem 49: 2992-2997 https://doi.org/10.1021/jf001119u
  25. Halliwell B, Gutteridge JMC. 1989. Free Radicals in Biology and Medicine. 3rd ed. Clarendon Press, Oxford. p 1-20
  26. Cacciuttolo MA, Trinh L, Lumpkin JA, Rao G. 1993. Hyperoxia induces DNA damage in mammalian cell. Free Radic Biol Med 14: 267-276 https://doi.org/10.1016/0891-5849(93)90023-N
  27. Tanaka M, Kuie CW, Nagashima Y, Taguchi T. 1988. Application of antioxidantive maillard reaction products from histidine and glucose to sardine products. Nippon Suisan Gakkaishi 54: 1409-1414 https://doi.org/10.2331/suisan.54.1409
  28. Cho SY, Joo DS, Park SH, Kang HJ, Jeon JK. 2000. Change of taurine content in squid meat during squid processing and taurine content in the squid processing waste water. J Korean Fish 33: 51-54
  29. Mochizuki H, Oda H, Yokogoshi H. 1998. Increasing effect of dietary taurine on the serum HDL-cholesterol concentration in rats. Biosci Biotechnol Biochem 62: 578-579 https://doi.org/10.1271/bbb.62.578
  30. Kim JS, Kim HS, Heu MS. 2006. Mordern Food Science. Hyoil Publishing Co., Seoul. p 42-45
  31. The Korean Nutrition Society. 2005. Dietary Reference Intakes for Koreans. Seoul. p 199-248

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