YAKHAK HOEJI (약학회지)

The Pharmaceutical Society of Korea (대한약학회)
권호 표지

Effects of an Antimicrobial Substance from Bombycis corpus on Antibiotic Resistant Microbes

백강잠으로부터 분리한 항균물질의 항생제 내성균에 대한 효과

  • Lee, Hyeon-Woo (Department of Biochemistry, Yonsei University Wonju College of Medicine, Eurim Biotec Co.) ;
  • Um, Jeong-Sun (Department of Oriental Medical Food & Nutrition, Semyung University) ;
  • Ko, Mi-Kyung (Department of Oriental Medical Food & Nutrition, Semyung University) ;
  • Kim, Mi-Kyung (Department of Oriental Medical Food & Nutrition, Semyung University) ;
  • Eun, Jae-Soon (College of Pharmacy, Woosuk University) ;
  • Jeon, Hoon (College of Pharmacy, Woosuk University) ;
  • Leem, Jae-Yoon (Department of Oriental Medical Food & Nutrition, Semyung University, College of Pharmacy, Woosuk University)
  • 이현우 (연세대학교 원주의과대학, 의림 바이오텍) ;
  • 엄정선 (세명대학교 한방식품영양학과) ;
  • 고미경 (세명대학교 한방식품영양학과) ;
  • 김미성 (세명대학교 한방식품영양학과) ;
  • 은재순 (우석대학교 약학대학) ;
  • 전훈 (우석대학교 약학대학) ;
  • 임재윤 (세명대학교 한방식품영양학과, 우석대학교 약학대학)
  • Published : 2007.08.31
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Abstract

Bombycis corpus, a batryticated silkworm and white-stiff silkworm, is an oriental drug consisting of the dried larva of silkworm, dead and stiffened due to the infection of Beauveria. An peptidyl antimicrobial molecule was purified from B. corpus by reverse phase-column chromatography and HPLC. Its molecular weight was determined to be 2295.45 by using matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry. Its antimicrobial activity was diminished by trypsin digestion. It exhibited a broad antimicrobial spectrum against not only Gram-negative, but also Gram- positive bacteria. Furthermore, it was found to have an antimicrobial activity against vancomycin-resistant enterococci (VRE), methicillin-resistant S. arureus (MRSA), and vancomycin-intermediate resistant S. arureus (VISA). It may be a useful molecule for a new antibiotic development, especially against antibiotic resistant microbe. This substance may play a role in the defense system of this animal against Beauveria bassiana. This is the first report of a peptidyl antimicrobial substance from B. corpus.

Keywords

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