Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Effect of Some Herbal Plant Extracts on the Activation of Dendritic Cells

일부 한약재의 수지상세포 활성화 효과

  • Kim, Do-Soon (Department of Biology, College of Natural Science, Sunchon National University) ;
  • Park, Jung-Eun (Department of Biology, College of Natural Science, Sunchon National University) ;
  • Cho, Hyun-Wook (Department of Biology, College of Natural Science, Sunchon National University) ;
  • Joo, Woo-Hong (Department of Biology, College of Natural Science, Changwon National University) ;
  • Yee, Sung-Tae (Department of Biology, College of Natural Science, Sunchon National University)
  • Published : 2007.03.30
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Abstract

Dendritic cells (DCs) play a pivotal roles in the initiation of T cell-mediated immune responses, making them an attractive in immuno vaccines. Angelica gigas and Cnidium officinale were a medicinal herb widely used in Asian countries. In this study, we examined the effects of A. gigas and C. officinale extracts on the DCs functional maturation and phono-type. Immature DCs were cultured in the presence of GM-CSF and IL-4, and the generated immature DCs were stimulated with OVA in the presence or absence A. gigas and C. officinale extracts, respectively, for 24 hours. The antigen-presenting capacity of A. gigas and C. officinale extracts-treated DCs as analyzed by $CD4^+$ helper T cell clone (OVA-specific) proliferation and cytokines (IL-2 and $IFN-{\gamma}$) production were significantly increased. But A. gigas and C. officinale extracts were not directly effected $CD4^+$ helper T cell clone function. Also, the expression of surface co-stimulatory molecules, including major histocompatibility complex (MHC) class II, CD86 and CD11c, is increased on DCs that were stimulated with A. gigas and C. officinale extracts. These results indicate the immunomodulatory properties of A. gigas and C. officinale extracts, which might be medical supplies or health foods.

본 실험에서는 일부 한약재를 이용하여 수지상세포의 활성화에 미치는 영향을 알아보았다. 그 결과, 실험에 사용한 여섯 가지 한약재 중 선별된 천궁과 당귀는 수지상세포의 항원 제시 능력에 영향을 미쳐, T 세포 증식 반응을 증가시켰고, IL-2와 IFN-r의 분비를 증가시키는 것으로 나타났다. 또한 이러한 T 세포의 활성은 수지상세포의 세포표면 단백질인 MHC classII와 CD86, 그리고 CD11c의 발현 증가에 의한 것임을 확인 할 수 있었다. 이상의 실험 결과, 본 실험에서 선별된 천궁과 당귀는 수지상세포를 활성화시키는 효과가 있는 것으로 생각된다.

Keywords

References

  1. Avigan, D. 1999. Dendritic cells: development, function and potential use for cancer immunotherapy. Blood reviews 13, 51-64 https://doi.org/10.1016/S0268-960X(99)90023-1
  2. Banchereau, J., F. Briere, C. Caux, J. Davoust, S. Lebecque, Y. J. Liu, B. Pulendran and K. Palucka. 2000. Immunobiology of dendritic cells. Annu. Rev. Immunol. 18, 767-811 https://doi.org/10.1146/annurev.immunol.18.1.767
  3. Banchereau, J. and R. M. Steinman. 1998. Dendritic cells and the control of immunity. Nature 392, 245-52 https://doi.org/10.1038/32588
  4. Bell, D., J. W. Young and J. Banchereau. 1999. Dendritic cells. Adv. Immunol. 72, 255-232 https://doi.org/10.1016/S0065-2776(08)60023-1
  5. Fearnley, D. B., L. F. Whyte, S. A. Carnoutsos, A. H. Cook and D. N. J. Hart. 1999. Monitoring human blood dendritic cell numbers in normal individuals and in stem cell transplantation. Blood 93: 728-36
  6. Hart, D. N. 1997. Dendritic cells: unique leukocyte populations which control the primary immune response. Blood 90, 3245-3287
  7. Inaba, K., M. Witmer-Pack, M. Inaba, K. S. Hathcock, H. Sakuta, M. Azuma, H. Yagita, K. Okumura, P. S. Linsley, S. Ikehara, S. Muramatsu, R. J. Hodes and R. M. Steinman. 1994. The tissue distribution of the B7-2 costimulator in mice: Abundant expression on dendritic cells in situ and during maturation in vivo. J. Exp. Med. 180, 1849-1860 https://doi.org/10.1084/jem.180.5.1849
  8. K. Inaba, M. Inaba, N. Romani, H. Aya, M. Deguchi, S. Ikehara, S. Muramatsu and R. M. Steinman. 1992. Generation of large numbers of dendritic cells from mouse bone marrow cultures supplemented with granulocyte/macrophage colony-stimulating factor. J. Exp. Med. 176, 1693-1702 https://doi.org/10.1084/jem.176.6.1693
  9. Jacques B. and M. Ralph. 1998. Dendritic cells and the control of immunity. Nature 392, 245-252 https://doi.org/10.1038/32588
  10. Jain, J., C. Loh and A. Rao. 1995. Transcription regulation of the IL-2 gene. Curr. Opin. Immunol. 7, 333-342 https://doi.org/10.1016/0952-7915(95)80107-3
  11. T. Kikuchi, N. Ohno and T. Ohno. 2002. Maturation of dendritic cells induced by Candida beta-D-glucan. International Immunopharmacology 2, 1503-1508 https://doi.org/10.1016/S1567-5769(02)00084-X
  12. E. S. Kim, C. S. Kim, M. H. Li, H. S. Bom, J. J. Min, H, J. Jeong, S. M. Kim, H. C. Song and J. J. Lee. 2003. Radioresistance of Dendritic Cells. Korean J. Nucl. Med. 9, 190-195
  13. Kim, G. Y., M. Y. Lee, H. J. Lee, D. O. Moon, C. M. Lee, C. Y. Jin, Y. H. Choi, Y. K. Jeong, K. T. Chung, J. Y. Lee, I. H. Choi and Y. M. Park. 2005. Effect of water-soluble proteoglycan isolated from Agaricus blazei on the maturation of murine bone marrow-derived dendritic cells. International Immunopharmacology 5, 1523-1532 https://doi.org/10.1016/j.intimp.2005.02.018
  14. Lee, J. K., M. K. Lee, Y. P. Yun, Y. S. Kim, J. S. Kim, Y. S. Kim, K. J. Kim, S. S. Han and C. K. Lee. 2001. Acemannan purified from Aloe vera induces phenotypic and functional maturation of immature dendritic cells. International Inmumopharmacology 1, 1275-1284 https://doi.org/10.1016/S1567-5769(01)00052-2
  15. Minami, Y., T. Kono, T. Miyazaki and T. Taniguchi. 1993. The IL-2 receptor complex: its structure, function, and target genes. Annu. Rev. Immunol. 11, 245-267 https://doi.org/10.1146/annurev.iy.11.040193.001333
  16. Morrison, L. A., A. E. Lukacher, V. L. Braciale, D. P. Fan and T. J. Braciale. 1986. Differences in antigen presentation to MHC class I -and MHC class II -restricted influenza virus-specific cytolytic T-lymphocyte clones. J. Exp. Med. 163, 903-910 https://doi.org/10.1084/jem.163.4.903
  17. Nabeshima, S., M. Murata, M. Hamada, Y. Chong, K. Yamaji and J. Hayashi. 2004. Maturation of monocyte-derived dendritic cells by Hochu-ekki-to, a traditional Japanese herbal medicine. International Immunopharmacology 4, 37-45 https://doi.org/10.1016/j.intimp.2003.10.002
  18. Rattis, F. M., J. Peguet-Navarro, M. J. Staquet, C. Dezutter-Dambuyant, P. Courtellemont, G. Redziniac and D. Schmitt. 1996. Expression and function of B7-1 (CD80) and B7-2 (CD86) on human epidermal Langerhans cells. Eur. J. Immunol. 26, 449-453 https://doi.org/10.1002/eji.1830260227
  19. Steimle, V., C. A. Siegrist, A. Mottet, B. Lisowska-Grospierre and B. Mach. 1994. Regulation of MHC class II expression by interferon-${\gamma}$ mediated by the trans-activator gene CllTA. Science 265, 106-109 https://doi.org/10.1126/science.8016643
  20. Takei, M., E. Tachikawa, H. Hasegawa and J. J. Lee. 2004. Dendritic cells maturation promoted by M1 and M4, end products of steroidal ginseng saponins metabolized in digestive tracts, drive a potent Th1 polarization. Biochemical Pharmacology 68, 441-452 https://doi.org/10.1016/j.bcp.2004.04.015
  21. Tarte, K. and B. Klein. 1999. Dendritic cell-based vaccine: a promising approach for cancer immunotherapy. Leukemia 13, 653-63 https://doi.org/10.1038/sj/leu/2401394
  22. Shin, M. K. 1997. Clinical Traditional Herbalogy. 2nd eds., Yeong Lim's Publisher. Seoul, Korea