생명과학회지 (Journal of Life Science)

한국생명과학회 (Korean Society of Life Science)
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ErbB2의 kinase 영역이 β-catenin과 ErbB2의 결합에 필요하다

ErbB2 kinase domain is required for ErbB2 association with β-catenin

  • 하남출 (부산대학교 약학대학 신약개발연구소) ;
  • 슈완핑 (미국 국립암연구소 비뇨종양학실) ;
  • 넥컬즈렌 (미국 국립암연구소 비뇨종양학실) ;
  • 정연진 (부산대학교 약학대학 신약개발연구소)
  • Ha, Nam-Chul (Research Institute for Drug Development, College of Pharmacy, Pusan National University) ;
  • Xu, Wanping (Urologic Oncology Branch, National Cancer Institute) ;
  • Neckers, Len (Urologic Oncology Branch, National Cancer Institute) ;
  • Jung, Yun-Jin (Research Institute for Drug Development, College of Pharmacy, Pusan National University)
  • 발행 : 2007.03.30
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초록

${\beta}-catenin$과 결합하는 ErbB2의 부위를 조사하기 위하여 proteasome에 의하여 분해되지 않는 ${\beta}-catenin$과 다양한 ErbB2 construct를 COS7 세포에 transfection한 후 ErbB2 단백질을 그것의 항체로 가라앉혔다. 이 때 공침한 ${\beta}-catenin$을 Western blot으로 분석하였다. C 말단에서부터 잘려진 ErbB2 단백질 중에 kinase 영역을 가지고 있는 것들만 ${\beta}-catenin$과 공침하였다. kinase 영역의 필요성을 확인하기 위하여 kinase 영역이 내부에서 제거된 ErbB2 construct를 ${\beta}-catenin$과 transfection 한 후 동일한 실험을 실시하였다. 이 실험에서 ${\beta}-catenin$는 kinase 영역이 내부적으로 제거된 ErbB2 단백질과 공침하지 않았다. 이 결과는 ${\beta}-catenin$과 결합하는 ErbB2의 위치는 kinase 영역내에 있음을 제시한다.

To investigate the region of ErbB2 for the $ErbB2-{\beta}-catenin$ interaction, a proteasome $resistant-{\beta}-catenin$ and various ErbB2 constructs were transfected in COS7 cells. ErbB2 proteins were immunoprecipitated, and coimmunoprecipitated ${\beta}-catenin$ was examined by Western blotting. ${\beta}-catenin$ coimmunoprecipitated with full length ErbB2. Of the truncated ErbB2 proteins DT (1-1123), DHC (1-1031) and DK (1-750), the ErbB2 constructs containing the kinase domain, DT and DHC, precipitated together with ${\beta}-catenin$ but DK containing no kinase domain did not. To further test the requirement of the kinase domain for ${\beta}-catenin-ErbB2$ interaction, the presence of ${\beta}-catenin$ in the immunocomplex was examined following transfection with an ErbB2 mutant (${\triangle}750-971$) whose kinase domain is internally deleted and subsequent immunoprecipitation of the ErbB2 mutant. ${\beta}-catenin$ was not detected in the immunocomplex. These results suggest that the ErbB2 kinase domain comprises a potential site for ${\beta}-catenin$ binding to the receptor tyrosine kinase.

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