생명과학회지 (Journal of Life Science)

한국생명과학회 (Korean Society of Life Science)
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알칼리성 소독액에 의한 조류인플루엔자바이러스 불활성화

Inactivation of Avian Influenza Viruses by Alkaline Disinfectant Solution

  • 조수경 (충남대학교 수의과대학 인플루엔자연구 실험실) ;
  • 김희만 (충남대학교 수의과대학 인플루엔자연구 실험실) ;
  • 이창준 (충남대학교 수의과대학 인플루엔자연구 실험실) ;
  • 이주섭 (충남대학교 수의과대학 인플루엔자연구 실험실) ;
  • 서상희 (충남대학교 수의과대학 인플루엔자연구 실험실)
  • Jo, Su-Kyung (Laboratory of Influenza Research, College of Veterinary Medicine, Chungnam National University) ;
  • Kim, Heui-Man (Laboratory of Influenza Research, College of Veterinary Medicine, Chungnam National University) ;
  • Lee, Chang-Jun (Laboratory of Influenza Research, College of Veterinary Medicine, Chungnam National University) ;
  • Lee, Joo-Seob (Laboratory of Influenza Research, College of Veterinary Medicine, Chungnam National University) ;
  • Seo, Sang-Heui (Laboratory of Influenza Research, College of Veterinary Medicine, Chungnam National University)
  • 발행 : 2007.03.30
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초록

조류인플루엔자바이러스는 사람 및 동물에 상당한 위협을 가하고 있다. 이 연구는 알칼리성 소독용액이 H5N1, H3N2, H6N1, H9N2형 조류인플루엔자바이러스를 불활성화시킬 수 있는지를 조사했다. 알칼리성 소독용액을 생리식염수에 100배 희석한 경우 조류인플루엔자가 MDCK 세포에서 증식하는 것을 완전히 억압하였다. 형광항체법을 이용한 실험에서도 알칼리성소독액을 처리한 H9N2 조류인플루엔자바이러스는 MDCK세포에서 증식이 억제되었고, 알칼리성 소독액을 처리하지 않은 H9N2조류인플루엔자바이러스는 증식이 억제되지 않았다. 이 결과는 알칼리성 소독액은 고병원성 H5N1을 포함을 조류인플루엔자바이러스를 방역하는데 도움이 될 수 있음을 시사한다.

Avian influenza viruses cause a considerable threat to humans and animals. In this study, we investigated whether alkaline disinfectant solution can inactivate H5N1, H3N2, H6N1, and H9N2 subtypes of avian influenza virus. When H5N1, H3N2, H6N1, and H9N2 avian influenza viruses were treated with alkaline solution diluted with PBS (pH 7.2) prior to infection into MDCK cells, alkaline disinfectant solution (at dilutions up to $10^{-2}$) completely inactivated all avian influenza subtypes tested. To confirm the inactivation of avian influenza viruses by alkaline disinfectant solution, we used an immunofluorescence assay with influenza A anti-nucleoprotein antibody and FITC-labeled secondary antibody to stain MDCK cells infected with avian H9N2 influenza viruses. No staining was observed in MDCK rells infected with H9N2 viruses that were pre-treated with a $10^{-2}$ dilution of alkaline disinfectant solution, while strong staining was observed in MDCK cells infected with H9N2 viruses without pre-treatment. Our results indicate that alkaline solution could help to control avian influenza viruses including the highly pathogenic H5N1 subtype.

키워드

참고문헌

  1. Areechokchai, D., C. Jiraphongsa, Y. Laosiritaworn, W. Hanshaoworakul and O'Reilly. 2006. Investigation of avian influenza (H5N1) outbreak in humans-Thailand, MMWR. Morb. Marta. Wkly Rep. 55, 3-6
  2. Alexander, D. J. 2000. A review of avian influenza in different bird species. Vet. Microbial. 74, 3-13 https://doi.org/10.1016/S0378-1135(00)00160-7
  3. Banks, J., E. C. Speidel, P. A. Harris and D. J.Alexander. 2000. Phylogenetic analysis of influenza A viruses of H9 haemagglutinin subtype. Avian Pathol. 29, 353-360 https://doi.org/10.1080/03079450050118485
  4. Choi, Y. K., S. H. Seo, J. A. Kim, R. J. Webby and R. G. Webster. 2005. Avian influenza viruses in Korean live poultry markets and their pathogenic potential. Virol. 332, 529-537 https://doi.org/10.1016/j.virol.2004.12.002
  5. Chen, H., G. J. Smith, K. S. Li, J. Wang, X. H. Fan, J. M. Rayner, D. Vijaykrishna, J. X. Zhang, L .J. Zhang, C. T. Guo, C. L. Cheung, K. M. Xu, L. Duan, K. Huang, K. Qin, Y. H. Leung, W. L. Wu, H. R. Lu, Y. Chen, N. S. Xia, T. S. Naipospos, K. Y. Yuen, S. S. Hassan, S. Bahri, T. D. Nguyen, R. G. Webster, J. S. Peiris and Y. Guan. 2006. Establishment of multiple sublineages of H5N1 influenza virus in Asia: implications for pandemic control. Proc. Natl. Acad. Sci. U. S. A. 103(8), 2845-50 https://doi.org/10.1073/pnas.0511120103
  6. Darnell, M. E .R., K. Subbarao, S. M. Feinstone and D. R. Taylor. 2004. Inactivation of the coronavirus that induces severe acute respiratory syndrome, SARS-Cov. J. Virol. Methods 121, 85-91 https://doi.org/10.1016/j.jviromet.2004.06.006
  7. Ducatez, M. F., C. M. Olinger, A. A.Owoade, S. W. De Landtsheer, Ammerlaan, H. C. Niesters, A. D. Osterhaus, R. A. Fouchier and C. P. Muller. 2006. Avian flu: multiple introductions of H5N1 in Nigeria, Nature 442(7098), 37 https://doi.org/10.1038/442037a
  8. Fouchier, R. A., V. Munster, A. Wallensten, T. M. Bestebroer, S. Herfst, D. Simith, C. F., Rimmelzwaan, B. Olsen and A. D. Osterhus. 2005. Characterization of a novel influenza A virus hemagglutinin subtype (H16) obtained from black-headed gulls. J. Virol. 79, 2814-2822 https://doi.org/10.1128/JVI.79.5.2814-2822.2005
  9. Guan, Y., K. F. Shortridge, S. Krauss, P. S. Chin, K. C. Dyrting, T. M. Ellis, R. G. Webster and M. Peiris, 2000. H9N2 influenza viruses possessing H5N1-like internal genomes continue to circulate in poultry in southeastern China. J. Virol. 74, 9372-9380 https://doi.org/10.1128/JVI.74.20.9372-9380.2000
  10. Guo, Y. J., S. Krauss, D. A. Senne, J. P. Mo, K. S. La, X. P. Xiong, M. Norwood, K. F. Shortridge, R. G. Webster and Y. Guan. 2000. Characterization of the pathogenicity of members of the newly established H9N2 influenza virus lineages in Asia. Virol. 267, 279-288 https://doi.org/10.1006/viro.1999.0115
  11. Li, K. S., K. M. Xu, J. S. Peiris, L. L. Poon, K. Z. Yu, K.Y. Yuen, K. F. Shortridge, R. G. Webster and Y. Guan. 2003. Characterization of H9 subtype influenza viruses from the ducks of southern China: a candidate for the next influenza pandemic in humans. J. Virol. 77, 6988-6994 https://doi.org/10.1128/JVI.77.12.6988-6994.2003
  12. Lin Y. C. and S. C. Wu. 2006. Effects of ozone exposure on inactivation of intra-and extracellular enterovirus 71. Antiviral Res. 70, 147-153 https://doi.org/10.1016/j.antiviral.2005.12.007
  13. Lin Y. P., M. Shaw, V. Gregory, K. Cameron, W. Lim, A. Klimov, K. Subbarao, Y. Guan, S. Krauss, K. Shortridge, R. G. Webster, N. Cox and A. Hay. 2000. Avian-to-human transmission of H9N2 subtype influenza A viruses: relationship between H9N2 and H5N1 human isolates. Proc. Natl. Acad. Sci. U. S. A. 97, 9654-9658 https://doi.org/10.1073/pnas.160270697
  14. Lu, X., Renshaw, M., T. M. Tumpey, G. D. Kelly, J. Hu-Primmer and J. M. Katz. 2001. Immunity to influenza A H9N2 viruses induced by infection and vaccination. J. Virol. 75, 4896-4901 https://doi.org/10.1128/JVI.75.10.4896-4901.2001
  15. Naeem K., A. Ullah, R. J. Manvell and D. J. Alexander. 1999. Avian influenza A subtype H9N2 in poultry in Pakistan. Vet. Rec. 145:560 https://doi.org/10.1136/vr.145.19.560
  16. Nagayama A. 2006. Inactivation of influenza A virus by gentian violet (GV) and GV -dyed cotton cloth, and bacterial activities of these agents. J. Infect. Chemother. 12, 73-79 https://doi.org/10.1007/s10156-005-0426-7
  17. Nguyen, D. C., T. M. Uyeki, S. Jadhao, T. Maines, M. Shaw, Y. Matsuoka, C. Smith, T. Rowe, X. Lu, H. Hall, X. Xu, A. Balish, A. Klimov, T. M. Tumpey, D. E. Swayne, L. P. T. Huynh, H. K. Nghiem, H. H. T. Nguyen, L. T. Hoang, N. J. Cox and J. M. Katz. 2005. Isolation and characterization of avian influenza viruses, including highly pathogenic H5N1, from poultry in live bird markets in Hanoi, Vietnam, in 2001. J. Virol. 79, 4201-4212 https://doi.org/10.1128/JVI.79.7.4201-4212.2005
  18. Nwachuku, N., C. P. Gerba, A. Oswald and F. D. Mashadi. 2005. Comparative inactivation of adenovirus serotypes by UV light disinfection. Applied and environment microbiology 71, 5633-5636 https://doi.org/10.1128/AEM.71.9.5633-5636.2005
  19. Reed, L. J. and H. Muench. 1938. A simple method of estimating fifty per cent endpoints. Am. J. Hyg. 27, 493-497
  20. Simonet, J. and C. Gantzer. Degradation of the poliovirus 1 genome by chlorine dioxide. 2006. J. Appl. Mcirobiol. 100, 862-870 https://doi.org/10.1111/j.1365-2672.2005.02850.x
  21. Smith, G. J., T. S. Naipospos, T. D. Nguyen, M. D. de Jong, D. Vijaykrishna, T .B. Usman, S. S. Hassan, T. V. Nguyen, T. V. Dao, N. A. Bui, Y. H. Leung, C. L. Cheung, J. M. Rayner, J. X. Zhang, L. J. Zhang, L. L. Poon, K .S. Li, V. C. Nguyen, T. T. Hien, J. Farrar, R. G. Webster, H. Chen, J .S. Peiris and Y. Guan. 2006. Evolution and adaptation of H5N1 influenza virus in avian and human hosts in Indonesia and Vietnam. Virology 350(2), 258-68 https://doi.org/10.1016/j.virol.2006.03.048
  22. Suarez, D. L. and E. Spackman. 2003. The effect of various disinfectants on detection of avian influenza virus by real time RT-PCR. Avian Dis. 47, 1091-1095 https://doi.org/10.1637/0005-2086-47.s3.1091
  23. Zhou, J. Y., H. G. Shen, H. X. Chen, G. Z. Tong, M. Liao, H. C. Yang and J.X. Liu. 2006. Characterization of a highly pathogenic H5N1 influenza virus derived from bar-headed geese in China. J. Gen. Virol. 87, 1823-1833 https://doi.org/10.1099/vir.0.81800-0
  24. Webster, R. G., W. J. Bean, O. T. Gorman, T. M. Chambers and Y. Kawaoka. 1992. Evolution and ecology of influenza A viruses. Microbiol Rev. 56, 152-179

피인용 문헌

  1. Inactivation of influenza A virus H1N1 by disinfection process vol.38, pp.5, 2010, https://doi.org/10.1016/j.ajic.2010.03.003