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Identification of Volatile Compounds of 4 Grape Species by Storage Conditions

전자코와 GC/MS를 이용한 포도 품종별 저장 조건에 따른 휘발성 향기 성분 연구

  • Lee, Yun-Jeung (Dept. of Food Science and Technology, Chungnam National University) ;
  • Lee, Ki-Teak (Dept. of Food Science and Technology, Chungnam National University)
  • Published : 2007.07.30

Abstract

Volatile flavor compounds of 4 grape species (Campbell, Sheridan, Red globe, and Meoru) were identified during 3-day storage at either $4^{\circ}C$ or room temperature. Each sample was analyzed by solid-phase micro-extraction (SPME) method combined with gas chromatography-mass spectrometry. Also electronic nose composed of 12 different metal oxide sensors was used to differentiate flavors of grapes. Sensitivities (delta $R_{gas}/R_{air}$) of sensors from electronic nose were obtained by principal component analysis (PCA). Proportion of the first principal component was 99.30% at $4^{\circ}C$ and 99.36% at room temperature, respectively. In our result, flavor patterns of grape can be differentiated according to the storage period. The major volatile flavor compounds were 1-hexanol, hexanoic acid and its ethyl ester, and phenylethyl alcohol with the presence of butanoic acid and its ethyl ester, acetic acid, benzeneacetic acid and its ethyl ester.

품종에 따른 포도를 이용하여 3일 동안 $4^{\circ}C$와 실온에 각각 저장함에 따라 변화하는 전체적인 향의 패턴을 전자코를 통하여 분석하였고 SPME-GC/MS 분석 결과를 통하여 휘발성분을 분리 동정하였다. 대표적인 포도의 주요 휘발 성분은 alcohols, esters, acids, terpenes 등으로 GC/MS 분석 결과 butanoic acid/ethyl ester와 1-hexanol, hexanoic acid/ethyl ester, phenylethyl alcohol 등이 주요 성분으로 동정되었다. 캠벨은 다른 품종에 비해 다양한 휘발 성분이 동정되었고 레드글로브와 머루 품종에서는 1-hexanol, 2-decene, 8-methyl-(z)/1-dodecanol 등을 제외한 나머지 휘발 성분은 거의 나타나지 않았다. 또한 전자코를 이용하여 휘발성 향기성분 패턴 분석결과 $4^{\circ}C$와 실온 저장한 시료의 제 1주성분 값의 기여율은 각각 99.30, 99.36%로 품종에 따라 구분이 가능하였다. $4^{\circ}C$ 저장 시 저장 기간에 따른 제 1주성분 값의 변화 범위는 실온에서 저장한 것에 비해 적었음을 확인할 수 있었다.

Keywords

References

  1. Kanner J, Frankel E, Granit R, German B, Kinsella JE. 1994. Natural antioxidants in grapes and wines. J Agric Food Chem 42: 64-69 https://doi.org/10.1021/jf00037a010
  2. Vinson JA, Yang J, Proch J, Liang X. 2000. Grape juice, but not orange juice, has in vitro, ex vivo, and in vivo antioxidant properties. J Med Food 3: 167-172 https://doi.org/10.1089/jmf.2000.3.167
  3. The AF News Press. 2002. The Annual Report of Food Industry. Seoul. p 200-205
  4. Lee SY, Lee KH, Chang KS, Lee SK. 2000. The changes of aroma in wine treated with reverse osmosis system. Korean J Food Sci Tech 32: 17-24
  5. Park ER, Kim KS. 2000. Volatile flavor components in various varieties of grape (Vitis vinifera L.). Korean J Postharvest Sci Technol 7: 366-372
  6. Rosillo L, Salinas R, Garijo J, Alonso GL. 1999. Study of volatiles in grapes by dynamic headspace analysis application to the differentiation of some Vitis vinifera varieties. J Chromatography A 847: 155-159 https://doi.org/10.1016/S0021-9673(99)00036-9
  7. Bonino M, Schellino R, Rizzi C, Aigotti R, Delfini C, Baiocchi C. 2003. Aroma compounds of an Italian wine (Ruche) by HS-SPME analysis coupled with GC-ITMS. Food Chemstry 80: 125-133 https://doi.org/10.1016/S0308-8146(02)00340-0
  8. Schreier P, Drawert F, Junker A. 1976. Identification of volatile constituents from grapes. J Agric Food Chem 24: 331-336 https://doi.org/10.1021/jf60204a032
  9. SAS Institute, Inc. 2000. SAS user's guide. Statistical analysis systems institute, Cary, NC, USA
  10. Jang MR, Jeong HJ, Lee HK. 2002. Preparation of optimal condition for residual pesticides analysis by solid-phase microextraction in water. J Korean Soc Water Quality 18: 421-433
  11. Park GB, Lee SG. 2000. Determination of volatile organic compounds (VOCs) in drinking water using solid phase microextraction (SPME). Anal Sci Technol 13: 277-281
  12. Kataoka H, Lord HL, Pawliszyn J. 2000. Applications of solid-phase microextraction in food analysis. J Chromatography A 880: 35-62 https://doi.org/10.1016/S0021-9673(00)00309-5
  13. Kotseridis Y, Baumes R. 2000. Identification of impact odorants in bordeaux red grape juice, in the commercial yeast used for its fermentation, and in the produced wine. J Agric Food Chem 48: 400-406 https://doi.org/10.1021/jf990565i
  14. Ferreira V, Hernandez-Orte P, Escudero A, Lopez R, Cacho J. 1999. Semipreparative reversed-phase liquid chromatographic fractionation of aroma extracts from wine and other alcoholic beverages. J Chromatography A 864: 77-78 https://doi.org/10.1016/S0021-9673(99)01004-3
  15. Shin JA, Lee KT. 2003. The identification of blended sesame oils by electronic nose. Korean J Food Sci Tech 35: 648-652

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  1. 대사체분석 기술을 이용한 들깨 종자의 품질 특성 및 항산화 효과 vol.51, pp.3, 2007, https://doi.org/10.9721/kjfst.2019.51.3.193