Journal of Veterinary Clinics (한국임상수의학회지)

The Korean Society of Veterinary Clinics (한국임상수의학회)
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Evaluation of the Canine Stifle Joint after Transection of the Cranial Cruciate Ligament and Medial Collateral Ligament, and Medial Meniscectomy without Postoperative Exercise

앞십자인대 및 내측 곁인대 절제와 내측 반월판 절제술을 한 뒤 수술후 운동을 실시하지 않은 개의 무릎 관절의 평가

  • Lee, Hae-Beom (College of Veterinary Medicine, Chonbuk National University) ;
  • Jeong, Chang-Woo (College of Veterinary Medicine, Chonbuk National University) ;
  • Kim, Nam-Soo (College of Veterinary Medicine, Chonbuk National University)
  • Published : 2007.09.30
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Abstract

This study was to determine whether canine model which produce acute permanent joint instability in short period without postoperative exercise have a degenerative changes and also evaluated its suitability as an appropriate animal OA models. Ten skeletally mature beagle dogs underwent a unilateral surgical transection of the cranial cruciate ligament and, the medial collateral ligament as well as a medial meniscectomy. The contra-lateral joint was used as control. After 12 weeks, After 12 weeks, the amount of joint damage, inflammation and biochemical change of synovial fluid was evaluated. Histological analysis showed chondrocyte clone formation, hypertrophy of the cartilage and moderate loss of proteoglycans in the experimental joints compared to control joints. In addition, the synovial inflammation in the experimental joints was observed. Biochemical analysis of SF showed significantly increased MMP (matrix metalloproteinase) -2 and -9 in experimental joints compared to control joints. This canine OA model shows the characteristics of degenerative joint disease, and may have a advantages of reducing the time and cost because postoperative exercise is not needed in this OA model.

본 연구는 개에서 수술적으로 짧은 기간 동안 관절의 불안정성을 최대로 유도하여 수술후 운동 없이 관절의 퇴행성 변화의 유무 및 정도와 골관절염 모델로 적합한지를 평가 하였다. 10두의 비글견을 본 실험에 사용하였다. 실험군은 오른쪽 무릎관절의 앞십자인대 및 내측 곁인대의 절제와 내측 반월 연골 절제술을 시행하였으며, 왼쪽 무릎관절을 대조군으로 사용하였다. 수술 12주 후 연골의 손상, 윤활막의 염증 정도와 윤활액의 생화학적 변화를 평가 하였다. 조직 검사에서 연골의 비후, 연골 세포의 분화, 프로테오글리칸의 소실 및 윤활막의 염증이 대조군과 비교하여 유의성 있는 증가가 관찰되었다. 윤활액의 생화학적 검사에서 유의성 있게 증가된 MMP-2, -9의 농도를 실험군에서 관찰 하였다. 이상의 결과로부터 본 연구에 수행한 관절염 모델은 골관절염 유도가 가능하며, 술후 운동으로 소모되는 시간 및 비용을 줄 일수 있는 장점 있어 골관절염 모델로서 유용하게 활용될 것으로 판단된다.

Keywords

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