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Methods for Evaluation of Antioxidant Activity: Application to Taurine

항산화 활성의 평가를 위한 연구법: 타우린의 적용

  • Kim, Bong-Hee (College of Pharmacy and Research Center for Transenic Cloned Pigs, Chungnam National University) ;
  • Oh, Jung-Min (College of Pharmacy and Research Center for Transenic Cloned Pigs, Chungnam National University) ;
  • Yun, Kang-Uk (College of Pharmacy and Research Center for Transenic Cloned Pigs, Chungnam National University) ;
  • Kim, Chung-Hyeon (College of Pharmacy and Research Center for Transenic Cloned Pigs, Chungnam National University) ;
  • Kim, Sang-Kyum (College of Pharmacy and Research Center for Transenic Cloned Pigs, Chungnam National University)
  • 김봉희 (충남대학교 약학대학, 충남대학교 형질전환 복제돼지센터) ;
  • 오정민 (충남대학교 약학대학, 충남대학교 형질전환 복제돼지센터) ;
  • 윤강욱 (충남대학교 약학대학, 충남대학교 형질전환 복제돼지센터) ;
  • 김충현 (충남대학교 약학대학, 충남대학교 형질전환 복제돼지센터) ;
  • 김상겸 (충남대학교 약학대학, 충남대학교 형질전환 복제돼지센터)
  • Published : 2007.09.30

Abstract

Although taurine (2-aminoethanesulfonic acid) can inhibit oxidative stress in both animal and epidemiological studies, it is obscure whether taurine directly scavenges oxy-radicals or indirectly regulates oxidant production and/or antioxidant defense system. The reason for this discrepancy remains unknown but may be due, in part, to the lack of a validated assay system for evaluating oxy-radical scavenging capacity. The antioxidant activities of taurine and hypotaurine (2-aminoethanesulfinic acid), a precursor of taurine, against peroxyl radicals, hydroxyl radicals and peroxynitrites were determined by the total oxy-radical scavenging capacity (TOSC) assay and cell-based assay using H4IIE cells. tert-Butylhydroperoxide or hydrogen peroxide-induced cell toxicity determined by MTT assay was markedly inhibited by 10mM taurine or hypotaurine. The tert-butylhydroperoxide- or hydrogen peroxide-induced changes in oxidative stress markers, such as cellular glutathione and malondialdehyde, were ameliorated by 10mM taurine or hypotaurine. However, specific TOSC values calculated from the slope of the linear regression for taurine against peroxyl radicals, hydroxyl radicals or peroxynitrites were all less than 1 TOSC/mM. On the other hand specific TOSC values for hypotaurine against peroxyl radicals, hydroxyl radicals or peroxynitrites were 48, 2096, or 69 TOSC/mM, respectively. These results suggest that taurine protects cells against oxidative insults, which is not ascribed to directly scavenging activity of taurine against oxy-radicals. These results support the idea that the oxidation state of sulfur in antioxidants may be a determinant of oxy-radical scavenging capacity.

Keywords

References

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