Reproductive and Developmental Biology

  • 제31권3호
  • /
  • Pages.133-137
  • /
  • 2007
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  • 1738-2432(pISSN)
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  • 2288-0151(eISSN)
한국동물번식학회 (The Korean Society of Animal Reproduction)
권호 표지

돼지 정자내 Phospholipids Hydroperoxide Glutathione Peroxidase와 Heparin-Binding Protein의 발현 수준과 번식 능력의 관계

Relationship between the mRNA Expression Levels of Sperm Phospholipids Hydroperoxide Glutathione Peroxidase and Heparin-Binding Protein, and in-Vivo Fertility in Boars

  • 오신애 (중앙대학교 산업과학대학 동물자원과학과) ;
  • 신차균 (중앙대학교 산업과학대학 생명공학과) ;
  • 유영아 (중앙대학교 산업과학대학 동물자원과학과) ;
  • 한경수 (중앙대학교 산업과학대학 동물자원과학과) ;
  • 방명걸 (중앙대학교 산업과학대학 동물자원과학과)
  • Oh, Shin-Ae (Department of Animal Science and Technology, Chung-Ang University) ;
  • Shin, Cha-Gyun (Department of Biotechnology, Chung-Ang University) ;
  • You, Young-Ah (Department of Animal Science and Technology, Chung-Ang University) ;
  • Han, Kyung-Soo (Department of Animal Science and Technology, Chung-Ang University) ;
  • Pang, Myung-Geol (Department of Animal Science and Technology, Chung-Ang University)
  • 발행 : 2007.09.30
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초록

본 연구는 정자 내의 phsopholipid hydroxide glutathion peroxidase (PHGPx) mRNA 발현 수준, heparin-binding protein (HBP) mRNA 발현 수준, 수태율 그리고 산자수 사이의 관계를 조사하고자 실시하였다. 수태율과 산자수 사이에 있어서 상관관계는 나타나지 않았다. PHGPx mRNA 발현 수준에 있어 산자수가 10두 이상 군에서 $(2,414.7{\pm}400.7)$ 8두미만 군보다 $(1,875.8{\pm}311.2)$ 높게 나타났으나 유의적인 차이는 없었다. HBP mRNA 발현 수준에 있어서도 산자수 10두 이상 군에서 $(2,255.9{\pm}360.8)$ 8두 미만 군보다 $(2,155.4{\pm}378.0)$ 약간 높은 결과를 보였으나, 유의적인 차이는 인정되지 않았다. PHGPx mRNA 발현 수준과 산자수 사이의 관계는 (r=0.206) 정의 상관관계를 보였으나, 유의한 상관관계는 나타나지 않았다. 본 실험의 결과, PHGPx와 HBP의 발현수준이 수태율, 산자수와 유의한 상관관계를 보이지 않았기 때문에 PHGPx와 HBP가 정자의 수정능력을 예측하기에는 미흡한 면이 있다.

The objective of the study was to determine the relationship between the mRNA expression levels of sperm phospholipids hydroperoxide glutathione peroxidase (PHGPx) and heparin-binding protein (HBP), and in-vivo fertility in boars. The farrowing rate was not correlated with litter size. Sperm PHGPx mRNA expression level of the larger litter size (over 10) group $(2,414.7{\pm}400.7)$ was high that of smaller litter size (below 8) group $(1,875.8{\pm}311.2)$. Sperm HBP mRNA expression level was also higher in the larger litter size $(2,255.9{\pm}360.8)$ group than the smaller litter size $(2,155.4{\pm}378.0)$. However, significant differences were not observed. Sperm PHGPx mRNA level was correlated positively with litter size (r=0.206). Because the expression levels of PHGPx and HBP are not strongly correlated with in-vivo fertility, PHGPx and HBP can not be considered a predictive measure for fertility in boars.

키워드

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