Parasites, Hosts and Diseases

The Korea Society for Parasitology and Tropical Medicine (대한기생충학·열대의학회)
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Metacercarial proteins interacting with WD40-repeat protein of Clonorchis sinensis

  • Cho, Pyo-Yun (Department of Parasitology, Chung-Ang University College of Medicine) ;
  • Kim, Tae-Im (Department of Parasitology, Chung-Ang University College of Medicine) ;
  • Li, Shunyu (Department of Parasitology, Chung-Ang University College of Medicine) ;
  • Hong, Sung-Jong (Department of Parasitology, Chung-Ang University College of Medicine) ;
  • Choi, Min-Ho (Department of Parasitology and Tropical Medicine, Seoul National University College of Medicine) ;
  • Hong, Sung-Tae (Department of Parasitology and Tropical Medicine, Seoul National University College of Medicine) ;
  • Chung, Yong-Je (Department of Biochemistry, School of Natural Sciences, Chungbuk National University)
  • Published : 2007.09.30
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Abstract

The WD40-repeat proteins serve as a platform coordinating partner proteins and are involved in a range of regulatory cellular functions. A WD40-repeat protein (CsWD1) of Clonorchis sinensis previously cloned is expressed stage-specifically in the tegumental syncytium of C. sinensis metacercariae. In the present study, interact-ing proteins with the CsWD1 protein was purified by immunoprecipitation and 2 dimension gel electrophoresis from the C. sinensis metacercaria soluble extract, and tryptic peptides were analyzed by LC/ESI-MS. Putative partner proteins were annotated to be actin-2, glyceraldehyde-3-phosphate dehydrogenase, and hypothetical and unmanned proteins. The CsWD1 protein was predicted to contain 3 conserved actin-interacting residues on its functional surface. With these results, the CsWD1 protein is suggested to be an actin-interacting protein of C. sinensis.

Keywords

References

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