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Selection of Resistant Rootstock and Development of Overwintering Methods for Control of Crown Gall Disease on Grapevine

거봉의 뿌리혹병 방제를 위한 저항성 대목 선발 및 월동법

  • Kang, Sung-Su (Agricultural Development and Technology Center) ;
  • Park, Sang-Heon (Agricultural Development and Technology Center) ;
  • Park, Mun-Kyun (Agricultural Development and Technology Center) ;
  • Park, Tae-Jin (Agricultural Development and Technology Center) ;
  • Kang, Hee-Wan (Graduate School of Biotechnology and Information Technology, Hankyong National University) ;
  • Choi, Jae-Eul (College of Agriculture, Chungnam National University)
  • 강성수 (천안시농업기술센터) ;
  • 박상헌 (천안시농업기술센터) ;
  • 박문균 (천안시농업기술센터) ;
  • 박태진 (천안시농업기술센터) ;
  • 강희완 (한경대학교 생물환경정보통신전문대학원) ;
  • 최재을 (충남대학교 농업생명과학대학)
  • Published : 2007.08.01

Abstract

Grapevines will experience various types of winter damage. Some winter damages are caused by mechanical injury, freezing temperatures or poor vine vigor. This research was conducted to find out the appropriate control methods through selection of resistant rootstocks and improvement of overwintering methods for the control of crown gall disease on 'Kyoho' grape. The crown gall symptoms were not found when three stock plants of grapevine SO4, 5BB and 3306 were inoculated with $10^4cfu/ml$ of Agrobacterium vitis strains (YK2823, YK3312, LMG259, HKA234). But when they were inoculated with higher concentration $(10^6 cfu/ml)$ of A. vitis, irrespective of stocks plants, crown galls were formed all of them and the gall size was much smaller than that of kyoho. Three stock plants were selected as resistant based on above mentioned. Covering trunks and branches with rice straw and insulating coverlet was the most effective method for prevention of crown gall disease. This treatment minimized the ambient temperature changes on grapevine trees during winter season to $9.6^{\circ}C$ and the normal plant growth was due to the absence of freezing injury.

포도나무의 추위피해는 여러 형태로 나타난다. 월동피해 중에는 기계적 피해, 동해 또는 수세의 약화 원인이 된다. 본 연구는 '거봉'의 뿌리혹병의 피해를 억제하기 위하여 저항성 대목의 선발과 월동법 개선을 통한 방제법을 개발하기 위하여 실시하였다. SO4, 5BB, 3306대목에 $10^4cfu/ml$의 뿌리혹병균(YK2823, YK3312, LMG259, HKA234)을 접종했을 때는 혹이 발병하지 않았으나 $10^6cfu/ml$의 높은 농도에서는 모든 대목에서 혹이 형성된다. 그러나 혹의 크기는 거봉에서보다 매우 작았다. 인공접종에 의하여 3품종의 뿌리혹병 저항성 대목을 선발하였다. 볏짚과 보온덮개로 줄기와 가지에 피복하는 월동법은 뿌리 혹병을 예방하는데 가장 효과적이었다. 이러한 처리방법은 포도나무가 월동하는 동안 수체의 온도 차이가 $9.6^{\circ}C$로 온도변화가 최소화되었고 동해발생이 없어 정상적으로 생육하였다.

Keywords

References

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