Macromolecular Research

The Polymer Society of Korea (한국고분자학회)
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pH-Sensitivity Control of PEG-Poly(${\beta}$-amino ester) Block Copolymer Micelle

  • Hwang, Su-Jong (Department of Polymer Science & Engineering, Sungkyunkwan University) ;
  • Kim, Min-Sang (Department of Polymer Science & Engineering, Sungkyunkwan University) ;
  • Han, Jong-Kwon (Department of Polymer Science & Engineering, Sungkyunkwan University) ;
  • Lee, Doo-Sung (Department of Polymer Science & Engineering, Sungkyunkwan University) ;
  • Kim, Bong-Sup (Polymer Technology Institute, Sungkyunkwan University) ;
  • Choi, Eun-Kyung (Department of Radiation Oncology, University of Ulsan) ;
  • Park, Heon-Joo (Department of Microbiology, College of Medicine, Inha University) ;
  • Kim, Jin-Seok (College of Pharmacy, Sookmyung Women's University)
  • Published : 2007.08.31
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Abstract

Poly(ethylene glycol) methyl ether (PEG)-poly(${\beta}$-amino ester) (PAE) block copolymers were synthesized using a Michael-type step polymerization, and the construction of pH-sensitive polymeric micelles (PM) investigated. The ${\beta}$-amino ester block of the block copolymers functioned as a pH-sensitive moiety as well as a hydrophobic block in relation to the ionization of PAE, while PEG acted as a hydrophilic block, regardless of ionization. The synthesized polymers were characterized using $^1H-NMR$, with their molecular weights measured using gel permeation chromatography. The $pK_b$ values of the pH-sensitive polymers were measured using a titration method. The pH-sensitivity and critical micelle concentration (CMC) of the block copolymers in PBS solution were estimated using fluorescence spectroscopy. The pH dependent micellization behaviors with various bisacrylate esters varied within a narrow pH range. The critical micelle concentration at pH 7.4 decreased from 0.032 to 0.004 mg/mL on increasing the number of methyl group in the bisacrylate from 4 to 10. Also, the particle size of the block copolymer micelles was determined using dynamic light scattering (DLS). The DLS results revealed the micelles had an average size below 100 nm. These pH-sensitive polymeric micelles may be good carriers for the delivery of an anticancer drug.

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