International Journal of Oral Biology

The Korean Academy of Oral Biology (대한구강생물학회)
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Mutantional analysis of tumor suppressor gene p53 in human oral squamous carcinoma cell line YD-9

  • Min, Ji-Hak (Department of Oral Anatomy, School of Dentistry, Pusan National University) ;
  • Kim, Do-Kyun (Department of Oral Anatomy, School of Dentistry, Pusan National University) ;
  • Lee, Moo-Hyung (Department of Oral Anatomy, School of Dentistry, Pusan National University) ;
  • Bae, Moon-Kyoung (Department of Oral Physiology, School of Dentistry and Research Institute for Oral Biotechnology, Pusan National University) ;
  • Um, Kyung-Il (Department of Biology, College of Natural Science, Dong-A University) ;
  • Kwak, Hyun-Ho (Department of Oral Anatomy, School of Dentistry, Pusan National University) ;
  • Park, Bong-Soo (Department of Oral Anatomy, School of Dentistry, Pusan National University) ;
  • Kim, Gyoo-Cheon (Department of Oral Anatomy, School of Dentistry, Pusan National University)
  • Published : 2007.06.30
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Abstract

Oral squamous carcinoma (OSC) is the most common malignant neoplasm of the oral mucosa. Although the etiology of OSC is not fully understood, accumulated evidences indicate that the activation of proto-oncogenes and the inactivation of tumor suppressor genes underlie the disease development. An OSC cell line, YD-9 was newly established and characterized. However, the mutational analysis of p53 gene was not performed. Thus, in this study, the presence of mutation in the p53 gene was examined by amplification of exon-4 to -8 and subsequent DNA sequencing. Two point mutations were found in exon-4 and -6: A to G, resulting in amino acid change Tyr to Cys in exon-4, and C to G, resulting in amino acid change Gly to Arg in exon-6, respectively. Any mutation was not found in the exon-5, -7 and -8. The presented results would contribute to basic research to understand the biological mechanism of OSC using YD-9 cells.

Keywords

References

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