Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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Plant Regeneration from Callus and Adventitious Root Segments of Pulsatilla Koreana Nakai

  • Jung, Su-Jin (Division of Forest Resources, College of Forest and Environmental Sciences, Kangwon National University) ;
  • Jeong, Jae-Hun (Department of the Development of Medicinal Resource and Horticulture, Namdo Provincial College) ;
  • Yoon, Eui-Soo (Department of Biology, Kongju National University) ;
  • Choi, Yong-Eui (Division of Forest Resources, College of Forest and Environmental Sciences, Kangwon National University)
  • Published : 2007.06.30
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Abstract

Plant regeneration of Pulsatilla koreana was achieved via adventitious shoot formation indirectly from callus and directly from adventitious root segments. For the callus induction from leaf or petiole explants, combination of 2,4- dichlorophenoxyaceticacid (2,4-D) with $2.22\;{\mu}M$ 6-benzyladenine (BA) was effective. Adventitious shoot induction from callus was enhanced by the combined treatment with $0.1\;{\mu}M$ polyvinylpyrrolidone (PVP) compared to cytokinin treatment alone. Adventitious roots were induced from the petiole segments on 1/2 MS medium with $4.93\;{\mu}M$ IBA. High frequency direct adventitious shoot formation from the segments of adventitious roots was achieved on medium with $4.92\;{\mu}M$ 2-isopentenyladenine (2-ip). Elongated shoots were rooted on half-strength MS medium containing $5.71\;{\mu}M$ indole acetic acid (IAA). Regenerated plantlets with well-developed shoots and roots were successfully transferred to soil. This in vitro propagation protocol might be useful for mass propagation as well as conservation of this plant.

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References

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