Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Production of Monoclonal Antibody Against Listeria monocytogenes and Its Application to Immunochromatography Strip Test

  • Shim, Won-Bo (Division of Applied Life Science (Brain Korea 21 Program), Graduate School of Gyeongsang National University) ;
  • Choi, Jin-Gil (Division of Applied Life Science (Brain Korea 21 Program), Graduate School of Gyeongsang National University) ;
  • Kim, Ji-Young (Division of Applied Life Science (Brain Korea 21 Program), Graduate School of Gyeongsang National University) ;
  • Yang, Zheng-You (Division of Applied Life Science (Brain Korea 21 Program), Graduate School of Gyeongsang National University) ;
  • Lee, Kyu-Ho (Department of Environmental Engineering and Biotechnology, Hankuk University of Foreign Studies) ;
  • Kim, Min-Gon (Laboratory of Integrative Biotechnology, Korea Research Institute of Bioscience and Biotechnology) ;
  • Ha, Sang-Do (Department of Food Science and Technology, Chung-Ang University) ;
  • Kim, Keun-Sung (Department of Food Science and Technology, Chung-Ang University) ;
  • Kim, Kwang-Yup (Department of Food Science and Technology, Chungbuk University) ;
  • Kim, Cheol-Ho (Department of Biological Sciences, Sungkyunkwan University) ;
  • Ha, Kwang-Soo (Division of Applied Life Science (Brain Korea 21 Program), Graduate School of Gyeongsang National University) ;
  • Eremin, Sergei A. (Division of Chemical Enzymology, Faculty of Chemistry, M. V. Lomonosov Moscow State University) ;
  • Chung, Duck-Hwa (Division of Applied Life Science (Brain Korea 21 Program), Graduate School of Gyeongsang National University)
  • Published : 2007.07.31
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Abstract

An immunochromatography (ICG) strip test based on a monoclonal antibody for the rapid detection of L. monocytogenes in meat and processed-meat samples was developed in this study. A monoclonal antibody (MAb) specific to L. monocytogenes was produced from cloned hybridoma cells (FKLM-3B12-37) and used to develop an ICG strip test. The antibody showed a stronger binding to L. monocytogenes than other Listeria species, and a weak cross-reaction to S. aureus based on an ELISA. The detection limit of the ICG strip test was $10^5\;cell/ml$. In total, 116 meat and processed-meat samples were collected and analyzed using both the ICG strip test and a PCR. The ICG strip test and PCR indicated L. monocytogenes contamination in 34 and 27 meat samples, respectively. The 7 meat samples not identified as L. monocytogenes positive by the PCR were also tested using an API kit and found to be contaminated by Listeria species. In conclusion, the ICG strip test results agreed well with those obtained using the PCR and API kit. Thus, the developed ICG has potential use as a primary screening tool for L. monocytogenes in various foods and agricultural products, generating results within 20 min without complicated steps.

Keywords

References

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