Korean Journal of Microbiology (미생물학회지)

The Microbiological Society of Korea (한국미생물학회)
권호 표지

Effect of Citrus Fermented by Lactococcus lactis W-44 Isolated from Kimchi on Growth of Cultured Flounder, Paralichthys olivaceus

김치에서 분리한 Lactococcus lactis W-44에 의한 감귤발효물의 양식 넙치 성장에 미치는 영향

  • Kim, Min-Soo (Korea Research Institute of Bioscience and Biotechnology) ;
  • Moon, Sang-Wook (Fermentech Co.) ;
  • Lee, Young-Don (Marine and Environment Research Institute, Cheju National University) ;
  • Kim, Se-Jae (Deparaent of Biological Science, Cheju National University) ;
  • Kim, Yeong-Jin (Department of Microbiology and Immunology, College of Medicine, Pusan National University) ;
  • Lee, Jun-Won (Medical Research Center For Gene Regulation, Medical School, Chonnam National University) ;
  • Lee, Jeong-Hee (Il Hae Co.) ;
  • Lee, Jung-Sook (Korea Research Institute of Bioscience and Biotechnology) ;
  • Kim, Bo-Yeon (Korea Research Institute of Bioscience and Biotechnology) ;
  • Ahn, Jong-Seog (Korea Research Institute of Bioscience and Biotechnology) ;
  • Ahn, Soon-Cheol (Department of Microbiology and Immunology, College of Medicine, Pusan National University)
  • 김민수 (한국생명공학연구원) ;
  • 문상욱 ((주)Fermentech) ;
  • 이영돈 (제주대학교 해양과환경연구소) ;
  • 김세재 (제주대학교 생명과학과) ;
  • 김영진 (부산대학교 의과대학 미생물학 및 면역학 교실) ;
  • 이준원 (전남대학교 의과대학 유전자제어 의과학연구센터) ;
  • 이정희 ((주)일해) ;
  • 이정숙 (한국생명공학연구원) ;
  • 김보연 (한국생명공학연구원) ;
  • 안종석 (한국생명공학연구원) ;
  • 안순철 (부산대학교 의과대학 미생물학 및 면역학 교실)
  • Published : 2007.06.30
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Abstract

We evaluated the use of citrus fruit fermented by lactic acid bacteria, as a feed supplement for flounder (Paralichthys olivaceus) cultivation. For the fermentation, a lactic acid bacterial strain W-44 showing antibacterial activity was isolated from kimchi. From the phylogenetic analysis based on, 16S rDNA sequence, the strain W-44 was identified as Lactococcus lactis. After the fermentation of citrus fruit with L. lactis W-44, the contents of naringenin and hesperetin, bioactive flavonoid aglycones, were increased about ten-fold and six-fold, respectively. The effects of fermented citrus fruit-based feed additives (CFBFA) were tested on the growth of flounder, Paralichthys olivaceus. There were significant differences in average total length and body weight between the experimental and control group. The growth rate of the experimental group fed with the 0.2% CFBFA-supplemented diet was increased 4.5% and 20.9% more than the control group in total length and body weight, respectively. These results suggest that the fermented citrus fruit could be used as a functional feed additive for flounder cultivation.

젖산균을 접종하여 감귤 분쇄액을 발효시켜 얻은 감귤발효물의 어류양식 사료 첨가제로의 가능성을 넙치(Paralichthys olivaceus) 양식에서 검토하였다. 김치에서 항균활성이 우수한 젖산균 W-44를 분리한 후 16S rDNA 염기서열 분식을 통하여 Lactococcus lactis W-44로 동정하였다. L. lactis W-44를 이용하여 감귤 분쇄액 발효를 수행한 결과, 생리활성이 우수한 무배당체 flavonoids인 naringenin, hesperitin의 함량이 각각 약 10과 6배 증가함을 확인하였다. L. lactis W-44를 이용하여 발효된 감귤발효액을 양식넙치의 사료첨가제로 투여한 결과, 감귤발효액을 투여하지 않은 대조구의 평균 전장 및 체중 증가율과 현격한 차이가 있었다. 또한 0.2% (v/v)의 감귤발효액을 투여한 실험군에서 넙치의 성장이 가장 우수한 것으로 나타났다. 이러한 결과로부터 젖산균의 감귤발효산물을 넙치 양식에서 기능성 사료첨가제로 사용할 수 있는 가능성을 확인하였다.

Keywords

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