금은화 수용성 추출물의 LPS 유도 염증매개물 억제 효과

Inhibitory Effect of Aqueous Extract from Lonicera japonica Flower on LPS-induced Inflammatory Mediators in RAW 264.7 Macrophages.

  • 윤용갑 (원광대학교 한의과대학 방제학교실) ;
  • 김규민 (원광대학교 한의과대학 방제학교실) ;
  • 이성준 (원광대학교 한의과대학 방제학교실) ;
  • 유승훈 (원광대학교 한의과대학 방제학교실) ;
  • 장선일 (전주대학교 대체의학대학 대체건강관리학부 건강관리전공)
  • Yun, Young-Gab (Department of Oriental Medical Prescription, Wonkwang University) ;
  • Kim, Gyu-Min (Department of Oriental Medical Prescription, Wonkwang University) ;
  • Lee, Sung-Jun (Department of Oriental Medical Prescription, Wonkwang University) ;
  • Ryu, Seong-Hun (Department of Oriental Medical Prescription, Wonkwang University) ;
  • Jang, Seon-Il (School of Alternative Medicine & Health Science, College of Alternative Medicine, Jeonju University)
  • 발행 : 2007.09.28

초록

Objective : Lonicera japonica (Caprifoliaceae) has long been used for treatment of infectious diseases in oriental countries. The aim of this study was to investigative the effect by which the aqueous extract from flower of L. japonica (LJFAE) inhibited the lipopolysaccharide (LPS)-induced inflammatory mediators in murine macrophages, RAW 264.7 cells Methods : The dried flowers of L. japonica were extracted with distilled water at $100^{\circ}C$ for 7 h. The extract was filtered through 0.45 ${\mu}m$ filter, freeze-dried. The dried extract was dissolved in Hank's balanced salt solution (HBSS) and filtered through 0.22 ${\mu}m$ filter before use. Accumulated nitrite, an oxidative product of nitric oxide (NO), was measured in the culture medium by the Griess reaction. The levels of prostaglandin E2 (PGE2), tumor necrosis factor-$\alpha$ (TNF-$\alpha$), interleukin-1$\beta$ (IL-1$\beta$), and IL-6 production, inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) expression were measured by enzyme-linked immunosorbent assay and Western blot analysis. Results: LJFAE (10-400 ${\mu}g$/ml) per se had no cytotoxic effect in unstimulated macrophages, but LJFAE concentration-dependently reduced NO, PGE2, TNF-, IL-l, and IL-6 production and COX-2 activity caused by stimulation of LPS. The levels of iNOS and COX-2 protein expressions were markedly suppressed by the treatment with LJFAE in a concentration dependent manner. Conclusions : These results suggest that LJFAE suppress the NO and PGE2production in macrophages by inhibiting iNOS and COX-2 expression and these properties may contribute to the anti-inflammatory activity of Lonicera japonica.

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