Tuberculosis and Respiratory Diseases

  • 제62권3호
  • /
  • Pages.197-202
  • /
  • 2007
  • /
  • 1738-3536(pISSN)
  • /
  • 2005-6184(eISSN)
대한결핵및호흡기학회 (The Korean Academy of Tuberculosis and Respiratory Diseases)
권호 표지

흡연성분 중 Nicotine, Cotinine, Benzopyrene이 인체 기관지 상피세포에서 항산화제의 발현에 미치는 영향

Effects of Nicotine, Cotinine and Benzopyrene as Smoke Components on the Expression of Antioxidants in Human Bronchial Epithelial Cells

  • 김용석 (한양대학교 의과대학 생화학분자생물학교실) ;
  • 이재형 (한양대학교 의과대학 내과학교실) ;
  • 김상헌 (한양대학교 의과대학 내과학교실) ;
  • 김태형 (한양대학교 의과대학 내과학교실) ;
  • 손장원 (한양대학교 의과대학 내과학교실) ;
  • 윤호주 (한양대학교 의과대학 내과학교실) ;
  • 박성수 (한양대학교 의과대학 내과학교실) ;
  • 신동호 (한양대학교 의과대학 내과학교실)
  • Kim, Yong Seok (Department of Biochemistry and Molecular Biology, College of Medicine, Hanyang University) ;
  • Lee, Jae Hyung (Department of Internal Medicine, College of Medicine, Hanyang University) ;
  • Kim, Sang Heon (Department of Internal Medicine, College of Medicine, Hanyang University) ;
  • Kim, Tae Hyung (Department of Internal Medicine, College of Medicine, Hanyang University) ;
  • Sohn, Jang Won (Department of Internal Medicine, College of Medicine, Hanyang University) ;
  • Yoon, Ho Joo (Department of Internal Medicine, College of Medicine, Hanyang University) ;
  • Park, Sung Soo (Department of Internal Medicine, College of Medicine, Hanyang University) ;
  • Shin, Dong Ho (Department of Internal Medicine, College of Medicine, Hanyang University)
  • 투고 : 2006.12.11
  • 심사 : 2007.01.26
  • 발행 : 2007.03.30
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초록

흡연물질로서 benzopyrene, nicotine 및 cotinine등은 기관지상피세포인 Beas2B에서 CuZnSOD, thioredoxin, glutathione reductase 등의 발현량에 영향을 주며 특히 thioredoxin 및 glutathione reductase의 발현량을 노출 후 30분에서 4시간 경과 시간대에 증가시켰다가 24시간 이후에는 억제하는 특징을 나타내었다. 상기한 흡연물질에 의한 항산화효소의 조절기전에는 전사인자인 NNF-${\kappa}B$가 관여하는 것으로 추정되었다.

Background: Cigarette smoking is an important risk factor for chronic bronchitis and COPD. Airway epithelial cells exposed to cigarette smoke components such as nicotine, cotinine and benzopyrene can generate reactive oxygen species (ROS) and be subject to oxidative stress. This oxidative stress can induce the inflammatory response in the lung by the oxidant itself or by the release of proinflammatory cytokines. It has been reported that nicotine stimulates ROS, which are associated with NF-${\kappa}B$. Methods: Beas2B cells were treated with nicotine, cotinine and benzopyrene. RT PCR was used to measure the expression of several antioxidant factors using the total RNA from the Beas2B cells. The level of superoxide dismutase(CuZnSOD), thioredoxin, glutathione reductase expression was examined. Results: 0.5 to 4 hours after the benzopyrene, nicotine and cotinine theatments, the level of thioredoxin and glutathione reductase expression decreased. Longer exposure to these compounds for 24 to 72 hours inhibited the expression of most of these antioxidant factors. Conclusion: During exposure to smoke compounds, thioredoxin and glutathione reductase are the key antioxidant factors induced sensitively between 0.5 and 4 hours but the levels these antioxidants decrease between 24 hour and 72hours.

키워드

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