한국산림과학회지 (Journal of Korean Society of Forest Science)

  • 제96권2호
  • /
  • Pages.131-137
  • /
  • 2007
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  • 2586-6613(pISSN)
  • /
  • 2586-6621(eISSN)
한국산림과학회 (Korean Society of Forest Science)
권호 표지

Variation in trn-L/trn-V and trn-F/trn-T spacer regions of cpDNA in Abies koreana Wilson and A. nephrolepis Traut./Maxim

  • Kormutak, A. (Institute of Plant Genetics and Biotechnology Slovak Academy of Sciences) ;
  • Hong, Y.-P. (Korea Forest Research Institute, Department of Forest Genetic Resources) ;
  • Kwon, H.-Y. (Korea Forest Research Institute, Department of Forest Genetic Resources) ;
  • Kim, C.-S. (Warm-temperature Forest Research Center, Korea Forestry Research Institute)
  • 투고 : 2005.09.30
  • 심사 : 2006.07.18
  • 발행 : 2007.06.30
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초록

The first evidence has been provided about the variation within trnL-trnV and trnF-trnT spacer regions of cpDNAs in Korean fir and Manchurian fir, revealed by PCR-RFLP analysis. Four cpDNA haplotypes have accordingly been recognized by being analyzed using the trnL-trnV/Tru11 primer-enzyme combination and 3 haplotypes using the trnF-trnT/TagI combination, which exhibited inter and intraspecific variation. A total of 6 cpDNA haplotypes were recognized by pooling the PCR-RFLP variants observed in both combinations. Haplotypes 2 and 3 were common for both species investigated, whereas haplotypes 1, 4, and 5 were detected only in Korean fir and haplotype 6 was detected only in Manchurian fir. Although haplotypes 2 and 3 were common in both species, haplotype 2 was major haplotype for Korean fir and haplotype 3 was one of the 2 major haplotypes for Manchurian fir. Restricted occurrence of haplotype 4 in Mt. Halla and haplotype 5 in Mt. Jiri of the Korean fir may represent the existence of geographic isolation by the sea between them. Diagnostic potential of individual haplotypes in discriminating between the two species as well as between their populations is discussed.

키워드

참고문헌

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