Journal of the Korean Society of Visualization (한국가시화정보학회지)

The Korean Society of Visualization (한국가시화정보학회)
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Understanding Diffusion in Cells and Living Tissues

세포 및 생체조직에서 확산에 관한 이해

  • 김중경 (국민대학교 기계자동차공학부)
  • Published : 2007.06.30
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Abstract

Macromolecule diffusion in cells and tissues is important for cell signaling, metabolism and locomotion. Biophysical methods, including non-invasive or minimally invasive in-vivo photobleaching techniques and single quantum-dot tracking, have been used to measure the rates of macromolecule diffusion in living cells and tissues, including central nervous system and tumors. Mathematical modeling and statistical analysis of experimental data revealed various modes of diffusion, which are strongly coupled with spatiotemporal changes in nanoscale structures and material properties.

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References

  1. Verkhovsky, A. B., Svitkina, T. M. and Borisy, G. G., 1999, "Self-polarization and Directional Motility of Cytoplasm," Curr. Biol., Vol.9(1), pp.11-20. https://doi.org/10.1016/S0960-9822(99)80042-6
  2. Jiang, X., Bruzewicz, D. A., Wong, A. P., Piel, M. and Whitesides, G. M., 2005, "Directing Cell Migration with Asymmetric Micropatterns," Proc. Natl. Acad. Sci. USA, Vol.102(4), pp.975-978. https://doi.org/10.1073/pnas.0408954102
  3. Nelson, C. M., Vanduijn, M. M., Inman, J. L., Fletcher, D. A. and Bissell, M. J., 2006, "Tissue Geometry Determines Sites of Mammary Branching Morphogenesis in Organotypic Cultures," Science, Vol.314(5797), pp.298-300. https://doi.org/10.1126/science.1131000
  4. Meyers, J., Craig, J. and Odde, D. J.. 2006, "Potential for Control of Signaling Pathways via Cell Size and Shape," Curr. Biol., Vol.6(17), pp.1685-1693.
  5. Papadopoulos, M. C.*, Kim, J. K.* and Verkman, A. S., 2005, "Extracellular Space Diffusion in the CNS: Anisotropic Diffusion Measured by Elliptical Surface Photobleaching," Biophys. J., Vol.89(5), pp. 3660-3668. (*Authors contributed equally) https://doi.org/10.1529/biophysj.105.068114
  6. Truskey, G. A., Yuan F. and Katz, D. F., 2004, Transport Phenomena in Biological Systems, Pearson Prentice Hall, New Jersey, pp.690.
  7. Thiagarajah, J. R.*, Kim, J. K.*, Magzoub, M. and Verkman, A. S., 2006, "Slowed Diffusion in Tumors Revealed by Microfiberoptic Epifluorescence Photobleaching," Nat. Methods, Vol.3(4), pp.275-280. (*Authors contributed equally) https://doi.org/10.1038/nmeth863
  8. Haggie, P. M., Kim, J. K., Lukacs, G. L. and Verkman, A. S., 2006, "Tracking of Quantum Dot- Labeled CFTR Shows Near Immobilization by Cterminal PDZ Interactions," Mol. Biol. Cell, Vol. 17(12), pp.4937- 4945. https://doi.org/10.1091/mbc.E06-08-0670

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