Journal of Embryo Transfer (한국수정란이식학회지)

The Korean Society of Embryo Transfer (한국수정란이식학회)
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Effect of BPA and Nicotine on In Vitro Maturation of Porcine Oocytes

BPA 및 Nicotine 첨가가 돼지 난자의 체외 성숙에 미치는 영향

  • Lee, H.J. (College of Veterinary Medicine, Chungnam National University) ;
  • Lee, H.R. (College of Veterinary Medicine, Chungnam National University) ;
  • Han, W.R. (College of Veterinary Medicine, Chungnam National University) ;
  • Rhee, M.H. (College of Veterinary Medicine, Kyungpook National University) ;
  • Kim, S.K. (College of Veterinary Medicine, Chungnam National University)
  • Published : 2007.06.30
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Abstract

본 연구는 BPA 및 nicotine 첨가 농도와 배양 시간이 돼지 난자의 체외성숙에 미치는 영향을 조사하였다. $0.02{\sim}10.0mM$ BPA와 $0.5{\sim}10.0mM$ nicotine이 첨가된 TCM-199배양액에서 $40{\sim}52$시간 난자를 배양했을 때 체외성 숙율을 조사하였다. BPA농도가 높을수록 체외성숙율이 유의적으로 낮게 나타났다. $0.05{\sim}10.0nM$ BPA를 첨가한 TCM-199 배양액에서 난자를 44시간 배양했을 때 체외성숙율은 각각 $40.0{\pm}4.1%,\;24.0{\pm}4.7%,\;10.0{\pm}5.3%,\;6.0{\pm}3.2%,\;0.0{\pm}0.0%$로서 첨가 농도가 증가할수록 낮은 체외성숙율을 나타냈다. 난자를 $0.5{\pm}10.0mM$ nicotine를 첨가한 TCM-199 배양액에서 44시간 배양했을 때 체외성숙율은 각각 $44.0{\pm}4.5%,\;24.0{\pm}4.2%,\;18.0{\pm}4.9%,\;8.0{\pm}2.2%,\;0.0{\pm}0.0%$로서 대조군$(52.0{\pm}4.5%)$에 비해 낮은 체외성숙율을 나타냈다. 난자를 0.5 nM BPA와 2.5 mM nicotine을 첨가한 TCM-199에서 $40{\sim}52$시간 배양했을 때 체외성숙율은 $8.3{\pm}2.1%{\sim}26.0{\pm}3.9%$ 및 $11.2{\pm}2.2%{\sim}28.6{\pm}3.9%$로서, 44시간 배양이 다른 배양시간보다 가장 높은 체외성숙율을 나타냈다.

Keywords

References

  1. Arabi M and Moshtaghi H. 2005. Influence of cigarette smoking on spermatozoa via seminal plasma. Andrologia, 37: 119-124 https://doi.org/10.1111/j.1439-0272.2005.00664.x
  2. Augood C, Duckitt K and Templeton A. 1998. Smoking and female infertility: review and meta-analysis. Hum. Reprod., 13:1532-1539 https://doi.org/10.1093/humrep/13.6.1532
  3. Baek HJ, Hwang IJ, Kim KS, Lee YD, Kim HB and Yoo MS. 2007. Effects of BPA and DES on longchin goby (Chasmichthys dolichognathus) in vitro during the oocyte maturation. Marine Environmental Research, 64:79-86 https://doi.org/10.1016/j.marenvres.2007.01.006
  4. Baldwin KV and Racowsky C. 1998. Nicotine and cotinine effects on development of two-cell mouse embryos in vitro. Reprod. Toxicol., 1:173-178 https://doi.org/10.1016/S0890-6238(87)80029-1
  5. Bishun NP, Lloyd N, Raven RW and Williams DC. 1972. The in vitro and in vivo cytogenetic effects of nicotine. Acta. Biol. Acad. Hung., 23:175-180 https://doi.org/10.1007/BF01889913
  6. Joesbury KA, Edirisinghe WR, Phillips MR and Yovich JL. 1998. Evidence that male smoking affects the likelihood of a pregnancy following IVF treatment: application of the modified cumulative embryo score. Hum. Reprod., 13:1506-1513 https://doi.org/10.1093/humrep/13.6.1506
  7. Klonoff-Cohen H, Natarajan L, Marrs R and Yee B. 2001. Effects of female and male smoking on success rates of IVF and gamete intra-fallopian transfer. Reproduction, 16: 1382-1390
  8. Krishnan P, Starhis SF, Permuth L, Tokes and Feldman D. 1993 Bisphenol-A: an estrogenic substance is released from polycarbonate flasks during autoclaving. Endocrinology, 132:2279 - 2286 https://doi.org/10.1210/en.132.6.2279
  9. Liu Y, Li GP, Rickords LF, White KL, Sessions BR, Aston KI and Bunch TD. 2006. Effect of nicotine on in vitro maturation of bovine oocytes. Anim. Reprod. Sci., 26:[Epub ahead of print]
  10. Mailhes J, Young D, Caldito G and London SN. 2000. Sensitivity of mouse oocytes to nicotine-induced perturbations during oocyte meiotic maturation and aneuploidy in vivo and in vitro. Mol. Hum. Reprod., 6:232-237 https://doi.org/10.1093/molehr/6.3.232
  11. Mailhes J, Young D, Caldito G and London SN. 2005. Sensitivity of mouse oocytes to nicotine-induced perturbations during oocyte meiotic maturation and aneuploidy in vivo and in vitro. Mol. Hum. Reprod., 6:232-237 https://doi.org/10.1093/molehr/6.3.232
  12. Miceli F, Minici F, Tropea A, Catino S, Orlando M, Lamanna G, Sagnella F, Tiberi F, Bompiani A, Mancuso S, Lanzone A and Apa R. 2005. Effects of nicotine on human luteal cells in vitro: a possible role on reproductive outcome for smoking women. Biol. Reprod., 72:628-632 https://doi.org/10.1095/biolreprod.104.032318
  13. Racowsky C, Hendricks RC and Baldwin KV. 1989. Direct effects of nicotine on the meiotic maturation of hamster oocytes. Reprod. Toxicol., 3:13-21 https://doi.org/10.1016/0890-6238(89)90033-6
  14. Shiloh H, Baratz SL, Koifman M, Ishai D, Bidder D, WeinerMeganzi Z and Dimfeld M. 2004. The impact of cigarette smoking on zona pellucida thickness of oocytes and embryos prior to transfer into the uterine cavity. Hum. Reprod., 19:157-159 https://doi.org/10.1093/humrep/deh029
  15. Tuormaa TE. 1995. The adverse effects of tobacco smoking on reproduction and health: A review from the literature. Nutr. Health, 10: 105-120 https://doi.org/10.1177/026010609501000202
  16. Zhao Z and Reece EA. 2005. Nicotine-induced embryonic malformations mediated by apoptosis from increasing intracellular calcium and oxidative stress. Birth Defects Res., 74: 383-391 https://doi.org/10.1002/bdrb.20052