Quantitative Analysis of chiisanoside in Acanthopanax Species by HPLC

  • Lee, Jeong-Min (Department of Applied Plant Science, College of Industrial Science, Chung-Ang University) ;
  • Kim, Hye-Min (Department of Applied Plant Science, College of Industrial Science, Chung-Ang University) ;
  • Lee, Sang-Hyun (Department of Applied Plant Science, College of Industrial Science, Chung-Ang University)
  • 발행 : 2007.06.30

초록

High performance liquid chromatography (HPLC) was used for the analysis of chiisanoside in Acanthopanax species. A reverse-phase system using a gradient of H$_2$O and acetonitrile as the mobile phase was developed and a wavelength of detection was at 210 nm. The analysis was successfully carried out for 30 min. Chiisanoside was measured in the fruit, stem and root of A. sessiliflorus, A. koreanus, A. divaricatus and A. senticosus.

키워드

참고문헌

  1. Bae, E.A., Yook, C.S., Oh, O.J., Chang, S.Y., Nohara, T., and Kim, D.H., Metabolism of chiisanoside from Acanthopanax divaricatus var. albeofructus by human intestinal bacteria and its relation to some biological activities. Biol. Pharm. Bull. 24, 582-585 (2001) https://doi.org/10.1248/bpb.24.582
  2. Davydov, M. and Krikorian, A.D., Eleutherococcus senticosus (Rupr. & Maxim.) Harms (Araliaceae) as an adoptogen: a closer look. J. Ethnopharmacol. 72, 345-393 (2000) https://doi.org/10.1016/S0378-8741(00)00181-1
  3. Hahn, D.R., Kasai, R., Kim, J.H., Taniyasu, S., and Tanaka, O., A new glycosyl ester of a 3,4-seco-triterpene from Korean medicinal plant, Acanthopanax chiisanensis. Chem. Pharm. Bull. 32, 1244-1247 (1984) https://doi.org/10.1248/cpb.32.1244
  4. Jiang, W., Li, W., Han, L., Liu, L., Zhang, Q., Zhang, S., Nikaido, T., and Koike, K., Biologically active triterpenoid saponins from Acanthopanax senticosus. J. Nat. Prod. 69, 1577-1581 (2006) https://doi.org/10.1021/np060195+
  5. Jin, J.L., Lee, S., Lee, Y.Y., Kim, J.M., Heo, J.E., and Yun-Choi, H.S., Platelet anti-aggregating triterpenoids from the leaves of Acanthopanax senticosus and the fruits of A. sessiliflorus. Planta Med., 70, 564-566 (2004) https://doi.org/10.1055/s-2004-827159
  6. Jung, H.J., Nam, J.H., Choi, J., Lee, K.T., and Park, H.J., Antiinflammatory effects of chiisanoside and chiisanogenin obtained from the leaves of Acanthopanax chiisanensis in the carrageenan- and Freund's complete adjuvant-induced rats. J. Ethnopharmacol. 97, 359- 367 (2005) https://doi.org/10.1016/j.jep.2004.11.026
  7. Kang, J.S., Linh, P.T., Cai, X.F., Lee, J.J., and Kim, Y.H., Determination of chiisanoside in Acanthopanax species by high performance liquid chromatography. Nat. Prod. Sci. 9, 45-48 (2003)
  8. Kim, C.J. and Hahn, D.R., The biological activity of a new glycoside, chiisanoside from Acanthopanax chiisanensis Nakai leaves. Yakhak Hoeji 24, 123-134 (1980)
  9. Kim, Y.O., Cho, D.H., Chung, H.J., Kim, J.H., Chang, S.Y., Yook, C.S., Yang, K.S., and Oh, O.J., Effects of lupine-triterpenoids on mitogeninduced proliferation of lymphocytes. Yakhak Hoeji 43, 208-239 (1999)
  10. Lee, S., Shin, D.S., Oh, K.B., and Shin, K.H., Antibacterial compounds from the leaves of Acanthopanax senticosus. Arch. Pharm. Res. 26, 40-42 (2003) https://doi.org/10.1007/BF03179929
  11. Perry, L.M. and Metzger, J., Medicinal plants of East and Southeast Asia. MIT press, Cambridge, Massachusetts and London, 1980, pp. 41
  12. Shin, K.H. and Lee, S., The chemistry of secondary products from Acanthopanax species and their pharmacological activities (Review). Nat. Prod. Sci. 8, 111-126 (2002)
  13. Shin, S.-J. and Hong, S.-T., Acanthopanax and Platycodi independently prevents the onset of high fat diet induced hyperglyceridemia and obesity in C57BL/6 mice. Food Sci. Biotechnol. 14, 841-846 (2005)
  14. Won, J.H., Park, S.Y., Nam, S.G., Park, H.J., Choi, J.W., and Lee, K.T., Inhibition of lipopolysaccharide-induced expression of inducible nitric oxide and cyclooxygenase-2 by chiisanoside via suppression of nuclear factor-kappaB activation in RAW 264.7 macrophage cells. Biol. Pharm. Bull. 28, 1919-1924 (2005) https://doi.org/10.1248/bpb.28.1919
  15. Yook, C.S., Rho, Y.S., Seo, S.H., Leem, J.Y., and Han, D.R., Chemical components of Acanthopanax divaricatus and anticancer effect in leaves. Yakhak Hoeji 40, 251-261 (1996)
  16. Yook, C.S., Coloured medicinal plants of Korea. Academy Publishing Co., Seoul, 1990, pp. 377
  17. Yoshizumi, K., Hirano, K., Ando, H., Hirai, Y., Ida, Y., Tsuji, T., Tanaka, T., Satouchi, K., and Terao, J., Lupane-type saponins from leaves of Acanthopanax sessiliflorus and their inhibitory activity on pancreatic lipase. J. Agric. Food Chem. 54, 335-341 (2006) https://doi.org/10.1021/jf052047f