생명과학회지 (Journal of Life Science)

한국생명과학회 (Korean Society of Life Science)
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운동이 F344계 임신쥐에서 골격근의 VAMP-2 및 GLUT-4 단백질 발현과 혈중 인슐린, 렙틴 농도에 미치는 영향

Effects of maternal exercise on expression of GLUT-4, VAMP-2 in skeletal muscle and plasma insulin and leptin levels in pregnant rats

  • 윤진환 (한남대학교 스포츠의학연구소) ;
  • 이희혁 (한남대학교 스포츠의학연구소) ;
  • 김종오 (한남대학교 스포츠의학연구소) ;
  • 오명진 (한남대학교 스포츠의학연구소) ;
  • 박성태 (한남대학교 스포츠의학연구소) ;
  • 지용석 (청주대학교 스포츠의학연구소) ;
  • 서태범 (대전대학교 한의대) ;
  • 남궁욱 (대전대학교 한의대)
  • Yoon, Jin-Hwan (Research Institute of Sports Medicine, Hannam University) ;
  • Lee, Hee-Hyuk (Research Institute of Sports Medicine, Hannam University) ;
  • Kim, Jong-Oh (Research Institute of Sports Medicine, Hannam University) ;
  • Oh, Myung-Jin (Research Institute of Sports Medicine, Hannam University) ;
  • Park, Seong-Tae (Research Institute of Sports Medicine, Hannam University) ;
  • Jee, Young-Seok (Research Institute of Sports Medicine, CheongJu University) ;
  • Seo, Tae-Beom (Department of Oriental Medicine, Daejeon Univeristy) ;
  • NamGung, Uk (Department of Oriental Medicine, Daejeon Univeristy)
  • 발행 : 2007.06.25
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초록

본 연구는 임신횐쥐에서 운동이 혈중 인슐린과 렙틴 농도, 그리고 골격근의 GLUT-4와 VAMP-2단백질 발현에 변화를 조사하기 위하여 수행되었다. 본 연구에서 비임신 횐쥐에 비해 임신 횐쥐에서 혈중 인슐린 농도가 유의하게 증가되었고, 렙틴 농도는 유의하게 감소하는 것으로 나타났다. 임신횐쥐에게 달리기 운동을 적용한 결과 임신으로 증가된 인슐린 농도를 유의하게 감소시키면서 렙틴 농도의 감소를 억제시키는 것으로 나타났다. 골격근에서 GLUT-4와 VAMP-2 단백질 발현량을 조사한 결과 비임신 횐쥐에 비해 임신 횐쥐에서 이러한 단백질 발현이 유의하게 감소되었지만, 임신 중 달리기운동을 경험한 횐쥐에서 GLUT-4와 VAMP-2 발현 모두 유의하게 증가되는 것으로 나타났다. 이와 잘이 임신으로 인한 혈중 인슐린 및 렙틴의 변화가 골격근에서 당을 근조직으로 흡수하는 신호 전달 경로와 수송체의 발현 손상과 연관되어있음을 보여주는 것이다. 하지만 임신 횐쥐에서 달리기 운동은 인슐린과 렙틴 농도의 변화를 완화시킴으로써 골격근의GLUT-4와 VAMP-2발현을 유의하게 증가시키는 것으로 나타났다.

The main objective of this study was to investigate the effects of exercise on serum insulin and leptin levels and GLUT-4 and VAMP-2 in skeletal muscles from the pregnant rats. F344 rats were randomly divided into four groups (n = 15 in each group): control group (CG), pregnant group (PG), pregnant running group (PR), and pregnant swimming group (PS). From the 15th day of pregnancy, animals in the running group were forced to run on treadmill for 30 min with a light intensity, while those in the swimming group were forced to swim in swimming pool for 10 min once a day for 6 consecutive days. The present result demonstrated that in pregnant rat group, serum insulin levels significantly in-creased and leptin levels significantly decreased. Skeletal GLUT-4 and VAMP-2 protein expression was significantly decreased in pregnant rats compared to non-pregnant rats. However, matenal running during gestational period alleviated pregnancy-induced changes in plasma insulin and leptin levels, and it significantly enhanced skeletal GLUT-4 and VAMP-2 protein expression. From those results, it can be suggested that running exercise during gestational period may improve glycemic control by up-regulating GLUT-4 and VAMP-2 protein expression.

키워드

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