생명과학회지 (Journal of Life Science)

한국생명과학회 (Korean Society of Life Science)
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설사환자에서 Campylobacter jejuni의 분리 및 항생제 감수성 특성

Isolation and Antimicrobial Susceptibility of Campylobacter jejuni from Diarrhea Patients

  • 박은희 (부산광역시보건환경연구원) ;
  • 김정아 (부산광역시보건환경연구원) ;
  • 최성화 (부산광역시보건환경연구원) ;
  • 빈재훈 (부산광역시보건환경연구원) ;
  • 최홍식 (부산광역시보건환경연구원) ;
  • 석동희 (인제대학교 동래백병원 진단검사의학과) ;
  • 이수철 (춘해병원 진단검사의학과) ;
  • 김영희 (한서병원 진단검사의학과)
  • Park, Eun-Hee (Department of Microbiology, Busan Metropolitan City Institute of Health & Environment) ;
  • Kim, Joung-A (Department of Microbiology, Busan Metropolitan City Institute of Health & Environment) ;
  • Choi, Seung-Hwa (Department of Microbiology, Busan Metropolitan City Institute of Health & Environment) ;
  • Bin, Jae-Hun (Department of Microbiology, Busan Metropolitan City Institute of Health & Environment) ;
  • Cheigh, Hong-Sik (Department of Microbiology, Busan Metropolitan City Institute of Health & Environment) ;
  • Suk, Dong-Hee (Department of Labolatory medicine, Inje University Dongnae Paik Hospital) ;
  • Lee, Su-Chul (Departmnet of Clinical pathology, ChoonHae Hospital) ;
  • Kim, Young-Hui (Department of Labolatory medicine, Hanseo Hospital)
  • 발행 : 2007.06.25
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초록

2005년 1월부터 2006년 10월까지 설사환자의 분변 882건에서 27주의 C. jejuni가 분리되어 3.1%의 분리율을 보였으며, 계절별로는 7월에 가장 높은 9주(11.7%)가 분리되었으며, 분리균주 모두는 catalase 양성, hippuric acid를 가수분해하였다. heat-stable antigen을 이용한 PHA법으로 확인한 혈청형은 HS2형이 18.5%로 가장 많이 분리되었으며 Hs1/44형과 HS2l형 이 각각 11.1%, HS4 complex와 HSl9형 이 각각 7.4%,그리고 HSl8형이 3.7%였으며, 40.7%인 11주는 본 실험방법으로 혈청형을 확인할 수 없었다. 항생제에 대한 내성정도는 cephalothin에는 100%의 내성률을 erythromycin과 imipenem에는 0%의 내성률을 나타내어 분리균주 모두 감수성이었다. 항생제별로는 trimethoprim-sulfamethoxazole 63.0%, tetracycline 51.9%, ciprofloxacin 37.0%, ampicillin과 nalidixic acid 33.3%, gentamycin 25.9%, clindamycin 7.4%, chlorarmphenicol 3.7%의 순으로 내성률을 보였다.

ln this study we isolated 27 isolates of Campylobacter jejuni from stool samples of 882 diarrheal patients. The seasonal distribution of patients was highest at July (11.7%). All the isolates of C. jejuni hydrolyzing sodium hippurate were serotyped on basis of heat-stable antigens, and identified with the use of passive hemagglutination assay. A total of 59.3% among 27 C. jejuni isolates were identified into 6 different serotypes, which serotype HS2, HSl/44, and HS2l were dominant. Antibiotics resistant rates of C. jejuni isolates were shown to be 100%, 63.0%, 51.9%, 37.0%, 33.3%, 25.9% and 7.4% to cephalothin, trimethoprim- sulfamethoxazole, tetracycline, ciprofloxacin, ampicillin, gentamycin and clindamycin, respectively. All isolates were sensitive to the erythromycin and imipenem.

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참고문헌

  1. Blaser, M. J., H. L. Hardesty, Bwers B. and W. I. Wang. 1980. Survival of Campylobacter jejuni subsp. jejuni in biological milieus. J. Clin. Microbiol. 11, 309-313
  2. Bokkenheuser, V. D., N. J. Richardson, J. H. Bryner, D. J. Rouk, A. B. Schute, H. J. Koongof, I. Ereiman and E. Hartman. 1979. Detection of enteric Campylobacteriosis in children. J. Clin. Microbiol. 9, 277-232
  3. Bopp, C. A., K. A. Birkenss, I. K. Wachsmuth, and T. J. Barrett. 1985. In Vitro Antimicrobial Susceptibility, Plasmid Analysis, and Serotyping of Epidemic- Associated Campylobacter jejuni. J. Clin. Microbiol. 21, 4-7
  4. Cha, I. H., Y. H. Kim, J. H. Bin, S. T. Ha, K. S. Kim, H. D. Kweon and C. N. Lee. 1994. Distribution and Susceptibility to Drugs of Food Poisoning Microorganisms Isolated from Roadside Foods in Pusan Area. J. Fd. Hyg. Safety 9, 117-121
  5. Clark, C. G., L. Price, R. Ahmed, D. L. Woodward, P. L. Melito, F.G. Rodgers, F. Jamieson, Ciebin Bruce, A. Li, and A. Ellis. 2003. Characterization of Waterborne Outbreak-associated Campylobacter jejuni, Walkerton, Ontario. Emerg. Infect. Dis. 9, 1232-1241 https://doi.org/10.3201/eid0910.020584
  6. Friedman, C. R., J. Neiemann, H. C. Wegener and R. V. Tauxe. 2000. Epidemiology of Campylobacter jejuni infections in the unites States and other industrialized nations, pp. 121-138. In I. Nachamkin and M. J. Blaser (ed.), Campylobacter, 2nd ed. American Society for Microbiology, Washing D. C.
  7. Gaudreu, C. and H. Gilbert. 1998. Antimicrobial Resistance of Clinical Strains of Campylobacter jejuni subsp. jejuni Isolated from 1985 to 1997 in Quebec, Canada. Antimicro. Agents Chemother. 42, 2106-2108
  8. Hebert, G. A., D. G. Hollis, R. E. Weaver, M. A. Lambert, M. J. Blaser and C. W. Moss. 1982. 30 Years of Campylobacters: Biochemical Characteristics and a Biotyping Proposal for Campylobacter jejuni. J. Clin. Microbiol. 15, 1065-1073
  9. Hwang, T. W., J. H. Yoon, H. S. Chun, E. S. Jang, B. K. Jun, B. G. Jung and K.S. Jung. 1997. Isolation and Plasmid DNA profile of Campylobacter jejuni. Taegu Metropolitan City of Institute of Health and Environment Report 7-22
  10. Karenlampi, R., H. Rautelin, M. Hakkinen and M. L. H a'nninen. 2003. Temporal and Geographical Distribution and Overlap of Penner Heat-Stable Serotypes and Pulsed-Field Gel Electrophoresis Genotypes of Campylobacter jejuni Isolates Collected from Humans and Chickens in Finland during a Seasonal Peak. J. Clin. Microbiol. 41, 4870-4872 https://doi.org/10.1128/JCM.41.10.4870-4872.2003
  11. Kang, ho-Jo, Y. H. Kim, B. G. Chung and C. E. Park. 1989. Epidemiological Studies on Campylobacter Enteritidis in Korea. 1. Prevalence of Campylobacter jejuni and C. coli in Human, Animals, Food and Water and Serotypes Isolated. Kor. J. Vet. Publ. Hlth. 13, 95-104
  12. Kang, ho-Jo, Y. H. Kim, S. C. Lee and C. E. Park. 1989. Epidemiological Studies on Campylobacter Enteritidis in Korea. 2. Antimicrobial Susceptibility and Plasmid Patterns of Campylobacter jejuni isolated from Animals. Kor. J. Vet. Publ. Hlth. 13, 105-114
  13. Karmali, M. A., S. D. Grandis and P. C. Fleming. 1981. Antimicrobial susceptibility of Campylobacter jejuni with special reference patterns of canadian isolates. Antimicrob. Agents Chemother. 15, 593-597
  14. Lior, H., D. L. Woodward, J. A. Edgar, L. J. Laroche and P. Gill. 1982. Serotyping of Campylobacter jejuni by slide agglutination based on heat-labile antigenic factors. J. Clin. Microbiol. 15, 761-768
  15. Llovo, J., E. Mateo, A. Munoz, M. Urquijo, L. W. On Stephen and F. A. Aurora. 2003. Molecular Typing of Campylobacter jejuni Involved in a Neonatal Outbreak Indicates Nosocomial Transmission. J. Clin. Microbiol. 41, 3926-3928 https://doi.org/10.1128/JCM.41.8.3926-3928.2003
  16. Luber, P., E. Bartelt, E. Genschow, J. Wagner and H. Hahn. 2003. Comparison of Broth Microdilution, E Test, and Agar Dilution Methods for Antibiotic Susceptibility Testing of Campylobacter jejuni and Campylobacter coli. J. Clin. Microbiol. 41, 1062-1068 https://doi.org/10.1128/JCM.41.3.1062-1068.2003
  17. Nylen, G., F. Dunstan, S. R. Palmer, Y. Andersson, F. Bager, J. Cowden, G. Feierl, Y. Gelloway, G. Kapperud, F. Megraud, K. Molbak, L. R. Petersen and P. Ruutu. 2002. The seasonal distribution of campylobacter infection in nine European countries and New Zealand. Epidermiol. Infect. 128, 187-192
  18. Owen, R. j., K. Sutherland, C. Fitzgerald, J. Gibson, P. Borman and J. Stanley. 1995. Molecular Subtyping Scheme Serotypes HS1 and HS4 of Campylobacter jejuni. J. Clin. Microbiol. 33, 872-877
  19. Oza, A. N., R. T. Thwaites, D. R. A. Wareing, F. J. Bolton and J. A. Forst. 2002. Detection of Heat-Stable Antigens of Campylobacter jejuni and C. coli by Direct Agglutination and Passive Hemagglutination. J. Clin. Microbiol. 40, 996-1000 https://doi.org/10.1128/JCM.40.3.996-1000.2002
  20. Penner, J. I. and J. N. Hennessy. 1980. Passive hemagglutination technique for serotyping Campylobacter fetus subsp. jejuni on the basis of soluble heat-stable antigens. J. Clin. Microbiol. 12, 732-737
  21. Rajan, D. P. and V. L. Mathan. 1982. Prevalence of Campylobacter fetus subsp. jejuni in healthy populations in southern India. J. Clin. Microbiol. 15, 749-751
  22. Skirrow, M. B. 1977. Campylobacter enteritis, A new disease. Br. Bed. J. 2, 9-11
  23. Takahashi, M., Koga Michiaki, K. Yokohama and N. Yuki. 2005. Epidemiology of Campylobacter jejuni Isolated from Patients with Guillain-Barre' and Fisher Syndromes in Japan. J. Clin. Microbiol. 43, 335-339 https://doi.org/10.1128/JCM.43.1.335-339.2005
  24. Woo, G. J., D. H. Lee, S. H. Park, Y. S. Kang, J. S. Park, H. S. Kwak, S. H. Lee, W. Y. Lee, Y. C. Park, Y. S. Cho, C. M. Kim, J. G. Chung, Y. S. Hu, S. J. Lee and S. H. Bing. 2002. Survey of food-borne pathogenic bacteria from diarrheal patients. The Annual Report of KFDA 6, 53-63

피인용 문헌

  1. Genetic Properties and Antimicrobial Resistance of Campylobacter jejuni Isolates from Diarrhea Patients in Gyeonggi-do vol.49, pp.3, 2013, https://doi.org/10.7845/kjm.2013.3030
  2. Clinical Guideline for the Diagnosis and Treatment of Gastrointestinal Infections vol.42, pp.6, 2010, https://doi.org/10.3947/ic.2010.42.6.323