Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Optimal Culture Conditions on the Tyrosinase Inhibitor Production by Actinomycetes F-97

방선균 F-97에 의한 Tyrosinase 저해제 생성 최적 배양 조건

  • Bang, Byung-Ho (Division of Food Science, Eulji Univesity) ;
  • Rhee, Moon-Soo (Korea Research Institute of Bioscience and Biotechnology) ;
  • Kim, Jin-O (Department of Microbial Engineering, Konkuk University) ;
  • Yi, Dong-Heui (Department of Microbial Engineering, Konkuk University)
  • 방병호 (을지대학교 식품과학부) ;
  • 이문수 (한국생명공학연구원) ;
  • 김진오 (건국대학교 미생물공학과) ;
  • 이동희 (건국대학교 미생물공학과)
  • Published : 2007.06.25
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Abstract

A Actinomycetes F-97 producing tyrosinase inhibitor was isolated from soil samples. The optimum culture condition for 쇼rosinase inhibitor production was investigated and the results were as follows. The best carbon source for tyrosinase inhibitor production was shown as soluble starch, the optimum concentration was 3.0%. The best nitrogen source for tyrosinase inhibitor production was shown as peptone, the optimum concentration was 0.36%. As effect of metal ions on the production of tyrosinase inhibitor, K$_2$HPO$_4$ was shown the best and the optimum concentration was 0.1 mM. The optimum pH and temperature was shown 7.0 and 30${\circ}$C, respectively. And the highest tyrosinase inhibitor production was observed at 70hr cultivation under optimum conditions in jar fermentor scale.

토양으로부터 tyrosinase 저해제를 생산하는 방선균 F-97을 분리하여 이 균주로부터 tyrosinase 저해제 생산을 위한최적 조건을 검토하였다. 그 결과는 다음과 같다. 탄소원으로는 soluble starch가 가장 좋았으며, 그 최적 농도는 3.0%였다. 질소원으로는 유기질소원인 peptone이 가장 좋았으며 최적 농도는 0.36%로 나타났다. 무기염으로 K$_2$HPO$_4$가 가장 좋았으며 최적농도는 0.1 mM이었다. 최적온도 30${\circ}$C와70시간의 jar fermentor 내에서 배양 시 최고의 tyrosinase 저해제생산성을 나타내었다.

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References

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