Korean Journal of Medicinal Crop Science (한국약용작물학회지)

The Korean Society of Medicinal Crop Science (한국약용작물학회)
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Effect of Plant Growth Regulators, Media and Celling Agents on In Vitro Microtuber Production of Pinellia ternata Breit

1식물생장조절제, 배지와 고형지지물이 반하의 기내 소괴경 생산에 미치는 영향

  • Kim, Yong-Kyung (Division of Plant Science & Resources, Chungnam National Uniersity) ;
  • Lee, Ji-Yeon (School of Bioscience & Biotechnology, Kangwon National University) ;
  • Kim, Eung-Hwi (Division of Plant Science & Resources, Chungnam National Uniersity) ;
  • Cho, Dong-Ha (School of Bioscience & Biotechnology, Kangwon National University) ;
  • Park, Sang-Un (Division of Plant Science & Resources, Chungnam National Uniersity)
  • Published : 2007.06.30
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Abstract

The study was carried out to establish in vitro microtuber production from leaf and petiole explant cultures of Pinellia ternata. Culture conditions were optimized by investigating the effect of plant growth regulators, different media, and gelling agents on the efficiency of microtuber indurtion. Among the different combinations of plant growth regulators tested, the combination of 0.1 mg/l 2,4-Dichlorophenoxyacetic (2,4-D) and 0.5 mg/l 6-benzylaminopurine (BAP) showed the highest yield fer microtuber production from leaf (3.9) and petiole (4.7) explant culture on MS medium far 6 weeks. SH(Schenk and Hildebrandt) medium was the most effective medium for microtuber induction and the half strength SH medium was better than SH medium for microtuber production from both leaf and petiole culture. Gelrite was better than agar in the formation of microtubers and 4% Gelrite showed the highest number of microtubers per explant frome leaf (5.9) and petiole (7.8) culture. Germination rate of microtubers after cold storaged for one months long was 86% from in vitro culture and 43% from autoclaved soil.

반하의 기내 조직배양을 통해 소괴경 생산을 하고자 적합한 식물생장조절제 처리조건, 배지와 처리 농도, 고형지지물의 종류와 농도를 조사하였다. 기내 소괴경 형성에 적합한 식물생장조절물질과 농도 조건을 연구 한 결과 0.1mg/l 2,4-D와 0.5 mg/l BAP 혼합처리에서 잎 (3.9개)과 엽병 (4.7개) 배양모두 최고치의 소괴경을 생산할 수 있었다. 기내 소괴경 형성에 적합한 배지는 SH배지로 나타났으며 잎과 엽병 배양에서 4.5와 5.2개의 소괴경을 생산하였으며, 최적 농도는 SH 배지의 무기염류를 반으로 줄인 1/2 SH배지가 가장 효과적이었다. 기내 소괴경 생산에 적합한 고형지지물과 그 농도를 조사한 결과, 잎과 엽병 배양 모두 agar보다 gelrite에서 소괴경 형성이 잘 되었으며, 농도별로는 agar 6%, Gelrite는 4% 처리에서최고치의 소괴경을 생산할 수 있었다. 기내에서 생산된 소괴경은 5$^{\circ}$C에서 1개월간 냉장 보관 후에 생장상에서 MS 고형배지가 든 배양용기와 멸균 소독한 상토에 옮겨 발아율을 조사한 결과, MS고형 배지에서 자란 소괴경은 86%의 발아율을 보였으며, 멸균 소독한 상토에서는 43%의 발아율을 보였다. 추후 반하 소괴경의 발아율과 토양 적응성을 높이는 연구보강으로 소경을 번식용 종자로 활용할 수 있는 방안을 모색할 예정이다.

Keywords

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