YAKHAK HOEJI (약학회지)

The Pharmaceutical Society of Korea (대한약학회)
권호 표지

Inhibitory Effects of Ethaverine on Serotonin Content in Murine Mastocytoma P815 Cells

Ethaverine 화합물이 P815 세포중의 Serotonin 함량에 미치는 영향

  • Kim, Eung-Il (College of Pharmacy, and Research Center for Bioresource and Health, Chungbuk National University) ;
  • Shin, Jung-Soo (College of Pharmacy, and Research Center for Bioresource and Health, Chungbuk National University) ;
  • Lim, Sung-Cil (College of Pharmacy, and Research Center for Bioresource and Health, Chungbuk National University) ;
  • Park, Seung-Kook (College of Pharmacy, and Research Center for Bioresource and Health, Chungbuk National University) ;
  • Lee, Myung-Koo (College of Pharmacy, and Research Center for Bioresource and Health, Chungbuk National University)
  • 김응일 (충북대학교 약학대학/생물건강산업개발연구센터) ;
  • 신정수 (충북대학교 약학대학/생물건강산업개발연구센터) ;
  • 임성실 (충북대학교 약학대학/생물건강산업개발연구센터) ;
  • 박승국 (충북대학교 약학대학/생물건강산업개발연구센터) ;
  • 이명구 (충북대학교 약학대학/생물건강산업개발연구센터)
  • Published : 2007.04.30
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Abstract

The effects of ethaverine on serotonin biosynthesis in murine mastocytoma P815 cells were investigated. Ethaverine at 2.5${\sim}$10 ${\mu}$M decreased serotonin content in a concentration-dependent manner. The IC$_{50}$ value of ethaverine was 6.1 ${\mu}$M. Ethaverine at concentrations up to 20 ${\mu}$M was not cytotoxic towards P815 cells. Under these conditions, tryptophan hydroxylase (EC 1.14.16.4; TPH), a rate-limiting enzyme in serotonin biosynthesis, was inhibited by ethaverine in P815 cells (15.3% inhibition at 7.5 ${\mu}$M), however, aromatic L-amino acid decarboxylase (EC 4.1.1.28) was not. These results indicate that ethaverine leads to a decrease in serotonin content by reducing TPH activity in P815 cells.

Keywords

References

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