Apoptosis of Human Lung Carcinoma Cells through the Inhibition of Bcl-2 Expression and Activation of Caspase by Chungjogupae-tang

인체폐암세포에서 Bcl-2 발현저하 및 caspase 활성을 통한 청조구폐탕의 apoptosis 유발에 관한 연구

  • Cho, In-Joo (Department of Oriental Medicine, Graduate School, Dong-Eui University) ;
  • Gam, Chul-Woo (Department of Oriental Medicine, Graduate School, Dong-Eui University) ;
  • Kim, Ki-Tak (Department of Oriental Medicine, Graduate School, Dong-Eui University) ;
  • Park, Dong-Il (Department of Oriental Medicine, Graduate School, Dong-Eui University)
  • 조인주 (동의대학교 한방내과학교실) ;
  • 감철우 (동의대학교 한방내과학교실) ;
  • 김기탁 (동의대학교 한방내과학교실) ;
  • 박동일 (동의대학교 한방내과학교실)
  • Published : 2007.02.25

Abstract

We previously reported the anti-proliferative effect of Chungjogupae-tang (CJGPT) in human lung carcinoma A549 cells, which was associated with the induction of cyclin-dependent kinase inhibitor p21 in a tumor suppressor p53-independent manner. CJGPT treatment also resulted in the inhibition of prostaglandin E2 release A549 cells by the down-regulation of cyclooxygenase-2. In the present study, we investigated the pathway of the induction of apoptotic cell death by CJGPT in A549 cells. It was found that CJGPT could inhibit the cell viability and induce the apoptotic cell death of A549 cells in a dose-dependent manner as measured by hemocytometer counts, flow cytometry analysis and agarose gel electrophoresis. Apoptosis of A549 cells by CJGPT was associated with a down-regulation of anti-apoptotic Bcl-2 and inhibitor of apoptosis proteins (IAPs) expression. Additionally, DNA fragmentation by CJGPT was connected with the activation of inhibitor of caspase-activated DNase/DNA fragmentation factor 45 (ICAD/DFF45) protein expression.

Keywords

References

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