DOI QR코드

DOI QR Code

Anti-Salmonella gallinarum Immunoglobulin 생산을 위한 계란의 이용

Effect of Charcoal on the Production of Anti-Salmonella gallinarum IgY

  • Rho, Jeong-Hae (Ginseng Research Group & Korea Food Research Institute) ;
  • Kim, Mi-Hyun (Ginseng Research Group & Korea Food Research Institute) ;
  • Kim, Young-Boong (Ginseng Research Group & Korea Food Research Institute) ;
  • Jung, Soon-Hee (Department of Food Science & Biotechnology, Sungkyunkwan University)
  • 발행 : 2007.06.30

초록

주요 가금티푸스 유발균인 Salmonella gallinarum에 대항하는 항체를 계란으로부터 효율적으로 얻기 위하여 사료 첨가제, 산란계에 백신 주사 시 adjuvant 종류, target균 외의 첨가균의 여부에 따른 계란 내의 면역 단백질 생산을 비교 측정하였다. 참숯의 급여에 의한 면역처리구의 specific IgY 함량을 보면 0.5% 참숯을 급여한 처리구가 가장 높은 함량을 나타내었으며, specific IgY의 생산에 참숯의 급여가 큰 영향을 미치는 것으로 나타났다. Adjuvant 처리에 따른 total IgY 함량의 변화는 거의 없는 것으로 나타났으나 specific IgY는 Freund's adjuvant를 사용하여 백신 처리한 처리구에서 높게 나타났다. Adjuvant 사용 시 결핵균을 같이 첨가한 경우 더 많은 specific IgY가 생성되었으나, 수산화 알미늄 처리구에서는 결핵균 첨가에 의한 영향이 나타나지 않았다. 15주령에 면역처리를 해준 경우 21주령에 면역처리를 해준 경우 보다 산란율이 높았으며, 사료에 참숯을 첨가한 경우 일반사료 급여군에 비해 산란율저하가 완화되었다.

In order to produce the antibody rich eggs against Salmonella gallinarum(S.G.) causing fowl typhoid, the productions of immunoglobulin in eggs were compared and examined with the feed additives, the variety of adjuvants in vaccines to layers, and the existence of additive antigens other than target microorganism. The examination of the average contents of specific IgY in immunized group by supplying hardwood charcoal showed that the group supplied with 0.5% hardwood charcoal had the highest contents, implying that the supply of hardwood charcoal promoted the production of specific IgY. Adjuvant appeared to have little effect on the average contents of total IgY, but specific IgY contents increased in the immunized group with Freund's adjuvant. Addition of BCG in adjuvant treatment increased specific IgY however, this feature was not seen in aluminum hydroxide treated group. Immunization at 15 week layers resulted in higher laying rate than immunization at 21 week and addition of hardwood charcoal in feed recovered laying rate. It was therefore, concluded that the feed supplement, such as hardwood charcoal followed by a proper immunization program concerning adjuvant, vaccination period and supplementary microorganism hastened the production of IgY.

키워드

참고문헌

  1. Gutierrez, M. A., Miyazaki, T., Hatai, H., and Kim, M. (1993) Protective properties of egg yolk IgY containing anti-Edwardsiella tarda aninck & Schlegel. J. Fish Diseases 16, 113-120 https://doi.org/10.1111/j.1365-2761.1993.tb00854.x
  2. Hamada, S., Horikoshi, T., Minami, T., Kawabata, S., Hiraoka, J., Fujiwara, T., and Ooshima, T. (1991) Oral passive immunization against dental caries in rats by use of hen egg yolk antibodies specific for cell-associated glucosyl-transferase of Streptococcus mutans. Infect. Immun. 59, 4161-4167
  3. Hatta, H., Kim. M. and Yamamoto T. (1990) A novel isolation method for hen egg yolk antibody 'IgY'. Agric. Biol. Chem. 54,2531-2535 https://doi.org/10.1271/bbb1961.54.2531
  4. Kim, Y. B., Rho, J. H., Shon, D. H., Kim, J. H., Sung, K. S., and Lee, N. H. (2000) Antimicrobial activity of specific IgY against strepteococcus mutans. Kor J. Food Sci. Technol. 32, 1319-1325
  5. Larsson, A., Balow, R. M., Linda, T. L., and Forsberg, R O. (1995) Chicken antibodies ; Taking advantage of evolution -A review. Poult. Sci. 72, 1807-1812
  6. Lee, K., Ametani, A., Shimizu, M., Hatta, H., Yamamoto, T., and Kimnogawa, S. (1991) Production and characterization of anti-human insulin antibodies in the hen's egg. Agric. Biol. Chem. 55, 2141-2143 https://doi.org/10.1271/bbb1961.55.2141
  7. Lee, N. H., Rho, J. H., Han, C. K., and Sung, K. S. (1999) Effect of various hen feed supplements on IgY level in eggs and laying rates. Kor. J. Anim. Sci. 41, 155-166
  8. Rho, J. H., Kim, Y B., Han, C. K., Lee, N. H., Sung, K. S., and Shon, D. H. (1999) The effect of age of hens and vaccin-cation on anti- strepteococcus mutans IgY level. Kor. J. Anim. Sci. 41, 563-574
  9. Rho, J. H., Kim, M. H., Kim, Y. B., Sung, K. S., and Lee, N. H. (2005) Formation and processing properties of anti-Salmonella gallinarum specific IgY from yolk. Kor. J. Anim. Sci. 47, 637-646 https://doi.org/10.5187/JAST.2005.47.4.637
  10. SAS. (1999) SAS/STAT Software for PC. Release 6.11, SAS institute, Cary, NC, U.S.A
  11. Shimizu, M., Fitzsimmons, R. C, and Nakai, S. (1998) Anti - E. coli immunoglobulin Y isolated from egg yolk immunized chickens as apotential food ingredient J. Food Sci. 53, 1360-1366 https://doi.org/10.1111/j.1365-2621.1988.tb09277.x
  12. Shon, D. H., Rho, J. H., Kim, Y B., Han, C. K. Sung, K. S., and Lee, N. H. (1998) Properties of anti-S.mutans IgY separated form egg yolk. Kor. J. Food Sci. Technol. 30, 1029-1034
  13. Yolken, R. H., Leister, F., Wee, S.-B., Miskuff, B., and Vonderfecht, S. (1998) Antibodies to rotavirus in chickens' eggs : A potential source of antiviral immunoglobulins suitable for human consumption. Pediatrics 81, 291-295
  14. 김영붕, 노정해, 한찬규, 성기승, 손동화, 김영동, 이성희, 허강준 (2001) 넙치의 질병 예방 치료를 위한 항생제 대용 특수면역 단백질 개발. 해양수산부 pp. 66-104
  15. 나만체 (1999) 국내 산란계에서 다발하는 주요 질병 및 대책 . 월간양계 2, 85-89
  16. 박경윤 (1995) 양계장의 살모넬라 감염실태와 대책. 양계연구 6, 54-58
  17. 오경록 (1999) 가금용 살모넬라균 백신의 장단점. 월간양계 1, 52-53
  18. 이성춘 (2004) 참숯과 한약재를 이용한 닭 사료. 대한민국특허청 10-2004-0064557
  19. 정운익 (1993) 훌륭한 면역항체 공급원 계란 난황속의 항체. 양계연구 pp. 40-42