Journal of Periodontal and Implant Science

  • 제37권1호
  • /
  • Pages.45-51
  • /
  • 2007
  • /
  • 2093-2278(pISSN)
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  • 2093-2286(eISSN)
대한치주과학회 (Korean Academy of Periodontology)
권호 표지

치주염에 이환되어 발거된 치아의 치주인대가 없는 치근표면에 EDTA처리를 한 후 3차원적으로 배양한 사람치주인대섬유모세포의 부착에 관한 주사전자현미경적 연구

The 3-dimensional attachment of human periodontal ligament fibroblasts on periodontally involved root surface following treatment with EDTA: A SEM study in vitro

  • 조아영 (조선대학교 치과대학 치주과학 교실) ;
  • 김병옥 (조선대학교 치과대학 치주과학 교실, 구강생물학연구소) ;
  • 박주철 (조선대학교 치과대학 구강조직학 교실, 구강생물학연구소) ;
  • 김흥중 (조선대학교 치과대학 구강해부학 교실, 구강생물학연구소) ;
  • 장현선 (조선대학교 치과대학 치주과학 교실, 구강생물학연구소)
  • cho, Ah-Young (Department of Periodontology, College of Dentistry, Chosun University) ;
  • Kim, Byung-Ock (Department of Periodontology, College of Dentistry, Chosun University, Oral Biology Research Institute) ;
  • Park, Joo-Cheol (Department of Oral Histology, College of Dentistry, Chosun University, Oral Biology Research Institute) ;
  • Kim, Heung-Jeong (Department of Oral Anatomy, College of Dentistry, Chosun University, Oral Biology Research Institute) ;
  • Jang, Hyun-Seon (Department of Periodontology, College of Dentistry, Chosun University, Oral Biology Research Institute)
  • 발행 : 2007.03.30
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초록

This study evaluated the possibility of the 3-dimensional attachment of human periodontal ligament fibroblasts to a periodntally involved root surface after an EDTA treatment in vitro. The human PDL fibroblasts were isolated from the middle third of the root of periodontally healthy teeth extracted for orthodontic reasons. The cells were cultured in a medium containing Dulbecco's modified Eagle medium supplemented with 10% fetal bovine serum at $37^{\circ}C$ in humidified air containing 5% $CO_2$. Eight single-rooted teeth were obtained from patients diagnosed with periodotitis. After scaling and root planing, four teeth were etched with 24% ethylenediaminetetracetic acid (EDTA) for two minutes (Experimental group). The other four teeth were not treated with EDTA and were used as the control group. The human PDL fibroblasts were placed in the total root surface and cultured for 4 weeks. The teeth were fixed in 2.5% glutaraldehyde in PBS before preparation for the scanning electron microscopy (SEM) examination. The human PDL fibroblasts showed a healthy morphology on the root surfaces treated with EDTA (Experimental group) and a relatively unhealthy appearance on the treated root surfaces (Control group). This suggests that EDTA favorably affects the 3-dimensional attachment of human PDL fibroblasts cultured on the root surfaces. which may play an important role in periodontal healing and regeneration.

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