Journal of Periodontal and Implant Science

  • 제37권1호
  • /
  • Pages.35-44
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  • 2007
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  • 2093-2278(pISSN)
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  • 2093-2286(eISSN)
대한치주과학회 (Korean Academy of Periodontology)
권호 표지

PDLs22 재조합 단백질의 합성과 평가

Synthesis and evaluation of PDLs22 recombinant protein

  • 이경연 (조선대학교 구강생물학 연구소 및 2단계 BK21) ;
  • 최용석 (조선대학교 구강생물학 연구소 및 2단계 BK21) ;
  • 이유진 (조선대학교 구강생물학 연구소 및 2단계 BK21) ;
  • 배현숙 (남서울대학교 치위생학과) ;
  • 김흥중 (조선대학교 구강생물학 연구소 및 2단계 BK21) ;
  • 조광희 (조선대학교 구강생물학 연구소 및 2단계 BK21) ;
  • 장현선 (조선대학교 구강생물학 연구소 및 2단계 BK21) ;
  • 박주철 (조선대학교 구강생물학 연구소 및 2단계 BK21)
  • Lee, Kyoung Yeon (Oral Biology Research Institute, Chosun University and the second stage of BK21) ;
  • Choi, Yong-Seok (Oral Biology Research Institute, Chosun University and the second stage of BK21) ;
  • Lee, You-Jin (Oral Biology Research Institute, Chosun University and the second stage of BK21) ;
  • Bae, Hyun-Sook (Dept. of Dental Hygiene, Namseoul University) ;
  • Kim, Heung-Jeong (Oral Biology Research Institute, Chosun University and the second stage of BK21) ;
  • Cho, Kwang-Hee (Oral Biology Research Institute, Chosun University and the second stage of BK21) ;
  • Jang, Hyun-Seon (Oral Biology Research Institute, Chosun University and the second stage of BK21) ;
  • Park, Joo-Cheol (Oral Biology Research Institute, Chosun University and the second stage of BK21)
  • 발행 : 2007.03.30
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초록

Periodontal ligament (PDL) is the connective tissue located between the tooth root and alveolar bone. In a previous study, PDLs22 was isolated as a PDL-specific gene by using subtractive hybrid-ization between cultured PDL fibroblasts and gingival fibroblasts. It was also suggested that PDLs22 plays important roles in the development, differentiation and maintenance of periodontal tissues. However, little is known about functional study of PDLs22 using recombinant protein in PDL fibroblast differentiation and periodontium formation. In this study, in order to produce the PDLs22 recombinat protein, PDLs22 expression vector were constructed and expressed its protein in various host cell and temperature conditions. The results were as follows: 1. PDLs22 protein was not strongly expressed In the induction system using pRSET-PDLs22 construct. 2. When the BL21(DE3) pLysS was used as a expression host, PDLS22 protein was strongly ex-pressed in the induction system using pHCEIIBNd-PDLs22 construct. 3. The PDLs22 protein was recognized at a molecular weight of 28 kDa in western blots. 4. Almost of the expressed PDLs22 protein was not soluble and observed like as inclusion body. 5. The protein solubility was not improved after modification of induction time and temperature during PDLs22 protein production. In this study, the system for the PDLs22 protein production was connstructed. However, the re-results suggest that further studies will be needed to produce the considerable amount of PDLs22 re-combinat protein, which can use for the periodontal regeneration.

키워드

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