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Antioxidant Activity of Heated Licorice (Glycyrrhiza uralensis Fisch) Extracts in Korea

열처리한 국산 감초추출물의 항산화활성

  • Woo, Koan-Sik (Dept. of Food Science and Technology, Chungbuk National University) ;
  • Hwang, In-Guk (Dept. of Food Science and Technology, Chungbuk National University) ;
  • Noh, Young-Hee (Dept. of Beauty Industry, Konyang University) ;
  • Jeong, Heon-Sang (Dept. of Food Science and Technology, Chungbuk National University)
  • Published : 2007.06.30

Abstract

Antioxidative activity and polyphenol contents of heated licorice in Korea extracted by ethyl.acetate (EtOAc) and ethyl.alcohol (EtOH) were evaluated at various heating temperatures (110, 120, 130, 140, and $150^{\circ}C$), times (1, 2, 3, 4, and 5 hr), and moisture contents (10, 20, 30, 40, and 50%). Maximum extraction yields of EtOAc extract was 10.9% at $130^{\circ}C$, 3 hr, and 50% moisture content and that of EtOH extract was 25.0% at $120^{\circ}C$, 2 hr, and 20% moisture content, whereas those of control were 0.8 and 15.8%, respectively. The highest total polyphenol content was 845.67 mg/100 g in EtOH extract at $120^{\circ}C$, 2 hr, and 20% moisture content (control: 277.00 mg/100 g). The antioxidative activity ($IC_{50}$) was the highest value of 0.53 mg/mL in EtOAc extract at $120^{\circ}C$, 2 hr, and 20% moisture content (control: 12.34 mg/mL). The highest ascorbic acid equivalent antioxidant activity value of 1,584 mg ascorbic acid (AA) eq was obtained from EtOAc extract at $120^{\circ}C$, 2hr, and 40% moisture content (control: 1,263 mg AA eq). Optimum heating conditions for the improvement of antioxidative activity of licorice in Korea was $120^{\circ}C$, 2 hr, and $20{\sim}40%$ moisture content.

국산 감초를 처리온도, 시간 및 첨가수분함량을 변수로하여 열처리한 다음 에틸아세테이트와 에탄올로 추출하여 성분 및 생리활성 변화를 분석하고 반응표면분석으로 최적화 조건을 조사하였다. 에틸아세테이트 추출물의 추출수율은 $130^{\circ}C$, 3시간, 가수량 50%일 때 10.90%로 무처리구의 0.80%보다 높았으며, 에탄올 추출물은 $120^{\circ}C$, 2시간, 가수량 20%로 처리한 시료가 25.00%로 무처리구의 15.80%보다 높았다. 총 폴리페놀 함량은 열처리구가 무처리구보다 높았으며, 에탄올 추출물의 $120^{\circ}C$, 2시간, 가수량 20% 처리구가 845.67 mg/100 g으로 무처리구의 277.00 mg/100 g보다 높았다. 항산화활성($IC_{50}$)은 열처리구가 무처리구보다 높게 나타났으며, 에틸아세테이트 추출물 $120^{\circ}C$, 2시간, 가수량 20% 처리구에서 0.53 g/L로 무처리구의 12.34 g/L보다 매우 높게나타났다. 총항산화력(AEAC)은 모든 처리구에서 무처리구보다 높게 나타났는데 에틸아세테이트 추출물 $120^{\circ}C$, 2시간, 가수량 40% 처리구에서 1,584 mg AA eq로 무처리구의 1,263 mg AA eq보다 높게 나타났다. 본 연구결과 감초의 항산화활성, 총 폴리페놀 함량 등을 증가시키기 위한 최적의 열처리 조건은 $120^{\circ}C$, 2시간, 가수량 $20{\sim}40%$로 판단되었다.

Keywords

References

  1. Kang MH, Park CG, Cha MS, Seong NS, Chung HK, Lee JB. 2001. Component characteristics of each extract prepared by different extract methods from by-products of Glycyrrhizia uralensis. J Korean Soc Food Sci Nutr 30: 138-142
  2. Shibata S, Inoue H, Iwata S, Ma RD, Yu LJ, Ueyama U, Takayasu J, Hasegawa T, Tokyda H, Nishino A. 1991. Inhibitory effect of licochalcone a isolated from Glycyrrhiza inflata root on inflammatory ear edema and promotion in mice. J Planta Med 57: 221-224 https://doi.org/10.1055/s-2006-960078
  3. Hanato T, Aga Y, Shintani Y, Ito H, Okuda T, Yoshida T. 2000. Minor flavonoids from licorice. Phytochemistry 55: 959-963 https://doi.org/10.1016/S0031-9422(00)00244-2
  4. Vaya J, Belinky PA, Aviram M. 1997. Antioxidant constituents from licorice roots: isolation, structure elucidation and antioxidative capacity toward LDL oxidation. Free Radic Biol Med 23: 302-313 https://doi.org/10.1016/S0891-5849(97)00089-0
  5. Ishikawa S, Kato M, Tokuda T, Momoi H, Sekijima Y, Higuchi M, Yanagisawa N. 1999. Licence-induced hypokalemic myopathy and hypokalemic renal tubular damage in anorexia nervosa. Int J Eat Disord 26: 111-114 https://doi.org/10.1002/(SICI)1098-108X(199907)26:1<111::AID-EAT16>3.0.CO;2-U
  6. Choi Y, Lee SM, Chun J, Lee HB, Lee J. 2006. Influence of heat treatment on the antioxidant activities and polyphenolic compounds of Shiitake (Lentinus edodes) mushroom. Food Chem 99: 381-387 https://doi.org/10.1016/j.foodchem.2005.08.004
  7. Kim MR, Kim IH, Shim JH. 2005. The analysis of volatile components of fresh ginseng, red ginseng and white ginseng by solvent free solid injector (SFSI) techniques. Korean J Enviro Agr 24: 164-168 https://doi.org/10.5338/KJEA.2005.24.2.164
  8. Woo KS, Jang KI, Kim KY, Lee HB, Jeong HS. 2006. Antioxidative activity of heat treated licorice (Glycyrrhiza uralensis Fisch) extracts. Korean J Food Sci Technol 38: 355-360
  9. Kwon OC, Woo KS, Kim TM, Kim DJ, Hong JT, Jeong HS. 2006. Physicochemical characteristics of garlic (Allium sativum L.) on the high temperature and pressure treatment. Korean J Food Sci Technol 38: 331-336
  10. Hwang IG, Woo KS, Kim TM, Kim DJ, Yang MH, Jeong HS. 2006. Change of physicochemical characteristics of Korean pear (Pyrus pyrifolia Nakai) juice with heat treatment conditions. Korean J Food Sci Technol 38: 342-347
  11. Yang SJ, Woo KS, Yoo JS, Kang TS, Noh YH, Lee J, Jeong HS. Change of Korean ginseng components with high temperature and pressure treatment. Korean J Food Sci Technol 38: 521-525
  12. Park JH. 2004. Sun ginseng-a new processed ginseng with fortified activity. Food Ind Nutr 9: 23-27
  13. Dewanto V, Wu X, Adom KK, Liu RH. 2002. Thermal processing enhances the nutritional value of tomatoes by increasing total antioxidant activity. J Agric Food Chem 50: 3010-3014 https://doi.org/10.1021/jf0115589
  14. Dewanto V, Xianzhong W, Liu RH. 2002. Processed sweet corn has higher antioxidant activity. J Agric Food Chem 50: 4959-4964 https://doi.org/10.1021/jf0255937
  15. Jeong SM, Kim SY, Kim DR, Jo SC, Nam KC, Ahn DU, Lee SC. 2004. Effect of heat treatment on the antioxidant activity of extracts from citrus peels. J Agric Food Chem 52: 3389-3393 https://doi.org/10.1021/jf049899k
  16. Blois MS. 1958. Antioxidant determinations by the use of a stable free radical. Nature 26: 1199-1203 https://doi.org/10.1038/1811199a0
  17. Turkmen N, Sari F, Velioglu YS. 2005. The effect of cooking methods total phenolics and antioxidant activity of selected green vegetables. Food Chem 93: 713-718 https://doi.org/10.1016/j.foodchem.2004.12.038

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