Soil Mineral Nutrients and Microbes Are Responsible for Large Patch Disease Caused by Rhizoctonia solani AG2-2 in Zoysiagrass Turf

골프장 한국잔디의 Rhizoctonia solani AG2-2에 의한 Large Patch 발생 토양에서 근권 미생물과 무기영양 평가

  • Chang, Tae-Hyun (Department of Plant Resources, College of Life Science and Natural Resources, Sangju National University) ;
  • Ru, Yeon-Ju (Department of Bioindustry, College of Life and Environmental, Daegu University) ;
  • Lee, Yong-Se (Department of Bioindustry, College of Life and Environmental, Daegu University)
  • 장태현 (상주대학교 생명자원대학 식물자원학과) ;
  • 류연주 (대구대학교 생명환경대학 산업바이오) ;
  • 이용세 (대구대학교 생명환경대학 산업바이오)
  • Published : 2007.12.31

Abstract

Mineral nutrients and population dynamics of soil microbes in the root zones of zoysiagrass infected by Rhizoctonia solani AG2-2 and that of healthy plants were sampled from ten golf courses using a cup cutter(diameter $10\;cm\;{\times}\;8\;cm$ deep). Analysis of variance(ANOVA) showed significant differences in content of $NO_3$-N(P = 0.05), $NH_4$-N(P = 0.1), and K(P = 0.1) between infected and healthy samples. The content of $NO_3$-N in the soils of large patch was 9.49 mg/kg and that in soil of healthγ plants was 7.02 mg/kg. However, the content of $NH_4$-N in the soil of large patch was 12.02 mg/kg whereas 14.40 mg/kg for the soil under the healthy plants. The content of K in the soil of large patch was lower than that of soil of healthy plants. There was few numbers of Pseudomonas colonies In the soils of large patch compared to that of healthy plants. These results indicated that the content of $NO_3$-N, NH4-N, and K and the microbial population dynamics in root zones correlated to occurrence of large patch.

골프장의 한국 잔디에서 Rhizoctonia solani AG2-2에 의해 발생되는 large patch의 근권토양과 건전 잔디의 근귄 토양 샘플을 hole cutter (diameter $10\;cm\;{\times}\;8\;cm$ deep)을 이용하여 샘플을 채취하여 무기성분과 미생물을 조사하였다. Large patch와 건전 잔디의 분산분석 결과 $NO_3$-N(P=0.05), NH4-N(P=0.1) 및 K(P=0.1) 함량에서 중요한 유의차이를 보였다. Large patch가 발생한 토양에 $NO_3$-N 함량은 9.49 mg/kg로 건전한 잔디의 토양 7.02 mg/kg 보다 높았다. 반면, $NH_4$-N의 함량은 large patch 발생한 토양이 12.02 mg/kg으로 건전한 잔디의 근권 토양 14.40 mg/kg 보다 높았다. K 함량은 large patch 토양이 건전한 잔디의 토양보다 낮았다. 근권 토양의 미생물 집락 수를 조사하여 분산 분석한 결과 Pseudomonas spp 집락 수에서 중요한 유의차이(P=0.05)를 보였다. Large patch가 발생한 토양에 미생물의 집락 수가 건전한 잔디의 토양에 비하여 낮았다. 이들 결과는 근권 토양에 과다한 $NO_3$-N, $NH_4$-N 및 K 함량과 토양미생물의 밀도는 large patch 발병과 관련 이 있을 수 있다고 사료된다.

Keywords

References

  1. Beevers, H. 1961. Respiratory metabolism in plants. Evanston, III.: Row, Peterson. 232pp
  2. Couch, H.B. and Bloom, J.R. 1960. Influence of environment on diseases of turfgrasses. II: Effect of nutrition, pH, and soil moisture on Sclerotinia dollar spot. Phytopathology 50:761-763
  3. Couch, H.B. 2000. The turfgrass disease handbook. Academic press Inc. pp.11-67
  4. Dale, J.L. 1978. Atypical symptoms of rhizoctonia infection on Zoysia grass. Plant Dis. Rep. 62: 645-647
  5. Huber, D.M. 1980. The role of nutrition in defence. Academic Press, N.Y. Pages 381-406
  6. Huber, D.M. and Waston, R.D. 1974. Nitrogen form and plant disease. Annu. Rev. Phytopathol. 12:139-165 https://doi.org/10.1146/annurev.py.12.090174.001035
  7. Huber, D.M., Painter, C.G., Mckay, H.C. and Peterson, D.L. 1968. Effects of nitrogen fertilization on take-all of winter wheat. Phytopathology 60:22-26 https://doi.org/10.1094/Phyto-60-22
  8. Hayns, RL. 1986. Mineral nitrogen in the plant-soil system. Academic press Inc. pp. 20-50
  9. Keim, R, and Humphrey, W.A. 1984. Fertilizer helps control Fusarium wilt of Hebe. Calif. Agric, 38:12-14
  10. NIAST. 2000. Methods of soil and plant analysis. National Institute of Agricultural Science and Technology, RDA, Suwon, Korea
  11. Shim, G.Y., Kim, J.W. and Kim, H.K. 1994. Occurrence of Rhizoctonia blight of zoysiagrasses in golf courses in Korea. Korean Journal of Plant Pathology 10(1):54-60
  12. Singh, D., Brinkerhoff, L.A. and Guinn, G. 1971. Effect of alanine on development of verticillium wilt in cotton cultivars with different levels of resistance. Phytopathology 61:881-882 https://doi.org/10.1094/Phyto-61-881
  13. Smiley, R.W., Cook, R.J. and Pappendick, R.I. 1970. Anhydrous ammonia as a soil fungicide againt Fusarium and fungicidal activity in the ammonia retention zone. Phytopathology 60:1227-1232 https://doi.org/10.1094/Phyto-60-1227
  14. Smiley, R.W., Cook, R.J. and Pappendick, R.I. 1972. Fusarium foot rot of wheat and peas as influenced by soil applications of anhydrous ammonia and ammonia potassium azide solutions. Phytopathology 62:86-91 https://doi.org/10.1094/Phyto-62-86
  15. Smiley, R.W. and Cook, R.J. 1973. Relationship between take-all of wheat and rhizosphere pH In soil fertilized with ammomum vs. nitrate-nitrogen. Phytopathology 63:882-889 https://doi.org/10.1094/Phyto-63-882
  16. Smiley, R.W., Dernoeden, P.H, and Clarke, B.B. 1992. Compendium of Turgrass Diseases. 2nd ed. American Phytopathological Society, St. Paul, MN
  17. Smith, J.D. 1957. The control of fusarium patch disease with fungicides. In the control of certain diseases of sports turfgrasses in the British Isles. M. Sc. Thesis, Univ. Durham 1:1-96
  18. Smith, J.D., Jackson, N., and Woolhouse, A.R 1989. Fungal Diseases of Amenity Turfgrass. London: E&F.N. Spon Ltd
  19. Spiegel, Y., and Netzer, D. 1984. Effects of nitrogen form at various levels of potassium on the Meloiogyne-Fusarium wilt complex in muskmelon. Plant and soil 81:85-92 https://doi.org/10.1007/BF02206897
  20. Tharp, W.H., and Wadleigh, C.H. 1939. The effects of nitrogen, phosphorus, and potassium nutrition on the Fusarium wilt of cotton. Phytopathology 29:756
  21. Walker, J.C. and Foster, R.E. 1946. Plant nutrition in relation to disease development. ill Fusarium wilt of tomato. Am. J. Bot. 33:259-264 https://doi.org/10.2307/2437431
  22. Webster, G.C. 1959. Nitrogen metabolism in plants. Evanston, III.:Row. Peterson. 152pp
  23. Willis, C.B. 1968. Effect of various nitrogen sources on growth of Sclerotinia. Can. J. Microbiol. 14:1035-1037 https://doi.org/10.1139/m68-174
  24. Woltz, S.S., and Jones, J.P. 1973. Interactions in source of nitrogen fertilizer and liming procedure in the control of Fusarium wilt of tomato. Hort Science 8:137-138