Journal of Microbiology and Biotechnology

  • 제17권5호
  • /
  • Pages.847-852
  • /
  • 2007
  • /
  • 1017-7825(pISSN)
  • /
  • 1738-8872(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
권호 표지

An Efficient Method for the Extraction of Astaxanthin from the Red Yeast Xanthophyllomyces dendrorhous

  • Choi, Seok-Keun (Department of Biotechnology, School of Life Sciences and Biotechnology, Korea University) ;
  • Kim, Jeong-Hwan (Department of Physiology, College of Medicine, Pusan National University) ;
  • Park, Young-Sam (Department of Biotechnology, School of Life Sciences and Biotechnology, Korea University) ;
  • Kim, Young-Jin (Korea Food Research Institute) ;
  • Chang, Hyo-Ihl (Department of Biotechnology, School of Life Sciences and Biotechnology, Korea University)
  • 발행 : 2007.05.31
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

This study investigated an efficient method for the extraction of astaxanthin from the red yeast Xanthophyllomyces dendrorhous. The extraction process comprised three steps: 1) cultivating the yeast; 2) treating the yeast culture suspension with microwaves to destroy the cell walls and microbodies; and 3) drying the yeast and extracting the astaxanthin pigment using ethanol, methanol, acetone, or a mixture of the three as the extraction solvent. Ultimately, various treatment tests were performed to determine the conditions for optimal pigment extraction, and the total carotenoid and astaxanthin contents were quantified. A frequency of 2,450 MHz, an output of 500 watts, and irradiation time of 60 s were the most optimum conditions for yeast cell wall destruction. Furthermore, optimal pigment extraction occurred when using a cell density of 10g/l at $30^{\circ}C$ over 24 h, with a 10% volume of ethanol.

키워드

참고문헌

  1. An, G. H., D. B. Schuman, and E. A. Johnson. 1989. Isolation of Phaffia rhodozyma mutants with increased astaxanthin contents. Appl. Environ. Microbiol. 55: 116- 124
  2. Bendich, A. and J. A. Olson. 1989. Biological actions of carotenoids. FASEB J. 3: 1927-1932 https://doi.org/10.1096/fasebj.3.8.2656356
  3. Britton, G. 1967. General carotenoid methods. J. Chem. Soc. Chem. Commun. 3: 49
  4. Foppen, F. H. 1971. Tables for the identification of carotenoid pigments. Chromtogr. Rev. 14: 113 https://doi.org/10.1007/BF02311200
  5. Fujikawa, H. and K. Ohta. 1994. Patterns of bacterial destruction in solution by microwave irradiation. Appl. Environ. Microbiol. 76: 389-394 https://doi.org/10.1111/j.1365-2672.1994.tb01645.x
  6. Fujikawa, H., H. Ushioda, and Y. Kudo. 1992. Kinetics of Escherichia coli destruction by microwave irradiation. Appl. Environ Microbiol. 58: 920-924
  7. Jin, E. S., J. W. Polle, H. K. Lee, S. M. Hyun, and M. Chang. 2003. Xanthophylls in microalgae: From biosynthesis to biotechnological mass production and allocation. J. Microbiol. Biotechnol. 13: 165-174
  8. Johnson, E. A. and G. H. An. 1991. Astaxanthin from microbial sources. Crit. Rev. Biotechnol. 11: 297-326 https://doi.org/10.3109/07388559109040622
  9. Johnson. E. A. and W. A. Schroeder. 1995. Microbial carotenoids. Adv. Biochem. Eng. 53: 119-178
  10. Johnson, E. A., D. E. Conklin, and M. J. Lewis. 1977. The yeast Phaffia rhodozyma as a dietary pigment source for salmonoids and crustaceans. J. Fish Res. Bd. Canada 34: 2417-2421 https://doi.org/10.1139/f77-327
  11. Johnson, E. A., T. G. Villa, M. J. Lewis, and H. J. Phaff. 1979. Lysis of the cell wall of the Yeast Phaffia rhodozyma by a lytic enzyme complex from Bacillus circulans WL-12. J. Appl. Biochem. 1: 273-282
  12. Jyonouchi, H., S. Sun, K. Lijima, and M. D. Gross. 2000. Antitumor activity of astaxanthin and its mode of action. Nutr. Cancer 36: 59-65 https://doi.org/10.1207/S15327914NC3601_9
  13. Kim, J. D., W. S. Lee, B. M. Kim, and C. G. Lee. 2006. Proteomic analysis of protein expression patterns associated with astaxanthin accumulation by green alga Haematococcus pluvialis (Chlorophyceae) under high light stress. J. Microbiol. Biotechnol. 16: 1222-1228
  14. Kim, J. H., C. W. Kim, and H. I. Chang. 2004. Screening and characterization of red yeast Xanthophyllomyces dendrorhous mutants. J. Microbiol. Biotechnol. 14: 570-575
  15. Kim, J. H. and H. I. Chang. 2006. High-level production of astaxanthin by Xanthophyllomyces dendrorhous mutant JH1, using chemical and light induction. J. Microbiol. Biotechnol. 16: 381-385
  16. Kim, J. H., S. K. Choi, W. J. Lim, and H. I. Chang. 2004. Protective effect of astaxanthin produced by Xanthophyllomyces dendrorhous mutant on indomethacin-induced gastric mucosal injury in rats. J. Microbiol. Biotechnol. 14: 996-1003
  17. Kim, J. H., S. K. Choi, Y. S. Park, C. W. Yun, W. D. Cho, K. M. Chee, and H. I. Chang. 2006. Effect of culture conditions on astaxanthin formation in red yeast Xanthophyllomyces dendrorhous mutant JH1. J. Microbiol. Biotechnol. 16: 438- 442
  18. Kim, S. J., G. J. Kim, D. H. Park, and Y. W. Ryu. 2003. High-level production of astaxanthin by fed-batch culture of mutant strain Phaffia rhodozyma AJ-6-1. J. Microbiol. Biotechnol. 13: 175-181
  19. Naguib, Y. M. 2000. Antioxidant activities of astaxanthin and related carotenoids. J. Agric. Food Chem. 48: 1150- 1154 https://doi.org/10.1021/jf991106k
  20. Sedmak, J. J., D. K. Weerasinghe, and S. O. Jolly. 1990. Extraction and quantitation of astaxanthin from Phaffia rhodozyma. Biotechnol. Tech. 4: 107-112 https://doi.org/10.1007/BF00163282
  21. Vecchi, M. and R. K. Muller. 1979. Separation of (3S, 3'S)-, (3R, 3'R)-, and (3S, 3S'R)-astaxanthin via (-)-camphanic acid esters. J. High Res. Chromatogr. Commun. 2: 195
  22. Yuan, J. P. and F. Chen. 1997. Identification of astaxanthin isomers in Haematococcus lacustris by HPLC-photodiode array detection. Biotechnol. Tech. 11: 445-459