Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Genetic Relationship and Characteristics Using Microsatellite DNA Loci in Horse Breeds.

Microsatellite DNA를 이용한 말 집단의 유전적 특성 및 유연 관계

  • Cho, Gil-Jae (College of Veterinary Medicine, Kyungpook National University)
  • Published : 2007.05.25
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Abstract

The present study was conducted to investigate the genetic characteristic and to establish the parentage verification system of the Korean native horse(KNH). A total number of 192 horses from six horse breeds including the KNH were genotyped using 17 microsatellite loci. This method consisted of multiplexing PCR procedure. The number of alleles per locus varied from 5 to 10 with a mean value of 7.35 in KNH. The expected heterozygosity and observed heterozygosity were ranged from 0.387 to 0.841(mean 0.702) and from 0.429 to 0.905(mean 0.703), respectively. The total exclusion probability of 17 microsatellite loci was 0.9999. Of the 17 markers, AHT4, AHT5, CA425, HMS2, HMS3, HTG10, LEX3 and VHL20 marker have relatively high PIC value(>0.7). This study found that there were specific alleles, P allele at AHT5, Q allele and R allele at ASB23, H allele at CA425, S allele at HMS3, J allele at HTG10 and J allele at LEX3 marker in KNH when compared with other horse populations. Also, the results showed two distinct clusters: the Korean native horse cluster(Korean native horse, Mongolian horse), and the European cluster(Jeju racing horse, Thoroughbred horse). These results present basic information for detecting the genetic markers of the KNH, and has high potential for parentage verification and individual identification of the KNH.

말 6개 품종 192두를 대상으로 17개의 microsatellite DNA marker를 이용하여 유전자(DNA)형을 분석하여 비교한 결과 제주마에서 각 marker별로 대립유전자의 수는 5-10개(평균 7.35개)로 분포하였고 제주마에서 관찰된 대립유전자는 총 125개가 관찰되어 평균 좌위 당 7.35개로서 몽고마의 130개(평균 7.65개)보다는 낮은 수치였다. 또한 AHT5 marker에서 대립유전자 P, ASB23 marker에서 대립유전자 Q와 R, CA425 marker에서 대립유전자 H, HMS3 marker에서 대립유전자 S, HTG10 marker에서 대립유전자 J, LEX3 marker에서 대립유전자 J 등 6개 marker에서 7개의 특이 대립유전자가 관찰되었다. 관찰된 이형접합성(observed heterozygosity)과 기대된 이형접합성(expected heterozygosity)은 각각 0.429-0.905(평균 0.703)와 0.387-0.841(평균 0.702)로 관찰되었고 다량정보량(PIC)은 0.354(HTG6)-0.816(LEX3)로서 평균 0.659로 나타났으며 17개 marker중 AHT4, AHT5, CA425, HMS2, HMS3, HTG10, LEX3, VHL20 marker 등이 다량정보량(PIC) 0.7 이상을 나타내었다. 17개 marker에 대한 전체 부권부정율(친부마 혹은 친모마 하나의 유전자형을 알고 있을 경우)을 제주마에 적용 시 99.99%로 나타났다. 말 6개 품종별로 분석하였을 때 평균 대립유전자의 수는 7.64개(몽고마)-4.23개(미니츄어 말)로 분포하였고 17개 marker 전체에서는 153개의 대립유전자가 검출되었다. 품종별로 분석한 결과 기대된 이형접합성(expected heterozygosity)과 관찰된 이형접합성(observed heterozygosity)은 각각 0.7950$\pm$0.0141(몽고마)-0.6751$\pm$0.0378(미니츄어 말), 0.7135$\pm$0.0180(제주경주마)-0.5621$\pm$0.0401(미니츄어 말)로 나타났다. 말 6개 품종을 17개 microsatellite marker로 분석한 결과 몽고마, 제주마, 제주경주마 등의 순으로 높은 유전적 다양성을 보였다. 제주마와 가장 가까운 유전적 유연 관계를 나타낸 집단은 몽고마로서 Da genetic distance에서 0.1517로 나타났고, 제주경주마와는 0.2628의 유전적 거리를 보였다.

Keywords

References

  1. Aranguren-Mendez, J., J. Jordana and M. Gomez. 2001. Genetic diversity in Spanish donkey breeds using micro-satellite DNA markers. Genet. Sel. Evol. 33, 433-442 https://doi.org/10.1186/1297-9686-33-4-433
  2. Binns, M. M., N. G. Uolmes and A. M. Holliman. 1995. The identification of polymorphic microsatellite loci in the horse and their use in thoroughbred parentage testing. Brit. Vet. J. 151, 9-15 https://doi.org/10.1016/S0007-1935(05)80057-0
  3. Bowling, A. T., M. L. Eggleston-Scott, G. Byrns, R. S. Clark, S. Dileanis and E. Wictum. 1997. Validation of microsatellite markers for routine horse parentage testing. Anim. Genet. 28, 247-252 https://doi.org/10.1111/j.1365-2052.1997.00123.x
  4. Bozzini, M., D. Fantin, J. S. Ziegle, H. van Haeringen, W. Jacobs, M. Ketcham, M. Spencer and S. Bates. 1996. Automated equine paternity testing. Anim. Genet. 27, 32
  5. Breen, M., G. Lindgren, M. M. Binns, J. Norman, Z. Irvin, K. Bell, K. Sandberg and H. Ellegren. 1997. Genetical and physical assignments of equine microsatellites-first integration of anchored markers in horse genome mapping. Mamm. Genome 8, 267-273 https://doi.org/10.1007/s003359900407
  6. Cho, G. J. 2006. Genetic relationship among the Korean native and alien horses estimated by microsatellite polymorphism. Asian-Aust. J. Anim. Sci. 19, 784-788 https://doi.org/10.5713/ajas.2006.784
  7. Cho, G. J. 2006. Detection of unusual allele in D system of red cell alloantigens found in the Korean native horse. J. Life Sci. 16, 1109-1111 https://doi.org/10.5352/JLS.2006.16.7.1109
  8. Cho, G. J. and B. H. Kim. 2000. Studies on blood types in Thoroughbred horses. Kor. J. Vet. Res. 40, 683-689
  9. Coogle, L., E. Bailey, R. Reid and M. Russ. 1996. Equine dinucleotide repeat polymorphisms at loci LEX002, -003, -004, -005, -007, -008, -009, -010, -011, -013 and -014. Anim. Genet. 27, 126-127
  10. Dimsoski, P. 2003. Development of a 17-plex microsatellite polymerase chain reaction kit for genotyping horses. Croat. Med. J. 44, 332-335
  11. Eggleston-Stott, M., A. L. DelValle, M. Bautista, D. Dileanis, E. Wictum and A. T. Bowling. 1997. Nine equine dinucleotide repeats at microsatellite loci UCDEQl36, UCDEQ405, UCDEQ412, UCDEQ425, UCDEQ437, UCDEQ467, UCDEQ 487, UCDEQ502 and UCDEQ505. Anim. Genet. 28, 370-371 https://doi.org/10.1111/j.1365-2052.1997.tb03270.x
  12. Ellegren, H., M. Johansson, K. Sandberg and L. Andersson. 1992. Cloning of highly polymorphic microsatellites in the horse. Anim. Genet. 23, 133-142 https://doi.org/10.1111/j.1365-2052.1992.tb00032.x
  13. Glowatzki-Mullis, M. L., J. Muntwyler, W. Pfister, E. Marti, S. Rieder, P. A. Poncet and C. Gaillard. 2005. Genetic diversity among horse populations with a special focus on the Franches-Montagnes breed. Anim. Genet. 37, 33-39 https://doi.org/10.1111/j.1365-2052.2005.01376.x
  14. Guerin, G., M. Bertaud and Y. Amigues. 1994. Characterization of seven new horse microsatellite: HMS1, HMS2, HMS3, HMS5, HMS6, HMS7 and HMS8. Anim. Genet. 25, 62
  15. Irvin, Z., J. Giffard, R. Brandon, M. Breen and K. Bell. 1998. Equine dinucleotide repeat polymorphisms at loci ASB21, 23, 25 and 37-43. Anim. Genet. 29, 67
  16. Kim, N. Y., S. S. Lee and Y. H. Yang. 2002. Polymorphisms of the exons 13, 15 and 16 of transferring gene in Cheju horses. J. Anim. Sci. & Technol. (Kor.) 44, 391-398 https://doi.org/10.5187/JAST.2002.44.4.391
  17. Marklund, S., H. Ellegren, S. Eriksson, K. Sandberg and L. Andersson, 1994. Parentage testing and linkage analysis in the horse using a set of highly polymorphic microsatellites. Anim. Genet. 25, 19-23 https://doi.org/10.1111/j.1365-2052.1994.tb00399.x
  18. Marshall, T. C., J. Slate, L. Kruuk and J. M. Pemberton. 1998. Statistical confidence for likelihood-based paternity inference in natural populations. Molecular Ecology 7, 639-655 https://doi.org/10.1046/j.1365-294x.1998.00374.x
  19. Menitti-Raymond, M. A., V. A. David, L. L. Wachter, J. M. Butler and S. J. O'Brien. 2005. An STR forensic typing system for genetic individualization of domestic cat (Felis catus) samples. J. Forensic Sci. 50, 1061-1070
  20. Saitou, N. and M. Nei. 1987. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol. Biol. Evol. 4, 406-425
  21. Singh, A., A. Gaur, K. Shailaja, B. S. Bala and L. Singh. 2004. A novel microsatellite (STR) marker for forensic identification of big cats in India. Forensic Sci. Int'l. 141, 143-147 https://doi.org/10.1016/j.forsciint.2004.01.015
  22. Tozaki, T., N. Takezaki, T. Hasegawa, N. Ishida, M. Kurosawa, M. Tomita, N. Saitou and H. Mukoyama. 2003. Microsatellite variation in Japanese and Asian horses and their phylogenetic relationship using a European horse outgroup. J. Heredity 94, 374-380 https://doi.org/10.1093/jhered/esg079
  23. Tozaki, T., H. Kakoi, S. Mashima, K. J. Hirota, T. Hasegawa, N. Ishida, N. Miura, N. H. Choi-Miura and M. Tomita. 2001. Population study and validation of paternity testing for Thoroughbred horses by 15 micro satellite loci. J. Vet. Med. Sci. 63, 1191-1197 https://doi.org/10.1292/jvms.63.1191
  24. van Haeringen, H., A. T. Bowling, M. L. Stott, J. A. Lenstra and K. A. Zwaagstra. 1994. A highly polymorphic horse microsatellite locus: VHL20. Anim. Genet. 25, 207
  25. Yoon, D. H., H. S. Kong, J. D. Oh, J. H. Lee, B. W. Cho, J. D. Kim, K. J. Jeon, C. Y. Jo, G. J. Jeon and H. K. Lee. 2005. Establishment of an individual identification system based on microsatellite polymorphisms in Korean cattle (Hanwoo). Asian-Aust. J. Anim. Sci. 18, 762-766 https://doi.org/10.5713/ajas.2005.762

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