Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Effects of glucose on metabolism and Insulin-like growth factor binding-3 expression in human fibroblasts.

사람의 섬유아세포에서 glucose 농도가 물질대사 및 Insulin-like growth factor binding protein-3의 발현에 미치는 영향

  • Ryu, Hye-Young (Faculty of Food Science and Biotechnology, Pukyung National University) ;
  • Hwang, Hye-Jung (Faculty of Food Science and Biotechnology, Pukyung National University) ;
  • Kim, In-Hye (Faculty of Food Science and Biotechnology, Pukyung National University) ;
  • Ryu, Hong-Soo (Faculty of Food Science and Biotechnology, Pukyung National University) ;
  • Nam, Taek-Jeong (Faculty of Food Science and Biotechnology, Pukyung National University)
  • 류혜영 (부경대학교 식품생명공학부) ;
  • 황혜정 (부경대학교 식품생명공학부) ;
  • 김인혜 (부경대학교 식품생명공학부) ;
  • 류홍수 (부경대학교 식품생명공학부) ;
  • 남택정 (부경대학교 식품생명공학부)
  • Published : 2007.05.25
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Abstract

Insulin-like growth factor-I(IGF-I) has significant insulin-like anabolic effects which include the stimulation of glucose and amino acid uptake, as well as protein and glycogen synthesis. IGFs exist in serum and other biological fluids as complexes bound to a family of structurally related insulin-like growth factor binding proteins(IGFBPs). Six human IGFBPs can modulate the effects of IGFs on target tissues by several mechanisms, including altering the serum's half-life and the transcapillary transport of IGFs, as well as changing the availability of IGFs to specific cell surface receptors. Human fibroblasts secrete IGFBPs that can modify IGF-I action. Previous to our study using either Northern blotting, and Western blotting have shown that fibroblasts express mRNA IGFBP-3, -4, and -5, and synthesize these proteins. In addition, fibroblast cell lysates revealed that the IGFBP-3 was most abundant. For these reasons, we undertook to gain further insight into the effects of high and low glucose incubation condition on metabolism and IGFBP-3 expression. In results of metabolites and IGFBP-3 expression in GM10 cells cultivated with various glucose concentration, the consumption of glucose and accumulation of triglyceride were increased in condition of high glucose, and total protein level was decreased. in the course of time. After 5 days incubation, levels of free amino acid in medium containing glucose of high concentration glucose were higher than in conditions of low glucose. Although the levels of IGFBP-3 protein and mRNA levels were increased in low glucose, and IGFBP-3 was not affected by any pretense. Taken together, we suggest that the study of growth factors, like IGFs, might be a possible model of diabetes militus in cell, although the results in cell models were not in accord with in vivo.

사람의 섬유아세포인 GM10을 사용하여 glucose의 배양조건에 다른 물질대사 및 IGFBP-3 발현을 살펴본 결과, glucose 농도에 따른 glucose 소비와 triglyceride 축적 수준은 고농도 glucose 배양 조건에서 증가한 반면, 총 단백질 함량은 고농도 glucose배양 조건에서 시간의 경과에 따라 감소하였다. 또한 고농도 glucose 배양 조건에서 5일 동안 배양한 세포 배양액내의 유리아미노산 함량은 저농도보다 고농도 glucose 배양 조건에서 높게 나타났다. IGFBP-3 단백질 수준과 mRNA수준은 저농도 glucose배양 조건에서 증가하였으나, IGFBP-3 단백질 분해효소에 따른 영향은 없었다. 이상의 결과에서 glucose 배양조건에 따른 물질대사는 부분적으로 세포를 이용한 당뇨병 모델 실험에서 in vivo와 같은 결과를 얻지 못하였지만, IGFs와 같은 세포 성장인자에 대한 연구를 통해 세포수준의 당뇨병 모델화가 가능하다고 여겨진다.

Keywords

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