Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Influence of Nitrate Against Effect of Cadmium on Growth and Rubisco in Seedling of Tobacco.

담배 유식물의 생장과 Rubisco에 미치는 카드뮴의 효과에 대한 질산염의 영향

  • Published : 2007.05.25
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Abstract

This investigation was performed to study the influence of Cd and nitrate on growth, and chlorophyll and photosynthetic enzymes in seedling of tobacco. Growth inhibition by Cd was not recovered by nitrate. Chlorophyll levels were reduced by Cd. The combination of Cd and low concentration of nitrate decreased the chlorophyll content compared to that in plants exposed only to Cd. Activity and content of rubisco at Cd treatment was significantly lesser than in plants receiving no treatment, These data suggest that rubisco activity was associated with an amount of rubisco protein, and that the activation and synthesis of rubisco is inhibited by Cd. Both the activity and content of rubisco decreased by Cd were more decreased by nitrate. A similar change pattern was also observed in activity and content of rubisco activase. These results suggest that Cd- and nitrate-induced changes of rubisco could be correlated with rubisco activase, and that nitrate was concerned in not only the activation and synthesis of rubisco directly, but also rubisco activase leading to a large change in rubisco.

담배 유식물의 생장, 엽록소 및 광합성 효소에 대한 카드뮴과 질산염의 영향에 대해 연구하였다. 카드뮴에 의한 억제된 생장은 질산염에 의해 회복되지 않았다. 엽록소 a와 b의 함량은 카드뮴에 의해 감소하였으며, 카드뮴과 질산염 혼합처리구는 카드뮴 처리구보다 엽록소 함량이 감소하였다. 카드윰 처리구에서의 rubisco의 활성과 함량은 대조구에서의 함량보다 현저히 작았다. 이는 rubisco의 활성이 함량과 연관되어 있으며, rubisco의 활성화와 합성이 카드뮴에 의해 저해된다는 것을 의미한다. 카드뮴에 의해 감소된 rubisco의 활성과 함량은 질산염에 의해 더욱 더 감소되었다. 이와 비슷한 변화가 rubisco activase의 활성과 함량에서도 관찰되었다. 이 결과들은 카드뮴과 질산염에 의해 유도되는 rubisco의 변화가 rubisco activase와 서로 연관되어 있음을 의미하며, 질산염은 직접적으로 rubisco의 활성화와 합성에 관여 할 뿐만 아니라 rubisco activase를 통해서도 rubisco의 수준에 관여하는 것으로 해석된다.

Keywords

References

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