생명과학회지 (Journal of Life Science)

  • 제17권5호
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  • Pages.641-645
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  • 2007
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  • 1225-9918(pISSN)
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  • 2287-3406(eISSN)
한국생명과학회 (Korean Society of Life Science)
권호 표지
DOI QR코드

DOI QR Code

Ethyl methane sulfonate(EMS)에 의해 변이된 애기장대 종자 집단으로부터 염 내성 돌연변이체 선발 및 특성 분석

Isolation and characterization of ethyl methane sulfonate(EMS) Arabidopsis mutants capable of germination under saline conditions.

  • 정문수 (전남대학교 식물생명공학부 및 농업식물 스트레스 센터) ;
  • 정정성 (전남대학교 식물생명공학부 및 농업식물 스트레스 센터) ;
  • 김철수 (전남대학교 식물생명공학부 및 농업식물 스트레스 센터)
  • Chung, Moon-Soo (Department of Plant Biotechnology and Agricultural Plant Stress Research Center, Chonnam National University) ;
  • Chung, Jung-Seong (Department of Plant Biotechnology and Agricultural Plant Stress Research Center, Chonnam National University) ;
  • Kim, Cheol-Soo (Department of Plant Biotechnology and Agricultural Plant Stress Research Center, Chonnam National University)
  • 발행 : 2007.05.25
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초록

염 내성 돌연변이체를 선발하기 위하여, ethyl methane sulfonate(EMS)로 처리된 돌연변이 종자 집단을 사용하였다. 150 mM NaCl 고염 스트레스 하에서 종자 발아 내성을 보이는 세 종류의 EMS 돌연변이체를 선발하였다. 세 종류의 EMS 돌연변이체들 중, salt tolerance 42-14(sto42-14) 돌연변이체는 175 mM NaCl 고농도에서 종자 발아율이 대조구(WT)에 비해 7배 이상의 높은 발아율을 보였다. 또한 내염성 sto42-14 돌연변이체는 glucose(Glc)에 대해서도 비감수성을 갖고 있음을 관찰되었고, 흥미롭게도 sto42-14돌연변이체에 $20{\mu}M$ gibberellin(GA)을 처리한 결과, 대조구에 비해 하배축과 뿌리의 생장이 억제됨을 관찰할 수 있었다. 이러한 결과를 바탕으로, 고염 내성 sto42-14 돌연변이체는 Glc 뿐만 아니라 GA호르몬 반응에도 관련되어져 있음을 알 수 있다.

We conducted a seed germination screening under saline conditions to identify salt tolerance(sto) mutants with ethyl methane sulfonate(EMS) mutagenesis seed pool. During the screening, we identified three mutant lines that seemed to confer elevated salt tolerance in high concentrations of NaCl. At 175 mM NaCl, germination rate of sto42-14 mutant(one of the EMS salt tolerance mutants) was 7-fold higher than that of wild-type plants. Interestingly, sto42-14 mutant exhibited insensitivity to high glucose concentration and growth inhibition to gibberellin. Our results suggest that sto42-14 is involved in salt stress tolerance as well as in glucose and gibberellin response in Arabidopsis.

키워드

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