Synergism in Antifungal Activity against Candida and Trichophyton Species in Combination with the Essential Oil of Coriandrum sativum L. and Antibiotics

  • Lim, Sook (College of Pharmacy, Duksung Women's University) ;
  • Shin, Seung-Won (College of Pharmacy, Duksung Women's University)
  • Published : 2007.03.31

Abstract

To determine whether the essential oil from Coriandum sativum and its main component, linalool, exhibit antifungal activity, we employed a broth dilution assay and disk diffusion test using common pathogenic Candida and Trichophyton species. Both coriander oil and linalool significantly inhibited growth of the tested fungi, with minimal inhibitory concentrations (MICs) in the range of $0.03{\sim}2mg/ml$. Furthermore, in a checkerboard titer test, both the oil fraction and linalool exhibited synergism when combined with ketoconazole, with resultant FICIs ranging from 0.06 to 0.53. Notably, hyphal formation in C. albicans cells was obviously inhibited by C. sativum essential oil in this experiment.

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References

  1. Bidlack, W.R., Omaye, S.T., Meskin, M.S., and Topham, D.W., Phytochemicals as Bioactive Agents. Technomic publishing company, Lancaster (2000)
  2. Cox, S.D., Mann, C.M., and Markham, J.L., The mode of antimicrobial action of the essential oil of Melaleuca alternifolia (tea tree oil). J. Appl. Microbiol. 88, 170-175 (2000) https://doi.org/10.1046/j.1365-2672.2000.00943.x
  3. Cutler, J.E., Putative virulence factors of Candida albicans. Annu. Rev. Microbiol. 45, 187-218 (1991) https://doi.org/10.1146/annurev.mi.45.100191.001155
  4. Davidson, P.M. and Parish. M.E., Methods for testing the efficacy of food antimicrobials. Food Technol. 43, 148-155 (1989)
  5. Garg, S.C. and Dengre, S.I., Antifungal efficacy of some essential oils. Pharmazie 43, 141-142 (1998)
  6. Giordani, R., Trebaux, J., Masi, M., and Regli, P., Enhanced antifungal activity of ketoconazole by Euphorbia characias latex against Candida albicans. J. Ethnopharmacol. 78, 1-5 (2001) https://doi.org/10.1016/S0378-8741(01)00295-1
  7. Hammer, K., Carson, C.F., and Riley, T.V., In vitro activities of ketoconazole, econazole, miconazole and Melaleuca alternifolia (tea tree) oil against Malassezia species. Antimicrob. Agents. Chemother. 44, 467-469 (2000) https://doi.org/10.1128/AAC.44.2.467-469.2000
  8. Said, H.M., Saeed, A,, D'Silvia, L.A., Zubairy, H.N., and Bano, Z., Medicinal Herbal: A Textbook for Medical Students and Doctors. Volume 1. Hamdard Foundation Pakistan (1996)
  9. Shin, S. and Kang, C.A., Antifungal activity of the essential oil of Agastache rugosa Kuntze and its synergism with ketoconazole. Lett. Appl. Microbiol. 36, 1874-1878 (2003)
  10. White, R.L., Burgess, D.S., Manduru, M., and Bosso, J.A., Comparison of three different In vitro methods of detecting synergy: time-kill, checkerboard and E test. Antimicrob. Agents Chemother. 40, 1914-1918 (1996)
  11. Zhang, J.D., Xu, Z., Cao, Y.B., Chen, H.S., Yan, L., An, M.M., Gao, P.H., Wang, Y., Jia, X.M., and Jiang, Y.Y., Antifungal activities and action mechanisms of compounds from Tribulus terrestris L. J. Ethnopharmacol. 103, 76-84 (2006) https://doi.org/10.1016/j.jep.2005.07.006