Natural Product Sciences

The Korean Society of Pharmacognosy (한국생약학회)
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Hepatoprotective Activity of Ethanolic Extract of Bacopa monnieri Linn. Aerial Parts Against $CCl_4-induced$ Hepatotoxicity in Rats

  • Published : 2007.03.31
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Abstract

The ethanolic extract of Bacopa monnieri Linn. aerial parts were studied far its hepatoprotective effect on $CCl_4-induced$ hepatotoxic rats. The extract was found to decrease significantly $CCl_4-induced$ elevation of SGOT, SGPT, ALP, bilirubin and total cholesterol. But, it increased HDL-cholesterol level and liver weight with respect to $CCl_4$ toxic rats. The extract was also found to decrease significantly the $CCl_4-induced$ elevation of lipid peroxidation and increase the activity of antioxidant enzymes (SOD and CAT) and GSH level in the liver of extract treated rats when compared with $CCl_4$ induced rats. Histopatholosical profiles showed that the extract had significant protective effect against $CCl_4-induced$ liver injury, which corroborates the above findings. Hence it may be possible that the mechanism of hepatoprotection of the extract is due to its antioxidant effect.

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References

  1. Anonymous. Indian Herbal Pharmacopoeia, Vol. 1, Indian Drug Manufacturer's Association, Mumbai, 30-36 (1998)
  2. Arteel, G.E., Oxidants and antioxidants in alcohol-induced liver disease. Gastroenterology. 124, 778-790 (2003) https://doi.org/10.1053/gast.2003.50087
  3. Babu, B.H., Shylesh, B.S., and Padikkala, J., Antioxidant and hepatoprotective effect of Acanthus ilicifolius. Fitoterapia. 72, 272-277 (2001) https://doi.org/10.1016/S0367-326X(00)00300-2
  4. Basu, N., Rastogi, R.P., and Dhar, M.L., Chemical examination of Bacopa monnieri Wettst: Part III-Bacoside B. Indian J. Chem. 5, 84-86 (1967)
  5. Bautista, A.P., Free radicals, chemokines and cell injury in HIV-I and SIV infections and alcoholic hepatitis. Free Radic. Biol. Med. 31, 1527-1532 (2001) https://doi.org/10.1016/S0891-5849(01)00745-6
  6. Chatterjee, N., Rastogi, R.P., and Dhar, M.L., Chemical examination of Bacopa monnieri Part II. The constitution of Bacoside A. Indian J. Chem. 3, 24-29 (1965)
  7. Chopra, R.N., Nayar, S.L., and Chopra, I.C., Glossary of Indian Medicinal plants, National Institute of Science and Communication (CSIR), New Delhi, 32-33 (1986)
  8. Chottopadhyay, R.R. and Bondyopadhyay, M., Possible mechanism of hepatoprotective activity of Azadirachta indica leaf extract against paracetamol-induced hepatic damage in rats: Part III. Indian J. Pharmacol. 37, 184-185 (2005) https://doi.org/10.4103/0253-7613.16218
  9. Ellman, G.L., Tissue sulfhydryl groups. Arch. Biochem. Biophys. 82, 70-77 (1959) https://doi.org/10.1016/0003-9861(59)90090-6
  10. Fraga, C.G., Leibovitz, B.E., and Toppel, A.L., Lipid peroxidation measured as TBARS in tissue slices: Characterisation and comparision with homogenates and microsomes. Free Radic. Bio. Med. 4, 155-161 (1988) https://doi.org/10.1016/0891-5849(88)90023-8
  11. Ghosh, T., Maity, T.K., Bose, A., and Dash, G.K., Anthelmintic activity of Bacopa monnieri. Indian J. Nat. Prod. 21, 16-19 (2005)
  12. Halliwell, B., Gutteridge, J.M., and Cross, C.E., Free radicals, antioxidants, and human disease: where are we now. J. Lab. Clin. Med. 119, 598-620 (1992)
  13. Harborne, J.B., Phytochemical Methods-A Guide to Modern Techniques of Plant Analysis, Chapman and Hall, New York, 4-7 (1984)
  14. Hu, J., Lee, S.O., Hendrich, S., and Murphy, Quantification of the group B soyasaponins by high-performance liquid chromatography. J. Agri. Food Chem. 50, 2587-2594 (2002) https://doi.org/10.1021/jf0114740
  15. Kakkar, P., Das, B., and Viswanathan, P.N., A modified spectroscopic assay of superoxide dismutase. Indian J. Biochem. Biophys. 21, 130-132 (1984)
  16. Kamiyama, T., Sato, C., and Liu, J., Role of lipidperoxidation in acetaminophen induced hepatotoxicity; comparison with carbontetrachloride. Toxicol. Lett. 66, 7-12 (1993) https://doi.org/10.1016/0378-4274(93)90073-7
  17. King, J., The hydrolases-acid and alkaline phospatase. In, Practical Clinical Enzymology, ed. by D. Van, Nostrand company Ltd., London, 191-208 (1965)
  18. Kurata, M., Suzuki, M., and Agar, N.S., Antioxidant systems and erythrocyte life span in mammals. Biochem. Physiol. 106, 477-487 (1993)
  19. Leeuwenburgh, C. and Ji, L.L., Glutathione depletion in rested and exercised mice: biochemical consequence and adaptation. Arch. Biochem. Biophys. 316, 941-949 (1995) https://doi.org/10.1006/abbi.1995.1125
  20. Lowry, O.H., Rosebrough, N.J., Farr, A.L., and Randall, R.J., Protein measurement with the folin-phenol reagent. J. Biol. Chem. 193, 265-275 (1951)
  21. Maehly, A.C. and Chance, B., Methods of Biochemical Analysis, Vol. 1, Interscience, New York, 357-358 (1954)
  22. Malloy, H.T. and Evelyn, K.A., The determination of bilirubin with the photometric colorimeter. J. Biol. Chem. 119, 481-490 (1937)
  23. Manoj, B. and Aqueed, K., Protective effect of Lawsonia alba Lam., against $CCl_{4}$-induced hepatic damage in albino rats. Ind. J. Expt. Biol. 41, 85-87 (2003)
  24. Mondal, S.K., Chakraborty, G., Gupta, M., and Mazumder, U.K. Hepatoprotective activity of Diospyros malabarica bark in carbon tetrachloride intoxicated rats. Eur. Bull. Drug. Res. 13, 25-30 (2005)
  25. Osawa, T., Kavakishi, S., and Namiki, M., Antimutagenesis and anticarcinogenesis mechanisms II, Plenum, New York, 139-153 (1990)
  26. Rao, K.S. and Mishra, S.H., Anti-inflammatory and hepatoprotective activities of Sida rhombifolia Linn. Indian J Pharmacol. 29, 110-116 (1997)
  27. Raucy, J.L., Kraner, J.C., and Lasker, J., Bioactivation of halogenated hydrocarbons by cytochrome $P_{450}$ El. Crit. Rev. Toxicol. 23, 1-20 (1993) https://doi.org/10.3109/10408449309104072
  28. Rietman, S. and Frankel, S.A., colorimetric determination of serum glutamic oxaloacetic and glutamic pyruvic transaminases. Am. J. Clin. Pathol. 28, 56-63 (1957) https://doi.org/10.1093/ajcp/28.1.56
  29. Saraswat, B., Visen, P.K., Patnaik, G.K., and Dhawan, B.N., Anticholeststic effect of Picroliv, active hepatoprotective principle of Picrorhiza kurrooa against $CCl_{4}$ induced cholestasis. Indian J. Expt. Biol. 31, 316-318 (1993)
  30. Seth, U.K., Dadkar N.K., and Kamat, U.G., Selected Topics in Experimental Pharmacology, 1st edn. Kothari Book Depot, Bombay, 126-127 (1972)
  31. Trease, G.E. and Evans, W. C., Pharmacognosy, 12th edn. Bailliere Tindall, London, 735-738 (1983)
  32. Warnick, G.R., Nguyen, Y., and Albers, A.A., Comparison of improved precipitation methods for quantification of high density lipoprotein cholesterol. Clin. Chem. 31, 217-221 (1985)
  33. Yoshiki, Y. and Okubo, K., Active oxygen scavenging activity of DDMP (2,3-dihydro-2, 5-dihydroxy-6-methyl-4H-pyran-4-one) saponin in soybean seed. Biosci. Biotech. Biochem. 59, 1556-1557 (1995) https://doi.org/10.1271/bbb.59.1556
  34. Zimmerman, H.J. and Seeff, L.B., Enzymes in hepatic disease, Lea and Febiger, Philadelphia, 24-26 (1970)