생명과학회지 (Journal of Life Science)

한국생명과학회 (Korean Society of Life Science)
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디젤분진 및 미세분진이 천식마우스에서 기도 재구성에 미치는 효과

The Effects of Diesel Exhaust Particulates and Particulate Matters on the Airway Remodeling in the Asthma-induced Mice

  • 리천주 (건국대학교 수의과대학) ;
  • 이수진 (건국대학교 수의과대학) ;
  • 장양호 (건국대학교 수의과대학) ;
  • 박준홍 (건국대학교 수의과대학) ;
  • 박세종 (건국대학교 수의과대학) ;
  • 이정학 (건국대학교 수의과대학) ;
  • 최농훈 (건국대학교 수의과대학)
  • Li, Tianzhu (Konkuk University College of Veterinary Medicine) ;
  • Lee, Soo-Jin (Konkuk University College of Veterinary Medicine) ;
  • Jang, Yang-Ho (Konkuk University College of Veterinary Medicine) ;
  • Park, Jun-Hong (Konkuk University College of Veterinary Medicine) ;
  • Park, Se-Jong (Konkuk University College of Veterinary Medicine) ;
  • Lee, Jeong-Hak (Konkuk University College of Veterinary Medicine) ;
  • Choe, Nong-Hoon (Konkuk University College of Veterinary Medicine)
  • 발행 : 2007.02.28
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초록

천식이 유발된 Balb/c 마우스와 동일한 조건의 Il-10 KO 마우스에 천식의 원인으로 알려진 DEP와 지하철역내에서 채칩한 PM ($10mg/m^{3}$)을 inhalation chamber에 넣고 하루 4시간씩 5일간 흡입시킨 후 시료를 채취하여 기관지 상피하부의 섬유화정도와 술잔세포의 증식 정도를 살펴 천식증상의 악화에 DEP와 PM이 어떠한 영향을 미치는지 확인하였다. 본 실험의 결과 천식이 유발된 일반 Balb/c 마우스에 있어서는 DEP와 PM의 노출에 의하여 상피하부의 섬유화가 증가하였고, 술잔세포의 수도 증가함을 확인하였다. 그러나, Il-KO 마우스의 경우 상피하부의 섬유화정도와 술잔세포의 수가 거의 증가하지 않았다. 따라서, 본 결과는 IL-10에 대한 항체요법이 천식증상의 완화에 쓰일 수 있는 가능성을 암시하며, 한편 자동차 배기가스와 지하철 미세분진의 발생을 예방할 경우 천식과 관련한 세기관지의 염증을 완화시킬 수 있음을 간접적으로 증명한 것이라 할 수 있다.

This research investigated whether exposure of diesel exhaust particulate (DEP) and particulate matter (PM) effects on airway remodeling in asthma induced Balb/c and IL-10 knock out (KO) mouse. Mice were sensitized with intraperitoneal injection with ovalbumin, followed by challenges with intranasal ovalbumin. After that mice placed in inhalation chamber and exposed to DEP and $PM(10\;mg/m^3)$. The evidence of airway remodeling was assessed by masson's trichrome staining and PAS staining. The stainability of masson's trichrome and PAS reaction were increased in asthma-induced Baltic mice groups compared with control mice groups. More intensive stainability for masson's trichrome and PAS were appeared in the asthma-induced DEP and PM-exposed groups than asthama-induced groups. But, not significantly increased subepithelial fibrosis and the nember of goblet cell hyperplasia in asthma-induced IL-10 KO mice groups and asthma-induced+DEP and PM-exposed IL-10 KO mice than IL-10 KO mice groups. These results indirectly suggesting that exposure to DEP and PM in asthmatic patients might be aggravate clinical symptoms and IL-10 which seems to play a central role in allergic asthma. In conclusion, DEP and PM exposure might have additive effects on the ovalbumin- induced asthma in a murine model.

키워드

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