KSBB Journal

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Effects of Pluronic F-68 and Oxygen Vectors on the Cell Growth of Angelica gigas Nakai in Aqueous Two-Phase System

수성이상계에서 Pluronic F-68과 산소전달물질이 참당귀 현탁세포 증식에 미치는 영향

  • Cheon, Su-Hwan (Department of Biological Engineering, Inha University) ;
  • Lee, Kyoung-Hoon (Department of Biological Engineering, Inha University) ;
  • Kwon, Jun-Young (Department of Biological Engineering, Inha University) ;
  • Ryu, Hyun-Nam (Department of Biological Engineering, Inha University) ;
  • Kim, Dong-Il (Department of Biological Engineering, Inha University)
  • 전수환 (인하대학교 공과대학 생물공학과) ;
  • 이경훈 (인하대학교 공과대학 생물공학과) ;
  • 권준영 (인하대학교 공과대학 생물공학과) ;
  • 류현남 (인하대학교 공과대학 생물공학과) ;
  • 김동일 (인하대학교 공과대학 생물공학과)
  • Published : 2007.08.30
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Abstract

Pluronic F-68 and oxygen vectors were applied to increase the cell growth of Angelica gigas Nakai in aqueous two-phase system (ATPS). ATPS was composed of 3.6% (w/v) polyethylene glycol (PEG) 20,000 and 2.8% (w/v) crude dextran. n-Hexadecane, n-dodecane and FC-40 were used as oxygen vectors to enhance the oxygen transfer in ATPS. With 2$\sim$10 g/L of Pluronic F-68, addition of of n-hexadecane and FC-40 significantly enhanced the oxygen transfer rate as well as the maximum cell mass in a medium with ATPS. However, n-dodecane reduced the cell growth in all treatments. Maximum cell densities were increased up to 27.5% with 10 g/L of Pluronic F-68 and up to 40.2% with 8% (v/v) n-hexadecane compared to those of the controls without Pluronic F-68 and oxygen vectors. It was confirmed that the cell growth could be increased in ATPS using n-hexadecane.

본 연구에서는 수성이상계에서 Pluronic F-68과 산소전달물질을 적용하여, 물질전달과 산소전달을 증가시켜 당귀세포의 증식을 향상시켰다. 특히 산소전달물질인 n-hexadecane이 Pluronic F-68보다 수성이상계에서의 당귀 세포증식에 더 긍정적임을 확인하였다. 따라서 Pluronic F-68과 적절한 산소 전달물질의 첨가는 수성이상계 뿐만 아니라 대량배양을 위한 고농도배양 등에 효과적으로 적용가능하리라 사료된다.

Keywords

References

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