KSBB Journal

  • 제22권5호
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  • Pages.282-287
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  • 2007
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  • 1225-7117(pISSN)
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  • 2288-8268(eISSN)
한국생물공학회 (The Korean Society for Biotechnology and Bioengineering)
권호 표지

PKA Inhibitor KT5720, Suppressed Cytoskeletal Components Effect by Vesicular Stomatitis Virus, but did not Affect the Viral Replication

  • Kim, Young-Sook (Department of Oriental Medical Food and Nutrition, Asia University)
  • 발행 : 2007.10.30
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초록

The antiviral mechanism of KT5720 is known to inhibit the cAMP-dependent protein kinase (PKA), on the VSV infection in BHK-21 cell cultures. The virus inducted CPE (cell rounding) was almost completely suppressed by KT5720 at 5 uM. The inhibitor, however, did not affect the replication of the virus and the synthesis of viral macromolecules. Immunological studies showed the viral matrix (M) protein displayed intimate association with the cytoskeletal components and probably the cell rounding. KT5720, did not block the cytoskeletal disruption, while the cell rounding was suppressed. These observations suggest that the interaction between the viral M protein and the cytoskeletal components may not be enough to cause the morphological change of the cell. And, the KT5720-sensitive function may be involved in developing the VSV-induced CPE, but not essential for the virus replications.

키워드

참고문헌

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