Effects of Rubus coreanus Miquel on the Expressions of Tyrosinase, TRP-1 and TRP-2 in B16 Melanoma Cells

복분자가 B16 세포주의 Tyrosinase, TRP-1 and TRP-2 발현에 미치는 영향

  • Oh, Se-Mi (Department of Herbal Resources, Professional Graduate School of Oriental Medicine, Wonkwang University) ;
  • Mun, Yeun-Ja (Department of Herbal Resources, Professional Graduate School of Oriental Medicine, Wonkwang University) ;
  • Woo, Won-Hong (Department of Herbal Resources, Professional Graduate School of Oriental Medicine, Wonkwang University)
  • 오세미 (원광대학교 한의학전문대학원 한약자원개발학과) ;
  • 문연자 (원광대학교 한의학전문대학원 한약자원개발학과) ;
  • 우원홍 (원광대학교 한의학전문대학원 한약자원개발학과)
  • Published : 2007.12.25

Abstract

Melanogenesis is induced mainly by ultraviolet radiation of sunlight and ${\alpha}-melanocyte$-stimulating hormone (${\alpha}-MSH$) which binds to a specific G protein coupled receptor. The purpose of this study was to investigate the mechanism of melanogenesis inhibition in B16/F10 cells by methanol extract of Rubus coreanus Miquel (RCM). In the present study, ${\alpha}-MSH$ and forskolin led to a stimulation of melanin synthesis that appeared to result from an increased tyrosinase activity and melanin content. However, RCM inhibited the ${\alpha}-MSH$- and forskolin-induced melanin synthesis. In addition, RCM abolished the ${\alpha}-MSH$- and forskolin-induced cytoplasmic dendricity. Regarding protein levels of the melanogenic enzymes, the amounts of tyrosinase and tyrosinase-related protein 1 (TRP-1) were increased after incubation with α-MSH and forskolin. The treatment of RCM decreased the ${\alpha}-MSH$- and forskolin-induced expression levels of tyrosinase and TRP-1. Based on these findings, it is likely that RCM exerts its depigmenting effects in B16/F10 cells through the suppression of tyrosinase and TRP-1 expression, which are key enzymes for melanogenesis.

Keywords

References

  1. Korner, A., Pawelek, J. Mammalian tyrosinase catalases three reactions in the biosynthesis of melanin. Science 217: 1163-1165, 1982 https://doi.org/10.1126/science.6810464
  2. Fuller, B.B., Meyskens, F.L. Jr. Endocrine responsiveness in human melanocyte and melanoma cells in culture. J. Natl. Cancer Inst. 66: 799-802, 1981
  3. Naeyaert, J.M., Eller, M., Gordon, P.R., Park, H.Y., Gilchrest, B.A. Pigment content of cultured human melanocytes does not correlate with tyrosinase message level. British J. Derma. 125: 297-303, 1991 https://doi.org/10.1111/j.1365-2133.1991.tb14161.x
  4. Richard, A.S. Human pigmentation genes and their response to solar UV radiation. Fundamental and Molecular Mechanism of Mutagenesis. 422: 69-76, 1998 https://doi.org/10.1016/S0027-5107(98)00176-6
  5. Hunt, G., Donatien, P.D., Lunec, J. Cultured human melanocytes respond to MSH peptides and ACTH. Pigment Cell Res 7: 217-221, 1994 https://doi.org/10.1111/j.1600-0749.1994.tb00052.x
  6. 정태현. 한국식물도감. 교육사, 1955
  7. 이미경, 이현수, 최근표, 오덕환, 김종대, 유창연, 이현용. 복분자 열매 추출물의 유용 생리활성 탐색. Korean J. Medicinal Crop Sci. 11(1):5-12, 2003
  8. 정승원, 이남경, 김석중, 한대석. Tyrosinase 활성을 저해하는 식물체의 탐색. 한국식품과학회지 27(6):891-896, 1995
  9. 조수민, 김지헌, 이민원. 탄닌화합물의 tyrosinase 억제 활성. 생약학회지 32(1):68-71, 2001
  10. Predminda, S., Philippe, D.D., Sadia, Q., Takeshi, K., Hearing, V.J., Jean-jacquees, P., Seth, J.O. Identification and characterization of a melanocyte-specific novel 65kDa peripheral membrane protein. Eur. J. Biochem. 266: 924-934, 1999 https://doi.org/10.1046/j.1432-1327.1999.00930.x
  11. Hosoi, J., Abe, E., Suda, T., Kuroki, T. Regulation of melanin synthesis of B16 mouse melanoma cells by 1 $\alpha$, 25-dehycroxyvitamin D3 and retinoic acid. Cancer Res. 45: 1474-1478, 1985
  12. Martinez-Esparza, M., Jimenez-Cervantes, C., Solano, F., Lozano, J.A., Garcia-Borron, J.C. Mechanism of melanogenesis inhibition by tumor necrosis factor-alpha in B16F10 mouse melanoma cells. Eur. J. Biochem. 255: 139-146, 1998 https://doi.org/10.1046/j.1432-1327.1998.2550139.x
  13. Park, H.Y., Gilchrest, B.A. Signaling pathways mediating melanogenesis. Cell Mol. Biol. 45: 919-930, 1999
  14. Hearing, V.J. The melanosome: the perfect model for cellular responses to the cellular responses to the environment. Pigment Cell Res. 13: 23-34, 2000 https://doi.org/10.1034/j.1600-0749.13.s8.7.x
  15. Tachibana, M. MITF: A stream flowing for pigment cells. Pigment Cell Res. 13: 60-69, 2000 https://doi.org/10.1034/j.1600-0749.2000.130203.x
  16. Lerner, A.B. The discovery of the melanotropins:a history of pituitary endocrinology. Ann NY Acad. Sci. 680: 1-12, 1993 https://doi.org/10.1111/j.1749-6632.1993.tb19670.x
  17. Ranson, M., Psen, S., Mason, R.S. Human melanocytes as a target tissue for hormones : in vitro studies with 1 $\alpha$-25 dihydroxyvitamine D3, $\alpha$-melanocyte stimulating hormone, and beta estradiol. J Invest. Dermatol. 91: 593-598, 1988 https://doi.org/10.1111/1523-1747.ep12477126
  18. Im, S., Kim, J., On, W.Y., Kang, W.H. Increased expression of $\alpha$-Melanocyte-stimulating hormone in the lesionl skin of melasma. Br. J. Dermatol. 146: 165-167, 2002 https://doi.org/10.1046/j.1365-2133.2002.4513_3.x
  19. Busca, R., Ballotti, R. Cyclic AMP: a key messenger in the regulation of skin pigmentation. Pigment Cell Res. 13: 60-69, 2000 https://doi.org/10.1034/j.1600-0749.2000.130203.x
  20. Abdel-Malek, Z.A., Swope, V.B., Suzuki, I., Harriger, D., Boyce, S.T., Urabe, K., Hearing, V.J. Mitogenic and melanogenic stimulation of normal human melanocytes by melanotropic peptides. Proc. Natl. Acad. Sci. USA 92: 1789-1793, 1995
  21. Fuller, B.B., Meyskens, F.L. Jr. Endocrine responsiveness in human melanocyte and melanoma cells in culture. J. Natl. Cancer Inst. 66: 799-802, 1981
  22. Takechi, Y., Hera, I., Naftzger, C., Xu, Y., Houghton, A.N. A melanosomal membrane protein is a cell surface target for melanoma therapy. Clin. Cancer Res. 2: 1987- 1842, 1996
  23. Hearing, V.J., Tsukamoto, K., Urabe, K. et al. Functional properties of cloned melanogenic proteins. Pigment Cell Res. 5: 264-270, 1992. https://doi.org/10.1111/j.1600-0749.1992.tb00547.x
  24. Kobayashi, T., Imokawa, G., Bennett, D.C., Hearing, V.J. Tyrosinase stabilization by Tyrp1(the brown locus protein). J Biol. Chem. 273: 31805-31808, 1998