Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
권호 표지

Water Extracted Evodiae Fructus Possesses Immunomodulatory Activities on Cyclophosphamide Induced Immunesuppression

오수유 열수추출액이 Cyclophosphamide 유도 면역억제에 미치는 효과

  • Lee, Young-Sun (Department of Traditional Health Resource Development, College of Sung Duk) ;
  • Lee, Geum-Hong (Department of Pathology, College of Oriental Medicine, Deagu Haany University) ;
  • Park, Jong-Hyun (Department of Pathology, College of Oriental Medicine, Deagu Haany University) ;
  • Kwon, Young-Kyu (Department of Physiology, College of Oriental Medicine, Deagu Haany University) ;
  • Shin, Sang-Woo (School of Oriental Medicine, Pusan National University)
  • 이영선 (성덕대학 전통건강자원개발과) ;
  • 이금홍 (대구한의대학교 한의과대학 병리학교실) ;
  • 박종현 (대구한의대학교 한의과대학 병리학교실) ;
  • 권영규 (대구한의대학교 한의과대학 생리학교실) ;
  • 신상우 (부산대학교 한의학전문대학원)
  • Published : 2007.12.25
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Abstract

Evodiae Fructus(EF) has been used as Traditional medicine for the treatment of headache, abdominal pain, hemorrhage, and menorrhae in many Asian countries. The present study was conducted to investigate the immunomodulatory effect on cyclophosphamide(CY)-induced immunesuppression of water extracted EF(EFE). In the mouse spleen cell proliferation assay, EFE enhanced mitogenic activity and restored the CY-induced cell suppression. In the nitric oxide(NO) assay, EFE inhibited NO production and iNOS protein levels in LPS-stimulated RAW 264.7 cells. In the GC-MS analysis, many ingredients of EFE were detected by solvents. These results indicated that EFE can modulate immune response through immune cell proliferation, the regulation of NO production and the inhibition of CY-induced immunotoxicity.

Keywords

References

  1. 전국한의과대학 본초학교수. 본초학. 서울, 영림사, pp 337-338, 1991
  2. 이성우, 황방연, 김세은, 김환묵, 김영호, 이경순, 이정준, 노재섭. 약용식물 오수유로부터 다제약제 내성 조절물질의 분리. 생약학회지 26(4):344-348, 1995
  3. 정수연, 박윤주, 정면우, 임화경, 강주희, 최기환, 김주일. 오수유가 선천성고혈압휜쥐의 혈압에 미치는 영향. 응용약물학회지 8(4):305-310, 2000
  4. 김성은, 김대근, 신태용, 임종필, 엄동욱. 흡광도 측정법에 의한 오수유탕 중 오수유 알칼로이드의 정량. 생약학회지 35(1):88-91, 2004
  5. 김상태, 안승희, 김정도, 김영균. 오수유 MeOH 추출물이 Cyanide에 의한 신경세포의 보호효과에 미치는 영향. 생약학회지 34(4):282-287, 2003
  6. Pellati, F., Benvenuti, S., Yoshizaki, F., Bertelli, D., Rossi, M.C. Headspace solid-phase microextraction-gas chromatography--mass spectrometry analysis of the volatile compounds of Evodia species fruits. J Chromatogr A 1087(1-2):265-273, 2005 https://doi.org/10.1016/j.chroma.2005.01.060
  7. Tsai, T.H., Chang, C.H., Lin, L.C. Effects of Evodia rutaecarpa and rutaecarpine on the pharmacokinetics of caffeine in rats. Planta Med 71(7):640-645, 2005 https://doi.org/10.1055/s-2005-871270
  8. Liao, C.H., Pan, S.L., Guh, J.H., Chang, Y.L., Pai, H.C., Lin, C.H., Teng, C.M. Antitumor mechanism of evodiamine, a constituent from Chinese herb Evodiae fructus, in human multiple-drug resistant breast cancer NCI/ADR-RES cells in vitro and in vivo. Carcinogenesis 26(5):968-975, 2005 https://doi.org/10.1093/carcin/bgi041
  9. Ueng, Y.F., Tsai, T.H., Don, M.J., Chen, R.M., Chen, T.L. Alteration of the pharmacokinetics of theophylline by rutaecarpine, an alkaloid of the medicinal herb Evodia rutaecarpa, in rats. J Pharm Pharmacol 57(2):227-232, 2005 https://doi.org/10.1211/0022357055489
  10. Wang, C., Wang, M.W., Tashiro, S., Onodera, S., Ikejima, T. Roles of SIRT1 and phosphoinositide 3-OH kinase/protein kinase C pathways in evodiamine-induced human melanoma A375-S2 cell death. J Pharmacol Sci 97(4):494-500, 2005 https://doi.org/10.1254/jphs.FPJ04055X
  11. Ueng, Y.F., Yu, H.J., Lee, C.H., Peng, C., Jan, W.C., Ho, L.K., Chen, C.F., Don, M.J. Identification of the microsomal oxidation metabolites of rutaecarpine, a main active alkaloid of the medicinal herb Evodia rutaecarpa. J Chromatogr A 1076(1-2):103-109, 2005 https://doi.org/10.1016/j.chroma.2005.04.021
  12. Lee, S.K., Kim, N.H., Lee, J., Kim, D.H., Lee, E.S., Choi, H.G., Chang, H.W., Jahng, Y., Jeong, T.C. Induction of cytochrome P450s by rutaecarpine and metabolism of rutaecarpine by cytochrome P450s. Planta Med 70(8):753-757, 2004 https://doi.org/10.1055/s-2004-827207
  13. Fei, X.F., Wang, B.X., Li, T.J., Tashiro, S., Minami, M., Xing de, J., Ikejima, T. Evodiamine, a constituent of Evodiae Fructus, induces anti-proliferating effects in tumor cells. Cancer Sci 94(1):92-98, 2003 https://doi.org/10.1111/j.1349-7006.2003.tb01358.x
  14. Kobayashi, Y. The nociceptive and anti-nociceptive effects of evodiamine from fruits of Evodia rutaecarpa in mice. Planta Med 69(5):425-428, 2003 https://doi.org/10.1055/s-2003-39701
  15. Zhang, Y., Wu, L.J., Tashiro, S., Onodera, S., Ikejima, T. Intracellular regulation of evodiamine-induced A375-S2 cell death. Biol Pharm Bull 26(11):1543-1547, 2003 https://doi.org/10.1248/bpb.26.1543
  16. Senthikumar, S., Devaki, T., Manohar, B.M., Babu, M.S. Effect of squalene on cyclophophamide-induced toxicity. Clin Chim Acta 364: 335-342, 2005 https://doi.org/10.1016/j.cca.2005.07.032
  17. Artym, J., Zimecki, M., Kruzel, M. Normalization of peripheral blood cell composition by lactoferrin in cyclophosphamide-treated mice. Med Sci Monit 10(3):BR84-89, 2004
  18. 표명윤, 현수미, 양기숙. 상황버섯 추출물이 정상 마우스와 cyclophosphamide로 처리된 마우스의 체액성 면역기능에 미치는 양향. 응용약물학회지 9: 194-200, 2001
  19. 마진열, 하창수, 성현제, 지옥표. Cyclophosphamide로 Rat에 유도된 악성빈혈에 대한 숙지황의 증수에 따른 치료효능에 관한 연구. 생약학회지 32(3):325-334, 2005
  20. Zhang, Y., Wu, L.J., Tashiro, S., Onodera, S., Ikejima, T. Evodiamine induces tumor cell death through different pathways: apoptosis and necrosis. Acta Pharmacol Sin 25(1):83-89, 2004
  21. Huang, D.M., Guh, J.H., Huang, Y.T., Chueh, S.C., Chiang, P.C., Teng, C.M. Induction of mitotic arrest and apoptosis in human prostate cancer pc-3 cells by evodiamine. J Urol 173(1):256-261, 2005 https://doi.org/10.1097/01.ju.0000141587.72429.e3
  22. 정보섭, 신민교. 향약대사전. 서울, 영림사, p 786, 1990
  23. 중앙대사전. 서울, 성보사, p 1118, 1992
  24. 중초약학. 홍콩, 상무인서관, p 299, 1983
  25. 왕본상. 현대중약약리학. 천진, 천진과학기술출판사, p 608, 1997
  26. Lee, Y.S., Han, O.K., Park, C.W., Suh, S.I., Shin, S.W., Yang, C.H., Jeon, T.W., Lee, E.S., Kim, K.J., Kim, S.H., Yoo, W.K., Kim, H.J. Immunomodulatory effects of aqueous-extracted Astragali radix in methotrexate-treated mouse spleen cells. J. Ethno 84: 193-198, 2003 https://doi.org/10.1016/S0378-8741(02)00298-2
  27. 이영선, 권영규, 이금홍, 신상우. 건강 열수출액이 Methotrexate에 의해 유도된 마우스 면역억제 조절에 미치는 영향. 동의생리병리학회지 21(2):485-490, 2006
  28. Artym, J., Zimecki, M., Paprocka, M., Kruzel, M.L. Orally administered lactoferrin restores humoral immune response in immunocompromised mice. Immunology Letters 89: 9-15, 2003 https://doi.org/10.1016/S0165-2478(03)00102-0
  29. MacMicking, J., Xie, Q.W., nathan, C. Nitric oxide and macrophage function. Annu Rev immunol 15: 323-350, 1997 https://doi.org/10.1146/annurev.immunol.15.1.323
  30. Alderton, W.K., Cooper, C.E., Knowles, R.G. Nitric oxide synthases: structure, function and inhibition. Biochem J 357(pt 3):593-615, 2001 https://doi.org/10.1042/0264-6021:3570593